Disclosed are an enhanced efficient nitrogen-fixing composite microbial system added with non-nitrogen-fixing bacteria and application thereof, belonging to the technical field of agricultural microorganisms. The present disclosure provides enhanced efficient nitrogen-fixing bacteria, including at least one selected from a group of Klebsiella MNAZ1050, Citrobacter MNAZ1397 and Pseudomonas MNAZ228; also, the disclosed enhanced efficient nitrogen-fixing composite microbial system includes nitrogen-fixing bacteria and non-nitrogen-fixing bacteria, where the nitrogen-fixing bacteria includes at least one of the above three nitrogen-fixing bacteria, and the non-nitrogen-fixing bacteria includes at least one of Acinetobacter ACZLY512 and Kluyvera AZ981.

Disclosed herein include methods, compositions, and kits suitable for use in imaging of in situ gene expression. There are provided, in some embodiments, viral vector compositions. Disclosed herein includes a single viral vector comprising one or more gas vesicle assembly (GVA) gene(s) encoding one or more GVA protein(s), and one or more gas vesicle structural (GVS) gene(s) encoding one or more GVS protein(s). The one or more GVA protein(s) and the one or more GVS protein(s) can be capable of forming gas vesicles (GVs) upon expression in a cell.

Provided are a Bifidobacterium animalis subsp. lactis i797, a method for the separation and purification thereof, and a use thereof. The strain was is deposited in the China General Microbiological Culture Collection Center on Aug. 20, 2019, wherein the deposit address is Building 3, No. 1 Beichen West Road, Chaoyang District, Beijing, and the deposit number is CGMCC NO. 18403. The Bifidobacterium animalis subsp. lactis i797 can adjust the balance of intestinal flora, improve stool characteristics, and has a better survival rate in simulated digestive juice; in addition, after being stored at 37° C., a relatively high temperature which is suitable for the growth of lactic acid bacteria, same can successfully control post-acidification.

The present disclosure provides farmers a new platform for supplying nitrogen to their crops, which is based upon sustainable, biologically fixed nitrogen. The taught platform enables improved yield consistency across all cultivated acreage, irrespective of: weather, environment, or soil conditions. As a result of the increased yield consistency enabled by the taught disclosure, farmers have an increased degree of predictability for yield across each acre they plant, which was not possible with the synthetic nitrogen delivery paradigm of years past.

A fusion protein, an amino acid sequence thereof, a coding nucleotide sequence thereof, a preparation method thereof and a use thereof are in the technical field of agricultural biotechnology. The fusion protein contains or consists of at least three, four, five, six, seven, or eight same and/or different PAMP (Pathogen-Associated Molecular Pattern) polypeptides. Optionally, there is at least one linker or no linker between two adjacent PAMP polypeptides. A plurality of PAMP polypeptides are assembled into the fusion protein having multiple immune epitopes. The fusion protein may induce defense immune responses of plants, weaken infestation ability of pathogenic microorganisms and substantially improve the disease resistance of plants. The method for preparing the fusion protein combines technologies of PTI (PAMP-Triggered Immunity) mechanism and gene engineering to obtain the fusion protein having multiple immune epitopes can be used in preparation of plant immune PAMP polypeptides.

Disclosed herein are novel methods for hydrolyzing eggshell membrane (ESM). In one embodiment, the method includes cultivating thermophilic bacteria in a solution containing 1-10% (wt %) ESM to decompose the ESM into the ESM hydrolysate; wherein, the thermophilic bacteria grow on the ESM as their sole source of nutrient. In another embodiment, the method includes treating ESM with a keratinase in the presence of a reducing agent at a condition sufficient to produce the ESM hydrolysate, in which the keratinase, the reducing agent, and the ESM are present in a weight ratio of 1:120:600. The thus produced ESM hydrolysate is enriched in essential amino acids, collagen, peptides and glycosaminoglycans.

An iron-reducing Tessaracoccus oleiagri strain DH10 and applications of the iron-reducing Tessaracoccus oleiagri strain DH10 are provided. The Tessaracoccus oleiagri strain DH10 had been preserved in China center for type culture collection on Apr. 19, 2021, with a preservation number of CCTCC No: M 2021404. The iron-reducing Tessaracoccus oleiagri strain DH10 can efficiently reduce Fe(III) is separated and screened from oil reservoir environment. SEM analysis shows that it can effectively decompose lean iron montmorillonite minerals and inhibit expansion of clay. Moreover, core experiments show that biological agent of the Tessaracoccus oleiagri strain DH10 can reduce water sensitivity of reservoir core and water injection pressure, and can be applied to crude oil recovery to effectively improve crude oil recovery factor.

Microbial cell lines suitable for industrial-scale production of organic acids and methods of making and isolating such cell lines.

A nutrient-germinant composition to aid in spore germination and a method for increased spore germination efficiency. The composition comprises L-amino acids, D-glucose and/or D-fructose, a phosphate buffer, an industrial preservative, and may include bacteria spores or they may be separately combined for germination. The method comprises providing a nutrient-germinant composition and bacteria spores, preferably of one or more Bacillus species, and heating to a preferred elevated temperature range of 41° C. to 44° C. for an incubation period of around 2 to 60 minutes. The nutrient-germinant composition is preferably in a concentrated liquid form that is diluted just prior to initiating the germination/incubation method at the point of use. The method may also include dispensing a germinated spore solution to a point-of-use/consumption, such as animal feed, water, or bedding, or a wastewater system or drain.

Provided in this disclosure, in some embodiments, are methods and compositions for treating maple syrup urine disease (MSUD) and other conditions characterized by excessive branched-chain amino acids.