A method of bioprinting a bioink printed structure and a bioprinted composition resulting from this method is provided. Biological cells are mixed with biomaterial inks. These biomaterial inks have a biopolymer backbone with grafted thereto a first bio-orthogonal chemical group. The mixed biological cells and biomaterial inks are extruded into a support bath. The next step is diffusing crosslinking molecules which have a second (complementary to the first) bio-orthogonal chemical group, in the support bath, whereby the diffusing crosslinking molecules react via bioorthogonal click-chemistry between the first and second bio-orthogonal chemical groups resulting in covalently crosslinking the biomaterial ink into a printed structure. Embodiments of this invention can be directed towards personalized medicine and printed tissue engineering constructs, as well as drug discovery by printing complex tissue mimics or printing model vasculature for studying cardiovascular disease.

Compositions for and methods of digitally printing an ink image onto a woven textile material are provided. A base application is applied to the surface of a woven textile material, especially one comprising synthetic resin fibers prior to application of an ink image layer. The base application comprises an acrylic latex material that is formulated to be printed on with a digital ink while still wet, thereby eliminating the need for an intermediate drying cycle in between base application deposit and printing of the ink image.

Compositions for and methods of digitally printing an ink image onto a woven textile material are provided. A base application is applied to the surface of a woven textile material, especially one comprising synthetic resin fibers prior to application of an ink image layer. The base application comprises an acrylic latex material that is formulated to be printed on with a digital ink while still wet, thereby eliminating the need for an intermediate drying cycle in between base application deposit and printing of the ink image.

3D printing of biopolymer-based inks provides for manufacturing a broad range of products with desirable properties. A print nozzle may be charged to form a cone-shaped ink droplet to result in increased resolution, more reliable contact with irregular surfaces, and a mechanism to control contacting the ink to the print surface.

Silkworm silk, has been processed into a variety of new material formats including, hydrogels, ultrathin films, thick films, conformal coatings, 3D porous or solid matrices, fibers and many related material formats. Silk is processed in an all water-based, room temperature, neutral pH environment, is mechanically stable, edible, and biocompatible. Given the favorable material properties, the use of silk-based inks have been used in a variety of controlled chemical and biological material fabrication on the micro- and nano-scale. Tuning of the properties of silk-based inks has led to the utilization of more diverse printing techniques and has expanded the scale and function of the printed substrates.

Silkworm silk, has been processed into a variety of new material formats including, hydrogels, ultrathin films, thick films, conformal coatings, 3D porous or solid matrices, fibers and many related material formats. Silk is processed in an all water-based, room temperature, neutral pH environment, is mechanically stable, edible, and biocompatible. Given the favorable material properties, the use of silk-based inks have been used in a variety of controlled chemical and biological material fabrication on the micro- and nano-scale. Tuning of the properties of silk-based inks has led to the utilization of more diverse printing techniques and has expanded the scale and function of the printed substrates.

A bio-ink composition comprises a plurality of bio-block, in which the bio-blocks can serve as basic building blocks in cell-based bioprinting. The bio-blocks, pharmaceutical compositions comprising the bio-blocks, methods of preparing artificial tissues, tissue progenitors, or multi-dimensional constructs, and methods of preparing the bio-blocks are also provided. The bio-blocks, and the multi-dimensional constructs, artificial tissues, and tissue progenitors comprising the bio-blocks or prepared by the methods described herein are useful for tissue engineering, in vitro research, stem cell differentiation, in vivo research, drug screening, drug discovery, tissue regeneration, and regenerative medicine.

A bio-ink composition comprises a plurality of bio-block, in which the bio-blocks can serve as basic building blocks in cell-based bioprinting. The bio-blocks, pharmaceutical compositions comprising the bio-blocks, methods of preparing artificial tissues, tissue progenitors, or multi-dimensional constructs, and methods of preparing the bio-blocks are also provided. The bio-blocks, and the multi-dimensional constructs, artificial tissues, and tissue progenitors comprising the bio-blocks or prepared by the methods described herein are useful for tissue engineering, in vitro research, stem cell differentiation, in vivo research, drug screening, drug discovery, tissue regeneration, and regenerative medicine.

Provided is a bioink set for printing a construct that is able to carry cells, including a bioink which contains a biodegradable polyurethane and a biopolymer, and a divalent metal ion solution. The biopolymer is gelatin, agar, alginate salts, hyaluronic acid and salts thereof, chitosan, and any combination thereof. Also provided are a method of preparing a construct for carrying cells by three-dimensional printing with the bioink set, and a method of three-dimensional printing of cells by using an ink composition.

Provided is a bioink set for printing a construct that is able to carry cells, including a bioink which contains a biodegradable polyurethane and a biopolymer, and a divalent metal ion solution. The biopolymer is gelatin, agar, alginate salts, hyaluronic acid and salts thereof, chitosan, and any combination thereof. Also provided are a method of preparing a construct for carrying cells by three-dimensional printing with the bioink set, and a method of three-dimensional printing of cells by using an ink composition.