Anti-CD33 antibodies and antigen-binding fragments thereof and anti-CD33/anti-CD3 bispecific antibodies or antigen-binding fragments thereof are described. Also described are nucleic acids encoding the antibodies, compositions comprising the antibodies, methods of producing the antibodies, and methods of using the antibodies for treating or preventing diseases, such as cancer.

The present invention is directed to heterodimeric antibodies that bind CD3 and PSMA.

The present disclosure relates to compositions and methods of engineered IgG Fc constructs, wherein the Fc constructs include one or more Fc domains.

The present invention relates to an anti-CD47/anti-CTLA-4 bispecific antibody and a preparation method thereof and an application thereof. The bispecific antibody comprises (a) a first antigen-binding portion, comprising a heavy chain variable region (V.sub.H) and a light chain variable region (V.sub.L), where V.sub.H and V.sub.L form an antigen-binding site that specifically binds to CD47; and (b) a second antigen-binding portion comprising a single domain antibody (sdAb) that specifically binds to CTLA-4, the first antigen-binding portion and the second antigen-binding portion are fused to each other. The bispecific antibody to which the present invention relates may simultaneously block two means of tumor immune escape, and therefore has a better effect in tumor immunotherapy.

The present invention relates to a bispecific protein and a method preparing the same, wherein mutation is introduced into heavy chains and/or light chains to enhance heterodimerization between a heavy chain (CH3 domain or Fc) and a heavy chain (CH3 domain or Fc) and dimerization between a heavy chain (CH1 domain) and a light chain, both targeting the same material, thereby constructing heterodimeric bispecific proteins of high purity. A bispecific protein according to the present invention can find applications in a variety of fields comprising cancer therapy, singling regulation, diagnosis, etc.

The present invention provides stable liquid pharmaceutical formulations comprising a human bispecific antibody that specifically binds to human MUC16 and human CD3. In certain embodiments, the formulations contain, in addition to the bispecific antibody, a buffer, a surfactant, and a sugar. The pharmaceutical formulations of the present invention exhibit a substantial degree of antibody stability upon stress and storage.

A tetravalent, homodimer-type bispecific antibody molecule that simultaneously targets immune effector cell antigen CD3 and human epidermal growth factor receptor 2 (Her2); the bispecific antibody molecule comprises, from in sequence from N-terminus to C-terminus, a first single-chain Fv capable of specifically binding to Her2, a second single-chain Fv capable of specifically binding to CD3, and an Fc fragment; the first and second single-chain Fv are connected by means of a connection peptide, and the second single-chain Fv is connected to the Fc directly fragment or is connected by means of a connection peptide; the Fc fragment does not have effector functions such as CDC, ADCC and ADCP. The bispecific antibody may significantly inhibit or kill tumor cells, and has controlled toxic side effects that may be caused by excessive activation of effector cells. The maximum safe starting dose in preclinical toxicology evaluation tests is significantly higher than other doses having the same target, and no systemic immunotoxicity occurs, suggesting that the drug administration safety window for the bispecific antibody is wide; in addition, said bispecific antibody is a homodimer that does not experience the problem of heavy chain and light chain mismatching; the steps of purification are simple and efficient, expression is high, and the physicochemical and in vivo stability of the antibody are significantly improved.

This disclosure provides a monoclonal population of highly sialylated multimeric binding molecules where the population includes IgM antibodies, IgM-like antibodies, or other IgM-derived binding molecules, where the population of binding molecules has a higher level of sialic acid content than is found in normal serum IgM. Also provided are methods of producing such monoclonal populations of highly sialylated multimeric binding molecules.

This invention relates to anti-TIM-3 antibodies and antibody compositions and their use in enhancing immunity in a patient, e.g., to treat cancer.

The present disclosure provides an analysis method for measuring the content of light chain-exchanged molecules in a composition containing a multi-specific antigen-binding molecule. The analysis method of the present disclosure includes the steps of: treating a composition comprising a multi-specific antigen-binding molecule and preparing a plurality of types of F(ab) fragments; and measuring the F(ab) fragments by a separation method based on electric charge or hydrophobic interactions and determining the content (content ratio) of each fragment.