The present invention relates to new strains of Bacillus paralicheniformis with improved growth rates and plant growth promoting properties and new strains of Bacillus paralicheniformis with improved growth rates when compared to their parental strains from where they are derived.

The present invention relates to new strains of Bacillus paralicheniformis with improved growth rates and plant growth promoting properties and new strains of Bacillus paralicheniformis with improved growth rates when compared to their parental strains from where they are derived.

The present invention relates to a Bacillus subtilis JCK-1398 strain (Accession No. KCTC 14084BP) having induced resistance activity in pine trees and various plants, a pesticidal or antibacterial composition comprising same as an active ingredient, a composition for controlling plant diseases or pests, and a control method using same. The Bacillus subtilis JCK-1398 strain of the present invention was experimentally confirmed to have control activity against pests, nematodes, and fungi, which cause various plant diseases, by inducing resistance in a host. Thus, the Bacillus subtilis JCK-1398 strain of the present invention can be effectively used to control related plant diseases and can be spread over a wide area through foliar spraying, and therefore is expected to be able to prevent the spread of pine wilt disease at low cost.

The present invention provides a recombinant Bacillus subtilis JY011802 that can produce sublancin in a high yield, which was deposited at the China General Microbiological Culture Collection Center on Oct. 31, 2018 with an accession number of CGMCC No. 16667, and an application thereof. The yield of the sublancin produced by the recombinant Bacillus subtilis can reach 3100 mg/L.

The present invention provides a recombinant Bacillus subtilis JY011802 that can produce sublancin in a high yield, which was deposited at the China General Microbiological Culture Collection Center on Oct. 31, 2018 with an accession number of CGMCC No. 16667, and an application thereof. The yield of the sublancin produced by the recombinant Bacillus subtilis can reach 3100 mg/L.

Methods for controlling plant pests are disclosed. In particular, insecticidal proteins having toxicity to Spodoptera insect pests are provided. Polynucleotides comprising codons optimized for expression in plants encoding the insecticidal proteins are also provided. Methods of making the insecticidal proteins and methods of using the insecticidal proteins and polynucleotides encoding the insecticidal proteins of the invention, for example in transgenic plants to confer protection from insect damage, are also disclosed.

Provided is a method for intracellularly producing a large amount of a Cry protein in a Bacillus bacterium. A method for producing a Cry protein or a culture product comprising the Cry protein, comprising transforming a Bacillus bacterium with an expression plasmid incorporating a gene encoding the Cry protein operably linked to a regulatory region comprising a σA-dependent promoter or a σH-dependent promoter, and culturing the transformed cell, wherein the expression plasmid comprises a polynucleotide encoding a replication protein consisting of the amino acid sequence set forth in SEQ ID NO: 9 or a protein having an identity of 80% or more or more with the amino acid sequence of the replication protein and involved in replication initiation.

Provided is a method for intracellularly producing a large amount of a Cry protein in a Bacillus bacterium. A method for producing a Cry protein or a culture product comprising the Cry protein, comprising transforming a Bacillus bacterium with an expression plasmid incorporating a gene encoding the Cry protein operably linked to a regulatory region comprising a σA-dependent promoter or a σH-dependent promoter, and culturing the transformed cell, wherein the expression plasmid comprises a polynucleotide encoding a replication protein consisting of the amino acid sequence set forth in SEQ ID NO: 9 or a protein having an identity of 80% or more or more with the amino acid sequence of the replication protein and involved in replication initiation.

Provided are biologically pure bacterial isolates characterized by a genome structure at least 90% similar to a genome structure of a bacterial species selected from the group consisting of: Streptomyces sp. E128 having an NRRL Accession No. B-67462, Bacillus amyloliquefaciens A190 having an NRRL Accession No. B-67464, Bacillus subtilis P243 having an NRRL Accession No. B-67459, Bacillus thuringiensis M979 having an NRRL Accession No. B-67457, Massilia aurea P63 having an NRRL Accession No. B-67461, Rhodococcus sp. G706, Stenotrophomonas maltophilia E132 having an NRRL Accession No. B-67460, Streptomyces aurantiacus A918, Streptomyces badius 0180, Streptomyces mirabilis B670 having an NRRL Accession No. B67463, Streptomyces scopuliridis F427 having an NRRL Accession No. B-67458, and Streptomyces sp. L219. Also provided are whole cell broth or lysates thereof, and polynucleotide, polypeptides and constructs expressing same, compositions-of-matter comprising same and methods using same for killing or inhibiting the development of insects.

Provided are biologically pure bacterial isolates characterized by a genome structure at least 90% similar to a genome structure of a bacterial species selected from the group consisting of: Streptomyces sp. E128 having an NRRL Accession No. B-67462, Bacillus amyloliquefaciens A190 having an NRRL Accession No. B-67464, Bacillus subtilis P243 having an NRRL Accession No. B-67459, Bacillus thuringiensis M979 having an NRRL Accession No. B-67457, Massilia aurea P63 having an NRRL Accession No. B-67461, Rhodococcus sp. G706, Stenotrophomonas maltophilia E132 having an NRRL Accession No. B-67460, Streptomyces aurantiacus A918, Streptomyces badius 0180, Streptomyces mirabilis B670 having an NRRL Accession No. B67463, Streptomyces scopuliridis F427 having an NRRL Accession No. B-67458, and Streptomyces sp. L219. Also provided are whole cell broth or lysates thereof, and polynucleotide, polypeptides and constructs expressing same, compositions-of-matter comprising same and methods using same for killing or inhibiting the development of insects.