Provided herein is a method for mapping rolling circle amplification (RCA) products that contain unique identifier sequences. The method generally involves (a) producing a complex comprising population of grid oligonucleotide molecules and a population of RCA products that each have a unique RCA product identifier sequence, wherein the grid oligonucleotides are hybridized directly or indirectly via a splint to complementary sites in the RCA products; (b) extending the grid oligonucleotide molecules that are hybridized to two RCA products to add the complements of the unique RCA product identifier sequences from the two RCA products to the grid oligonucleotide molecules; (c) sequencing the extended grid oligonucleotides; (d) analyzing the sequences to identify which pairs of unique RCA product identifier sequence complements have been added onto the grid oligonucleotides; and (e) making one or more physical maps of the immobilized RCA products using the pairs of sequences identified in (d).

This invention relates to the field of detection of intestinal parasites from patient, food or environmental samples, preferably from a stool sample. Particularly, the present invention provides a polymerase chain reaction (PCR) based assay method for detection of intestinal parasite infection, particularly the infection of parasite species selected from a group consisting of Hymenolepis nana, Hymenolepis diminuta, Fasciolopsis buski, Encephalitozoon spp. (such as E. intestinalis, E. cuniculi and E. hellem), Enterocytozoon bieneusi, Enterobius vermicularis, Diphyllobothrium latum, Diphyllobothrium nihonkaiense, Schistosoma mansoni, Blastocystis hominis, Ancylostoma duodenale and liver worms, such as Clonorchis sinensis, Opisthorchis spp., and Metorchis spp. The present invention further provides materials such as primers, primer pairs and probes for use in the method of the invention. Preferably, the method of the invention is a multiplex real-time PCR assay for rapid determination of clinically important intestinal parasites.

A method determines a number of copies of a DNA sequence that is present in a fluid. The method includes a division step, a setting up step, an identification step, and an evaluation step. In the division step, at least some of the fluid is divided into at least two compartments. In the setting up step, a reaction condition is set up for the fluid divided into the at least two compartments in order to allow a reaction in each of the at least two compartments and to obtain a reaction result in each case. In the identification step, a signal, for example an optical signal, is identified that represents the reaction results of the reactions that may have taken place in the compartments. In the evaluation step, the optical signal is evaluated in order to determine the number of copies.

Provided is a method and a kit for detection of human papillomaviruses. Specifically, provided is a method for detecting at least 65 human papillomavirus genotypes. Also provided is a kit for detection of human papillomaviruses, which comprises one or more reagents capable of detecting at least 65 human papillomavirus genotypes.

Provided herein are compositions, kits, and methods for nucleic acid barcoding. The barcode compositions provided herein can be used to linearly, combinatorially, or spatially barcode a plurality of targets in a sample. Also provided herein is a device for use in a barcoding method provided herein comprising a light source and a sample holder.

A LAMP primer set includes original LAMP primers of FIP, BIP, F3, and B3, and at least one autonomy primer. The original LAMP primers target regions F3, F2, F1C, B1C, B2, and B3 on nucleic acids, and the regions F3, F2, F1, B1C, B2C and B 3 C are located in order from 5′ end to 3′ end of a forward strand of the nucleic acids. The primer FIP includes oligonucleotides targeting F1C and F2, and the primer BIP includes oligonucleotides targeting B1C and B2. The at least one autonomy primer targets a region located beyond a region from F3 to B3.

The disclosure provides gene fusions, gene variants, and novel associations with disease states, as well as kits, probes, and methods of using the same.

An assay is provided for the rapid detection of nucleic acids via a modified LAMP reaction coupled with colorimetric reporter utilizing a gold nanoparticle—peptide nucleic acid (AuNP-PNA) probe system.

The invention relates to methods for purifying and isolating at least one RNA molecule which interacts with an RNA-binding protein (RBP). The invention also provides nucleic acid adaptors and primers for use in such methods.

Provided herein are compositions, methods, and kits for detecting human picobirnavims (PBV). In certain embodiments, provided herein are PBV specific nucleic acid probes and primers, and methods for detecting PBV nucleic acid.