A continuous process for the synthesis of biodiesel from microalgae oil by transesterification with supercritical methanol, where the synthesis of biodiesel is carried out in a single transesterification step in a reactor that operates continuously, said process being ideal to be carried out at industrial scale since it can provide a continuous flow of biodiesel.

Disclosed is a mutant strain having the ability to produce polyhydroxybutyrate. The novel strain has a significantly high growth rate and an improved ability to produce PHB compared to existing PHB-producing cyanobacterial strains. Therefore, the novel strain is suitable for use in the production of PHB and the development of various products using PHB. In addition, the novel strain is useful as a photosynthetic strain for developing a PHB production process using industrial flue gas due to its ability to produce PHB from only CO.sub.2 without any additional organic carbon source. Also disclosed is a method for producing polyhydroxybutyrate using the mutant strain.

A method executed by a bioreactor system to cultivate a microalgae comprising a photoreceptor sensitive to a region of a visible spectrum under an autotrophic condition and a fermentation condition is described. A first process incubates the microalgae under the autotrophic condition and the fermentation condition simultaneously to allow the microalgae to grow and then removes the fermentation condition halfway through the process. A second process incubates the microalgae under the autotrophic condition and the fermentation condition simultaneously to allow the microalgae to grow. Both processes generate compounds, or functional proteins.

A method of manufacturing a transparent microbial energy device includes disposing a first transparent electrode, disposing a first hydrogel layer including an algal cell on the first transparent electrode, disposing a Nafion layer on the first hydrogel layer, disposing a second hydrogel layer including potassium ferricyanide on the Nafion layer, and disposing a second transparent electrode on the second hydrogel layer.

Presented herein are Synechococcus strains engineered to express the bacterial ethylene-forming enzyme (EFE) that exhibit unstable ethylene production due to toxicity and genomic instability induced by accumulation of the EFE-byproduct guanidine. Co-expression of EFE and Sll1077 significantly enhanced genomic stability and enabled the resulting Synechococcus strain GD-EFE7942 to achieve sustained high-level ethylene production. The engineered strains and methods disclosed herein are useful for guanidine degradation pathways and for ethylene bioproduction in cyanobacteria.

Provided herein, in some embodiments, are engineered cells and use of the same for increased hydrogen production. In particular, provided herein are genetically engineered cells comprising a polynucleotide encoding a fusion protein comprising a photosystem I (PSI) protein and an algal hydrogenase, as well as methods for producing such genetically engineered cells. Also provided herein are methods for increasing hydrogen (H.sub.2) production in cells.

Provided herein are eukaryotic microorganisms having a simple lipid profile comprising long chain fatty acids (LCFAs). Also provided are compositions and cultures comprising the eukaryotic microorganisms as well as methods of using the eukaryotic microorganisms.

The invention is directed to Haematococcus sp. KAU-01 as well as to a culture medium for Haematococcus sp. KAU-01, and to methods for using this strain to process environmental pollutants such as gases generated by coal-fired plants.

The present invention provides methods for making microalgal strains with improved properties relative to the strains from which they are derived. In illustrative embodiments, the methods are performed to produce microalgal strains adapted for use in the industrial production of microalgae-derived biomass products, including but not limited to triglycerides and fatty acids. Also provided are microalgal strains, which can be obtained using the methods described herein, as wells microalgal-derived biomass products, which can be produced from such microalgal strains.

The present invention relates to recombinant cyanobacterial cells for the production of a chemical compound of interest. In particular, the present invention relates to genetic modifications that introduce one or more heterologous phosphopantetheinyl transferases (PPTases) into a cyanobacterial cell. These cells can, optionally, further comprise heterologous carrier protein and nucleic acid constructs that provide the cyanobacterial cells with the capability of producing chemicals of interest or compounds of interest, such secondary metabolites polyketides, nonribosomal peptides and their hybrids, the three major families of bioactive natural products, of cyanobacteria and other bacterial phyla, secondary metabolites analogs, and unnatural compounds.