The disclosed disclosure is related to methods, compositions, and kits for targeting Adenovirus, Metapneumovirus, and/or Rhinovirus nucleic acid. Compositions include amplification oligomers and/or detection probe oligomers. Kits and methods comprise at least one of these oligomers. Methods include uniplex and multiplex amplification and detection reactions.

A primer for detecting fecal pollution in water, kit and high-throughput tracing method is disclosed, and belongs to the field of water pollution detection. The method mainly comprises the following steps: extracting DNA from water samples to be tested; using two pairs of universal primers for amplifying mitochondrial DNA to perform nested PCR amplification on the water sample DNA; performing high-throughput sequencing of the amplified products; performing annotation and alignment between sequencing data and a mitochondrial DNA database, and determining the sources of fecal pollution in water samples based on the alignment results. According to the present invention, nested PCR technology is utilized to amplify mitochondrial DNA with high sensitivity. The universal primers are combined with high-throughput sequencing, which can not only trace multiple sources of potential fecal pollution simultaneously, but also correspondingly quantify the degree of fecal pollution, thereby determining the main pollution source.

Provided herein are methods for generating single-cell molecular analysis comprising a) delivering one or more proximity dependent probes to a cell population, wherein each proximity dependent probe comprises a target binding region configured to bind a target RNA and a primer binding site region; b) linking bound proximity dependent probes; c) isolating single cells from the cell population in separate individual discrete volumes, the individual discrete volumes further comprising a primer pair and amplification reagents, wherein the primer pair binds to the primer binding sites of the ligation dependent probes, and wherein at least one primer comprises a barcode sequence that uniquely identifies the individual discrete volume; d) amplifying the ligated probes using the primer pair, wherein the barcode is incorporated into each resulting amplicon; and e) quantifying target RNAs in each individual cell based at least in part on sequencing the resulting amplicons.

Provided herein are methods of amplifying and detecting pathogens and drug resistance markers (e.g., antibiotic resistance genes) in complex samples (e.g., blood), as well as related panels and compositions (e.g., primers, probes, magnetic particles, systems, cartridges, and kits). The methods, panels, and compositions can be used for comprehensive detection of pathogens and drug resistance markers for patient identification, patient selection, optimization of therapies, and antimicrobial stewardship.

The present disclosure provides a method of treating a cancer in a patient who has undergone a first anti-cancer therapy. Also provided is a method for monitoring treatment response and minimum residual disease in a neoadjuvantly treated cancer patient. Methods for primer design are also disclosed. The present disclosure provides several tools for increasing the sensitivity and analytical precision of the disclosed methods for monitoring ctDNA.

The invention relates to a method for amplifying at least two distinct nucleic acid sequences present in a sample using a pair of primers (1) and a pair of primers (2). In particular, the first primer of the pair of primers (2) also comprises, in the (5′) position, the sequence of the first primer of the pair of primers (1), and the second primer of the pair of primers (2) also comprises, in the (5′) position, the sequence of the second primer of the pair of primers (1). The invention also relates to a kit and a detection kit capable of implementing said method and the uses thereof.

The present disclosure provides, among other things, primers and probes for detecting shedding of an AAV construct or fragment thereof in a subject. In some embodiments the primers are selected to generate an amplicon that comprises (i) a first strand comprising (1) a nucleotide sequence corresponding to the forward primer, and (2) a nucleotide sequence corresponding to a portion of the AAV construct or fragment thereof, (ii) a second strand comprising (1) a nucleotide sequence of the reverse primer, and (2) a nucleotide sequence that is complementary to the portion of the AAV construct or fragment thereof, or (iii) a combination thereof, where the portion of the AAV construct or fragment thereof comprises a nucleotide sequence that spans a junction between a regulatory element and the therapeutic gene of interest.

Embodiments of the present disclosure provide a reagent kit and a method for detecting renal cell carcinoma. The reagent kit includes a set of primer-probe mixes for detecting miRNAs in exosomes. The set of primer-probe mixes includes a reverse transcription primer R-23b for a specific reverse transcription miR-23b-5p target, a universal forward primer Ge—F, a specific reverse primer 23b-R, and a specific probe 23b-P; a reverse transcription primer R-34c for a specific reverse transcription miR-34c-5p target, a universal forward primer Ge—F, a specific reverse primer 34c-R, and a specific probe 34c-P; a reverse transcription primer R-210 for a specific reverse transcription miR-210-3p target, a universal forward primers Ge—F f, a specific reverse primer 210-R, and a specific probe 210-P; and a reverse transcription primer R-508 for a specific reverse transcription miR-508-3p target, a universal forward primer Ge—F, a specific reverse primer 508-R, and a specific probe 508-P.

The present disclosure belongs to the technical field of pathogen detection, in particular to loop-mediated isothermal amplification (LAMP) primer sets for detecting porcine susceptibility-related pathogenic bacteria, and a kit, a LAMP chip and use based on the same. The LAMP primer sets for detecting porcine susceptibility-related pathogenic bacteria include an Actinobacillus pleuropneumoniae primer set, a Haemophilus parasuis primer set, a Salmonella choleraesuis primer set, a Bordetella bronchiseptica primer set, a Pasteurella multocida primer set, a Streptococcus suis primer set, and an Erysipelothrix rhusiopathiae primer set.

Kits and methods for diagnosing risk of developing lung cancers and uses thereof are described. In a first aspect, described herein are lung cancer risk test kits that include reagents for measurement of multiple low VAF (defined as VAF<1%) mutants in a set of lung cancer driver genes; and, instructions therefor.