The present invention relates to a bioink for 3D printing, the preparation method, and the usage. Such bioink is a gel made of α-zein, porogen by 0-10% of the weight of zein, ethanol and water. The preparation method consists of: 10-50% zein is dissolved into an aqueous solution containing 40-90% (v/v) of ethanol, then, the porogen by 0-10% of the weight of zein is added, and then, this solution is allowed to stand at 5-95° C. for 1-10 days, or stirred for 30 min-24 hours, and thus, the bioink for 3D printing can be obtained. The conditions applied to prepare such bioink are mild and the method adopted is easy to operate, in addition, the said bioink for 3D printing has good mechanical properties and biocompatibility, which can be applied in the field of biomedicine for preparing tissue engineered substitutes and hemostatic materials by 3D printing at room temperature.

The present invention relates to a bioink for 3D printing, the preparation method, and the usage. Such bioink is a gel made of α-zein, porogen by 0-10% of the weight of zein, ethanol and water. The preparation method consists of: 10-50% zein is dissolved into an aqueous solution containing 40-90% (v/v) of ethanol, then, the porogen by 0-10% of the weight of zein is added, and then, this solution is allowed to stand at 5-95° C. for 1-10 days, or stirred for 30 min-24 hours, and thus, the bioink for 3D printing can be obtained. The conditions applied to prepare such bioink are mild and the method adopted is easy to operate, in addition, the said bioink for 3D printing has good mechanical properties and biocompatibility, which can be applied in the field of biomedicine for preparing tissue engineered substitutes and hemostatic materials by 3D printing at room temperature.

Cellulose-based composite material comprising an electrically conductive material dispersed in a matrix comprising at least one plant-derived protein and a polymer of aleuritic acid, said composite material being obtainable by a process comprising the steps of dissolving at least one plant-derived protein and aleuritic acid in a dissolving solution to achieve a first mixture, dispersing an electrically conductive material in said first mixture to achieve a conductive ink, distributing said conductive ink on at least one side of a cellulose substrate to achieve a coated cellulose substrate, hot-pressing said coated cellulose substrate to obtain i) impregnation of the cellulose substrate with said conductive ink and ii) polymerization of aleuritic acid.

Cellulose-based composite material comprising an electrically conductive material dispersed in a matrix comprising at least one plant-derived protein and a polymer of aleuritic acid, said composite material being obtainable by a process comprising the steps of dissolving at least one plant-derived protein and aleuritic acid in a dissolving solution to achieve a first mixture, dispersing an electrically conductive material in said first mixture to achieve a conductive ink, distributing said conductive ink on at least one side of a cellulose substrate to achieve a coated cellulose substrate, hot-pressing said coated cellulose substrate to obtain i) impregnation of the cellulose substrate with said conductive ink and ii) polymerization of aleuritic acid.

A bio-ink composition comprises a plurality of bio-block, in which the bio-blocks can serve as basic building blocks in cell-based bioprinting. The bio-blocks, pharmaceutical compositions comprising the bio-blocks, methods of preparing artificial tissues, tissue progenitors, or multi-dimensional constructs, and methods of preparing the bio-blocks are also provided. The bio-blocks, and the multi-dimensional constructs, artificial tissues, and tissue progenitors comprising the bio-blocks or prepared by the methods described herein are useful for tissue engineering, in vitro research, stem cell differentiation, in vivo research, drug screening, drug discovery, tissue regeneration, and regenerative medicine.

A bio-ink composition comprises a plurality of bio-block, in which the bio-blocks can serve as basic building blocks in cell-based bioprinting. The bio-blocks, pharmaceutical compositions comprising the bio-blocks, methods of preparing artificial tissues, tissue progenitors, or multi-dimensional constructs, and methods of preparing the bio-blocks are also provided. The bio-blocks, and the multi-dimensional constructs, artificial tissues, and tissue progenitors comprising the bio-blocks or prepared by the methods described herein are useful for tissue engineering, in vitro research, stem cell differentiation, in vivo research, drug screening, drug discovery, tissue regeneration, and regenerative medicine.

Provided are a method of preparing atelocollagen and use thereof, more specifically, a method of removing immunogens from pig skin-derived collagen and preparing high-purity atelocollagen with high yield, and use thereof. Atelocollagen prepared with high yield, according to a preparation method of the present disclosure, has high purity, a low immune response, no cytotoxicity, excellent in vivo biodegradability, and high safety, and therefore, may be used in a form of a bio-ink capable of forming high-resolution structures with excellent morphological characteristics and division ability, and therefore, is expected to be used for the development of a product for tissue and regeneration engineering, including a cell scaffold capable of easily forming blood vessels and controlling drug delivery.

Provided are a method of preparing atelocollagen and use thereof, more specifically, a method of removing immunogens from pig skin-derived collagen and preparing high-purity atelocollagen with high yield, and use thereof. Atelocollagen prepared with high yield, according to a preparation method of the present disclosure, has high purity, a low immune response, no cytotoxicity, excellent in vivo biodegradability, and high safety, and therefore, may be used in a form of a bio-ink capable of forming high-resolution structures with excellent morphological characteristics and division ability, and therefore, is expected to be used for the development of a product for tissue and regeneration engineering, including a cell scaffold capable of easily forming blood vessels and controlling drug delivery.

The invention is directed to methods and compositions for inkjet assisted synthesis of a plurality of polynucleotides at reaction sites on a substrate using template-free polymerases, such as, terminal deoxynucleotidyl transferases (TdTs). Compositions of the invention include formulations of synthesis reagents for inkjet delivery including, but not limited to, TdT coupling reaction buffers and 3′-O-protected dNTP monomers.

The invention is directed to methods and compositions for inkjet assisted synthesis of a plurality of polynucleotides at reaction sites on a substrate using template-free polymerases, such as, terminal deoxynucleotidyl transferases (TdTs). Compositions of the invention include formulations of synthesis reagents for inkjet delivery including, but not limited to, TdT coupling reaction buffers and 3′-O-protected dNTP monomers.