Compositions, methods, and systems are provided for fluorescent polymerase enzyme substrates comprising protein shields for improving enzyme photostability in single molecule real time sequencing. Fluorescent polymerase enzyme substrates of the invention have a protein shield between the fluorescent dye moieties and nucleotide moieties of the polymerase enzyme substrate. The polymerase enzyme substrates have a nucleotide component and a dye component, each attached to a protein. The attachments can be covalent. The protein can, for example, prevent the direct interaction of the fluorescent dye moiety with the enzyme when carrying out nucleotide synthesis, preventing photodamage to the enzyme. The polymerase enzyme substrates of the invention can have multiple dyes and multiple nucleotide moieties.

The present invention provides a protein fusion construct comprising a far-red cyanobacteriochrome (CBCR) domain linked to a heterologous domain, wherein the far-red CBCR domain comprises a CBCR polypeptide and a tetrapyrrole chromophore. The invention also provides nucleic acids, expression cassettes, vectors, and host cells for expression of the far-red CBCR protein fusion constructs. Methods for detecting cellular components, methods for imaging biological structures, and method for modulating cellular processes using the protein fusion constructs are also provided.

The present invention provides a protein fusion construct comprising a far-red cyanobacteriochrome (CBCR) domain linked to a heterologous domain, wherein the far-red CBCR domain comprises a CBCR polypeptide and a tetrapyrrole chromophore. The invention also provides nucleic acids, expression cassettes, vectors, and host cells for expression of the far-red CBCR protein fusion constructs. Methods for detecting cellular components, methods for imaging biological structures, and method for modulating cellular processes using the protein fusion constructs are also provided.

In some aspects, the disclosure relates to production of bacterial secondary metabolites. In some embodiments, the disclosure relates to a genetically engineered Streptomyces J1074 bacterium, wherein the bacterium comprises a nucleic acid having a modification to at least one global regulator gene.

In some aspects, the disclosure relates to production of bacterial secondary metabolites. In some embodiments, the disclosure relates to a genetically engineered Streptomyces J1074 bacterium, wherein the bacterium comprises a nucleic acid having a modification to at least one global regulator gene.

This document provides methods and materials involved in treating cancer. For example, methods and materials for using a BiPE that can include (a) one or more molecules having the ability to bind to a cancer cell (e.g., a human breast cancer cell), (b) an optional linker component, and (c) one or more molecules having the ability to bind to an antigen presenting cell (e.g., a human macrophage) to treat cancer are provided.

This document provides methods and materials involved in treating cancer. For example, methods and materials for using a BiPE that can include (a) one or more molecules having the ability to bind to a cancer cell (e.g., a human breast cancer cell), (b) an optional linker component, and (c) one or more molecules having the ability to bind to an antigen presenting cell (e.g., a human macrophage) to treat cancer are provided.

A peptide cyclase that has the amino acid sequence represented by SEQ ID NO: 1 or a mutated sequence thereof, or a peptide cyclase that has an amino acid sequence encoded by a base sequence encoding the amino acid sequence represented by SEQ ID NO: 1 or a mutated sequence thereof; DNA encoding the peptide cyclase; a method for producing the peptide cyclase; and a method for producing a cyclic peptide using the peptide cyclase.

A peptide cyclase that has the amino acid sequence represented by SEQ ID NO: 1 or a mutated sequence thereof, or a peptide cyclase that has an amino acid sequence encoded by a base sequence encoding the amino acid sequence represented by SEQ ID NO: 1 or a mutated sequence thereof; DNA encoding the peptide cyclase; a method for producing the peptide cyclase; and a method for producing a cyclic peptide using the peptide cyclase.

The present invention relates to a monomeric streptavidin-expressing gene construct and a host cell into which a recombinant vector comprising the gene construct has been introduced. The gene construct according to the present invention, after injected in vivo through a strain, may express streptavidin, thereby making it possible not only to monitor in real time the location of the strain or a cancer tissue pre-targeted by the strain by using a biotinylated diagnostic agent, but also to increase the cancer targeting efficiency of a biotinylated anticancer agent.