A system and method using a subterranean biological reactor can include a pre-reactor storage unit configured to receive a feedstock including a slurry of biologically derived material and at least one pump configured to pump the effluent from the pre-reactor storage unit. The system may include at least one wellbore containing a subterranean biological reactor configured to receive the effluent from the pre-reactor storage unit. At least a portion of the subterranean biological reactor may be configured to perform anaerobic digestion upon the effluent to generate a biogas.

A system and method using a subterranean biological reactor can include a pre-reactor storage unit configured to receive a feedstock including a slurry of biologically derived material and at least one pump configured to pump the effluent from the pre-reactor storage unit. The system may include at least one wellbore containing a subterranean biological reactor configured to receive the effluent from the pre-reactor storage unit. At least a portion of the subterranean biological reactor may be configured to perform anaerobic digestion upon the effluent to generate a biogas.

A method for recovering subsurface fluid from rock formations by adding an enzyme-based green solvent to a carrier fluid and injecting the enzyme-based green solvent and the carrier fluid into a production well. The enzyme-based green solvent and the carrier fluid are directed down a flow path of the production well. The solvent soaks in the production well before reversing the flow path of the production well. The enzyme-based green solvent and the carrier fluid travel up the flow path with a plurality of recovered deposits from the production well in the enzyme-based green solvent.

The subject invention provides compositions comprising solvents and surfactants, as well as their use in improving oil and/or gas production. In some embodiments, the compositions comprise chemical or synthetic solvents and/or surfactants. In other embodiments, the compositions comprise biological components, such as microorganisms and/or their growth by-products. The subject invention can be used to dissolve, disperse and/or emulsify paraffin precipitates and/or deposits; prevent and/or inhibit paraffin deposition; remove rust deposits and prevent corrosion associated therewith; inhibit bacterial growth and/or biofilm formation; and to enhance oil recovery.

The subject invention provides compositions comprising solvents and surfactants, as well as their use in improving oil and/or gas production. In some embodiments, the compositions comprise chemical or synthetic solvents and/or surfactants. In other embodiments, the compositions comprise biological components, such as microorganisms and/or their growth by-products. The subject invention can be used to dissolve, disperse and/or emulsify paraffin precipitates and/or deposits; prevent and/or inhibit paraffin deposition; remove rust deposits and prevent corrosion associated therewith; inhibit bacterial growth and/or biofilm formation; and to enhance oil recovery.

A method and a system for recovering oil from currently inaccessible oil containing geological units by activating the deep biosphere microbial seed bank. Nutrient and thermal enhancement of microorganisms in oil containing geological units allows for stimulation of inactive and/or dormant microorganisms such that they proliferate and produce gas. The oil viscosity that is decreased by heat, along with the gas pressure produced by activated microbes which allows previously inaccessible oil to flow toward production wells.

A method and a system for recovering oil from currently inaccessible oil containing geological units by activating the deep biosphere microbial seed bank. Nutrient and thermal enhancement of microorganisms in oil containing geological units allows for stimulation of inactive and/or dormant microorganisms such that they proliferate and produce gas. The oil viscosity that is decreased by heat, along with the gas pressure produced by activated microbes which allows previously inaccessible oil to flow toward production wells.

A method of treating a subterranean formation, the method including placing a well treatment fluid comprised of at least an enzyme and a breaker additive in the subterranean formation. Initially, the pH of the well treatment fluid is about 11.5. The breaker additive reduces the pH of the well treatment fluid by at least 1.5 to increase the activity of enzyme and accelerate hydrolysis of a crosslinkable component.

A method of treating a subterranean formation, the method including placing a well treatment fluid comprised of at least an enzyme and a breaker additive in the subterranean formation. Initially, the pH of the well treatment fluid is about 11.5. The breaker additive reduces the pH of the well treatment fluid by at least 1.5 to increase the activity of enzyme and accelerate hydrolysis of a crosslinkable component.

A nutrient composition for enhancing biogenic methane production from a carbonaceous material is described. The nutrient composition comprises a source of phosphorus (P) and a source of nitrogen (N), wherein the molar ratio of phosphorus to nitrogen (P/N) is greater than 1.5, and the nitrogen concentration is at least 0.1 m M and less than 1.7 m M. A process for enhancing biogenic methane production from a carbonaceous material is also described. The process involves contacting the nutrient composition of the invention with the carbonaceous material for a period of time to biogenically produce methane and subsequently collecting methane from the carbonaceous material. The process may further comprise contacting the carbonaceous material with a second nutrient composition, wherein the second nutrient composition has a P/N molar ratio greater than the P/N molar ratio of the former nutrient composition.