Compositions and methods are directed to engineered extracellular matrix protein—mussel foot protein fusions for use as a bioadhesive for repairing tissues. The compositions have one or more of: (i) at least one hydrophobic region; (ii) at least one crosslinking region; (iii) at least one tyrosine residue accessible to be enzymatically modified to a DOPA or TOPA side chain; (iv) at least one mussel foot protein; (v) at least one mussel foot protein loop; (vi) at least one human extracellular protein loop; or (vii) at least one of the following sequences: SEQ ID NO: 1, SEQ ID NO: 2, SEQ ID NO: 3, SEQ ID NO: 4, SEQ ID NO: 5, or SEQ ID NO: 6. The elastin-like polypeptide includes at least one non-naturally occurring amino acid or sequence alteration.

Conjugates comprising a targeting moiety specific for the CXCR4 and based on the polyphemusin-derived peptide and a therapeutic or imaging agent are provided. Therapeutic and diagnostic methods with the conjugates which require specific targeting to CXCR4+cells are provided as well.

A cell culture substrate including a fibrous web in which fibers are integrated, and a cell culture coating layer comprising a coating film connecting at least some fibers from among fibers positioned on one surface of the fibrous web, wherein the cell culture coating layer is realized through a fusion protein for cell culture in which a functional peptide is bound to a mussel adhesive protein. Thus, the substrate can be stored at room temperature for a long period of time, i.e., several years, despite containing protein-like substances that aid in cell culture, and thus exhibits very high storage stability. At the same time, the activity of substances that aid in cell culture is maintained at the same level or is reduced only minimally, thus enabling cell culture at an initially designed level.

Embodiments of the present disclosure generally relate to methods and compositions for stabilizing biological material using intrinsically disordered proteins. In an embodiment, a composition is provided, the composition including a first component comprising at least one intrinsically disordered protein; and a second component comprising at least one biological material of interest, at least one biologically-derived material of interest, or both, the second component being free of the at least one intrinsically disordered protein. The methods and compositions include at least one intrinsically disordered protein that can be modified to prevent, or at least mitigate, polymerization thereof and the formation of gel-like matrices, thereby, e.g., improving the ability of the intrinsically disordered proteins to protect and stabilize sensitive biological materials.

Provided herein are alpha-conotoxin peptide analogs, including alpha-conotoxin peptide analogs that are covalently attached to polyethylene glycol (PEG), and pharmaceutical compositions of such alpha-conotoxin peptide analogs. Also provided herein are methods of treating or preventing a condition conducive to treatment or prevention by inhibition of an α9-containing nicotinic acetylcholine receptor (nAChR) (e.g., the α9α10 subtype of the nAChR) in a subject.

The invention belongs to the field of biotechnology, in particular, relates to peptide fragment, monoclonal antibody, colloidal gold test strip and detection method thereof used for detection of stichopus oligopeptide. Sequences of the peptide fragments are KIVPGVPD and GRDGDQGPV. After identification, the peptides KIVPGVPD and GRDGDQGPV are newly discovered oligopeptides, which are exclusive peptide fragments of Stichopus oligopeptide.

New insecticidal proteins, nucleotides, peptides, their expression in plants, methods of producing the peptides, new processes, production techniques, new peptides, new formulations, and new organisms, a process which increases the insecticidal peptide production yield from yeast expression systems. The present invention is also related and discloses selected endotoxins we call cysteine rich insecticidal peptides (CRIPS) which are peptides derived from Bacillus thuringiensis (Bt) and their genes and endotoxins in combination with toxic peptides known as Inhibitor Cystine Knot (ICK) genes and peptides as well as with other types of insecticidal peptides such as trypsin modulating oostatic factor (TMOF) peptide sequences used in various formulations and combinations; of both genes and peptides, useful for the control of insects.

A diagnostically useful carrier includes a means for specifically capturing an antibody to a polypeptide from the group including squid MLC1 or squid MLC2 or a variant thereof in a sample from a subject. A method includes the step detecting in a sample from a subject the presence or absence of an antibody to squid MLC1 or squid MLC2. The polypeptide or the carrier or a polypeptide binding specifically to an IgE antibody from the sample of a patient to squid MLC1 or squid MLC2 are useful for the manufacture of a diagnostic kit, preferably for the diagnosis of allergy.

A voltage indicator includes a polypeptide sequence comprising a voltage-sensitive opsin domain and a capture protein domain arranged and disposed to capture a fluorescent dye ligand. When the fluorescent dye ligand is captured and the voltage indicator is bound to a cell membrane, an increase in voltage across the cell membrane causes an increase in fluorescent emission.

The disclosure provides a method of generating a sterile fish, crustacean, or mollusk. The method comprises breeding (i) a fertile hemizygous mutated female fish, crustacean, or mollusk with (ii) a fertile hemizygous mutated male fish, crustacean, or mollusk, selecting a female progenitor that is homozygous by genotypic selection, and breeding the homozygous female progenitor to produce the sterile fish, crustacean, or mollusk. The mutation disrupts the maternal-effect of a primordial germ cell (PGC) development gene and does not impair the viability, sex determination, fertility, or a combination thereof, of a homozygous progenitor. The disclosure also provides methods of making broodstock freshwater and seawater organisms for use in producing sterilized freshwater and seawater organisms, as well as the broodstock itself.