New methods and assays for multiplexed detection of analytes using phosphors that are uniform in morphology, size, and composition based on their unique optical lifetime signatures are described herein. The described assays and methods can be used for imaging or detection of multiple unique chemical or biological markers simultaneously in a single assay readout.

The invention relates to a continuous method for obtaining a crystalline monosaccharide, comprising: continuous crystallization of the monosaccharide in a main crystallizer (10), wherein crystallization by evaporation and/or crystallization by cooling is carried out continuously on a crystal suspension in the main crystallizer in order to allow crystals of the monosaccharide to grow in the crystal suspension; separation of crystals of the monosaccharide out of the crystal suspension to obtain crystalline monosaccharide; continuous formation of a mass of crystallization magma for the main crystallizer (10) in a cascade, wherein the cascade comprises at least one first stage (13) and a final stage (15) connected in series and each stage comprises at least one pre-crystallizer (13A, 15A), wherein, in the at least one pre-crystallizer (13A) of the first stage (13), a solution is seeded with monosaccharide by means of monosaccharide seed crystals in order to obtain a pre-crystallization magma, and a mass of crystallization magma for the downstream stage (14, 15) is formed from the pre-crystallization magma by means of crystallization by cooling and/or crystallization by evaporation, and wherein a solution containing monosaccharide and a mass of crystallization magma from the upstream stage is supplied to the at least one pre-crystallizer (15A, 15B, 15C) of the final stage (15) to obtain a pre-crystallization magma, and in the at least one pre-crystallizer (15A, 15B, 15C) of the final stage (15) a mass of crystallization magma for the main crystallizer (10) is formed from the pre-crystallisation magma by means of crystallization by cooling and/or crystallization by evaporation; the continuous supply of a solution containing the monosaccharide and a mass of crystallization magma from the at least one pre-crystallizer (15A, 15B, 15C) of the final stage (15) of the cascade to the main crystallizer (10) to provide the crystal suspension.

In accordance with the purpose(s) of the present disclosure, as embodied and broadly described herein, the disclosure, in one aspect, relates to compound Bi-poor perovskite crystals, methods for making the same, and ionizing and other electromagnetic radiation detectors constructed using the Bi-poor perovskite crystals. The Bi-poor perovskite crystals can be synthesized using melt-based growth methods and solution-based growth methods and contain no toxic heavy metals such as lead, cadmium, thallium, or mercury. Devices fabricated from the crystals maintain acceptable levels of performance over time. In some aspects, post-growth annealing can be used to improve the properties, including, but not limited to, room temperature resistivity and response to radiation.

A microfluidic chip comprising at least one dialysis crystallisation cell. The cell includes: a substrate made of PMMA; a first level including a tank defined at least partially by the substrate and by an outer wall of the cell, the tank being in fluid communication with a channel for inlet and a channel for outlet of a solution allowing the crystallisation method to be implemented; and a second level including a dialysis chamber defined at least partially by an inner wall of the cell without contact with the substrate and by a dialysis membrane forming an interface between the tank and the dialysis chamber, the inner wall including at least one one-piece portion in which the periphery of the membrane is kept sealed.

A microfluidic chip comprising at least one dialysis crystallisation cell. The cell includes: a substrate made of PMMA; a first level including a tank defined at least partially by the substrate and by an outer wall of the cell, the tank being in fluid communication with a channel for inlet and a channel for outlet of a solution allowing the crystallisation method to be implemented; and a second level including a dialysis chamber defined at least partially by an inner wall of the cell without contact with the substrate and by a dialysis membrane forming an interface between the tank and the dialysis chamber, the inner wall including at least one one-piece portion in which the periphery of the membrane is kept sealed.

A post-synthetic method for stabilizing colloidal crystals programmed from nucleic acid is disclosed herein. In some embodiments, the method relies on Ag.sup.+ ions to stabilize the particle-connecting nucleic acid duplexes within the crystal lattice, essentially transforming them from loosely bound structures to ones with very strong interparticle links. In some embodiments, the nucleic acid is DNA. Such crystals do not dissociate as a function of temperature like normal DNA or DNA-interconnected colloidal crystals, and they can be moved from water to organic media or the solid state, and stay intact. The Ag.sup.+-stabilization of the nucleic acid (e.g., DNA) bonds is accompanied by a nondestructive contraction of the lattice, and both the stabilization and contraction are reversible with the chemical extraction of the Ag.sup.+ ions, e.g., by AgCl precipitation with NaCl.

A post-synthetic method for stabilizing colloidal crystals programmed from nucleic acid is disclosed herein. In some embodiments, the method relies on Ag.sup.+ ions to stabilize the particle-connecting nucleic acid duplexes within the crystal lattice, essentially transforming them from loosely bound structures to ones with very strong interparticle links. In some embodiments, the nucleic acid is DNA. Such crystals do not dissociate as a function of temperature like normal DNA or DNA-interconnected colloidal crystals, and they can be moved from water to organic media or the solid state, and stay intact. The Ag.sup.+-stabilization of the nucleic acid (e.g., DNA) bonds is accompanied by a nondestructive contraction of the lattice, and both the stabilization and contraction are reversible with the chemical extraction of the Ag.sup.+ ions, e.g., by AgCl precipitation with NaCl.

High-purity hydrous D-allose crystals and a method of efficiently obtaining the crystals are provided. To a D-allose-containing solution having a purity of D-allose of at least 80% (g/g) in a solute, in a metastable region in a supersaturated state of 30° C. or less, D-allose seed crystals are added. Then, the temperature of the solution is lowered by 10° C. or more for cooling and crystallization to initially obtain “hydrous D-allose crystals”, and the crystallization water thereof is removed in a specified temperature zone to obtain novel “anhydrous D-allose crystals”.

A device for fabricating a crystalline conversion layer from a growth solution, has a first wall and a substrate defining between them a crystalline growth cavity; a device for inlet/outlet of the solution controlling, over time, at least the supply or extraction of the growth solution to and from the crystalline growth cavity; a heating device creating a temperature profile in the crystalline growth cavity, the substrate or the first wall; the temperature profile controlling a free formation of the crystalline conversion layer over a thickness of greater than 1 micrometer, in a direction mainly transverse to forming face; the whole of the thickness of the crystalline conversion layer being obtained by the free formation of the crystalline conversion layer.

A device for fabricating a crystalline conversion layer from a growth solution, has a first wall and a substrate defining between them a crystalline growth cavity; a device for inlet/outlet of the solution controlling, over time, at least the supply or extraction of the growth solution to and from the crystalline growth cavity; a heating device creating a temperature profile in the crystalline growth cavity, the substrate or the first wall; the temperature profile controlling a free formation of the crystalline conversion layer over a thickness of greater than 1 micrometer, in a direction mainly transverse to forming face; the whole of the thickness of the crystalline conversion layer being obtained by the free formation of the crystalline conversion layer.