Disclosed herein are methods, compositions and systems for analyzing and detecting enzyme activity. For examples, methods, compositions and systems for parallel detection and analysis of enzymatic activities of enzymes in complex biological mixtures are provided.

Embodiments in accordance with the present disclosure include apparatuses, devices, and methods. For example, a method is directed to method exposing immobilized white blood cells from a blood sample to an antigen. And, scanning the immobilized white blood cells and, therefrom, identify and isolate white blood cells from among the immobilized white blood cells that produce an antibody in response to the exposure to the antigen.

Methods and systems for obtaining inhibitors of Nuclear receptor SET Domain containing protein 2 (NSD2) are disclosed where the methods involve designing compounds that resemble the NSD2 transition state.

The present invention relates to the field of microfluidics and in particular to analysing the gene expression of a cell in response to a ligand expressed in the same microfluidic compartment. By barcoding the transcriptome of the cell and of the expression system generating the ligand, the effect of the ligand on the cell expression can be discerned. The invention provides microfluidic compartments and methods for this purpose.

The present invention relates to the field of microfluidics and in particular to analysing the gene expression of a cell in response to a ligand expressed in the same microfluidic compartment. By barcoding the transcriptome of the cell and of the expression system generating the ligand, the effect of the ligand on the cell expression can be discerned. The invention provides microfluidic compartments and methods for this purpose.

The present invention relates to the field of microfluidics and in particular to analysing the gene expression of a cell in response to a ligand expressed in the same microfluidic compartment. By barcoding the transcriptome of the cell and of the expression system generating the ligand, the effect of the ligand on the cell expression can be discerned. The invention provides microfluidic compartments and methods for this purpose.

There is provided a method for identifying protein methylation on arginine and lysine residues. The method comprises obtaining a set of peptides; blocking un-methylated arginine and lysine residues and the free N-terminal amine of peptides in the set of peptides, so that un-methylated peptides are neutralized and only methylated peptides are positively charged at neutral or basic pH; isolating the methylated peptides based on charge; and performing mass spectrometry (MS) analysis on the isolated methylated peptides to detect methylated lysine and arginine residues. Methods provided herein can be used for large scale, high throughput profiling of protein methylation in a cell or tissue.

There is provided a method for identifying protein methylation on arginine and lysine residues. The method comprises obtaining a set of peptides; blocking un-methylated arginine and lysine residues and the free N-terminal amine of peptides in the set of peptides, so that un-methylated peptides are neutralized and only methylated peptides are positively charged at neutral or basic pH; isolating the methylated peptides based on charge; and performing mass spectrometry (MS) analysis on the isolated methylated peptides to detect methylated lysine and arginine residues. Methods provided herein can be used for large scale, high throughput profiling of protein methylation in a cell or tissue.

Disclosed are methods for performing aptamer preselection based on unique geometry and the content of stems or loops of the aptamer, which methods are capable of providing suitable binders and also permit selection of aptamers performed essentially entirely on a chip or other device. Also disclosed are kits for aptamer selection.

Disclosed are methods for performing aptamer preselection based on unique geometry and the content of stems or loops of the aptamer, which methods are capable of providing suitable binders and also permit selection of aptamers performed essentially entirely on a chip or other device. Also disclosed are kits for aptamer selection.