The present disclosure relates to methods of bioremediation of hydrocarbon-contaminated media using Pseudomonas sp. which can efficiently reduce hydrocarbon contamination in soil, sediment or water in a short period of time.

The present invention relates to compositions and methods for the remediation of contaminated solids and liquids. In particular, embodiments of the present invention relate to the bioremediation of solids and liquids by a composition comprising a biocatalyst or mixture of biocatalysts. The present invention also relates to methods for producing the bioremediation compositions and methods for applying the bioremediation compositions to contaminated sites, including treatment, storage, and disposal facilities, as well as various contaminated water sources, such as aquifers and reservoirs.

A method for scavenging aromatic hydrocarbons present in a contaminated substrate includes treating the contaminated substrate with Pseudomonas taoyuanensis S03 deposited in DSMZ under an accession number DSM 32185, the aromatic hydrocarbons are benzene, naphthalene, toluene, ethylbezene or xylene. A method for scavenging crude petroleum or a refined product thereof present in a contaminated substrate includes treating the contaminated substrate with Pseudomonas taoyuanensis S03 deposited in DSMZ under an accession number DSM 32185, such that the crude petroleum or the refined product thereof is emulsified by Pseudomonas taoyuanensis S03.

A method for scavenging aromatic hydrocarbons present in a contaminated substrate includes treating the contaminated substrate with Pseudomonas taoyuanensis S03 deposited in DSMZ under an accession number DSM 32185, the aromatic hydrocarbons are benzene, naphthalene, toluene, ethylbezene or xylene. A method for scavenging crude petroleum or a refined product thereof present in a contaminated substrate includes treating the contaminated substrate with Pseudomonas taoyuanensis S03 deposited in DSMZ under an accession number DSM 32185, such that the crude petroleum or the refined product thereof is emulsified by Pseudomonas taoyuanensis S03.

A method for inhibiting methane production in naturally occurring or induced reducing conditions, thus subsequently resulting into inhibition of the biomethylation process of the heavy metals is disclosed. The disclosed inhibiting composition blocks 3-hydroxy-3-ethylglutaryl coenzyme A (HMG-CoA) reductase, and 8-hydroxy-5-deazaflavin (coenzyme F.sub.420) in the methane production pathway, due to the presence of lovastatin in the red yeast rice. As a result the methanogens are unable to produce enough quantities of methane that will result to the production of methylmetal(loids), which are usually volatile and more toxic than their inorganic counterparts due to increased water solubility and hydrophobicity.

The present invention relates to compositions and methods for the remediation of contaminated solids and liquids. In particular, embodiments of the present invention relate to the bioremediation of solids and liquids by a composition comprising a biocatalyst or mixture of biocatalysts. The present invention also relates to methods for producing the bioremediation compositions and methods for applying the bioremediation compositions to contaminated sites, including treatment, storage, and disposal facilities, as well as various contaminated water sources, such as aquifers and reservoirs.

A wastewater stream is flowed from a separator to an anode side of a microbial electrolysis cell (MEC). The wastewater stream includes water and hydrocarbons. The separator is positioned in a gas-oil separation plant. The MEC electrolyzes the hydrocarbons to produce hydrogen ions. A membrane separates the MEC into the anode side and a cathode side. The membrane allows the hydrogen ions and water molecules to pass through the membrane from the anode side to the cathode side, thereby forming a treated wastewater stream at the cathode side. The MEC combines the hydrogen ions at the cathode side to produce hydrogen gas. The treated wastewater stream and a hydrogen gas stream is discharged from the cathode side. The hydrogen gas stream includes the hydrogen gas produced by the MEC. The hydrogen gas stream is oxidized into water. Electrical power is generated in response to oxidizing the hydrogen gas into water.

A carbon-based aerogel doped or enriched with nitrogen and phosphorus is provided. Further, kits comprising the aerogel and oil-degrading bacteria are provided. Use of the kits for at least partially degrading a crude oil or a crude oil fraction is also provided.

A method of converting waste into energy is provided. The method includes the following steps: (a) providing a lipid-containing substrate to react with a biocatalyst to produce a surfactant molecular liquid; (b) pretreating an organic waste with the surfactant molecular liquid to produce a first organic liquid; (c) subjecting the first organic liquid to an ultrasonic treatment to produce a second organic liquid; and (d) subjecting the second organic liquid to an anaerobic biological treatment for conversion to methane. The biocatalyst includes at least one lipase. Moreover, the surfactant molecular liquid includes at least one of monoglyceride and diglyceride. A waste treatment system for converting waste into energy is also provided.

A method for the bioremediation of hydrocarbon-contaminated production water through the microbiological process of culturing salt-tolerant bacteria in a high-salt cell culture medium for decontamination. The high-salt cell culture medium contains sodium chloride, sodium sulfate, sodium bicarbonate, calcium chloride, and calcium chloride, along with agar, water, and salt-tolerant bacteria to form broth. Once incubated to form an inoculation mixture, this broth is combined with production water to produce a bacteria-containing liquid mixture, and then treated with production water, bioremediating by propagating the bacteria-containing liquid mixture in the treated production water.