When detachment of a cell sheet from a culture dish has started at an unintended timing, immediately detach the cell sheet from the culture dish while preventing generation of wrinkles and the like in the cell sheet. A cell culture device captures an image of cells in the culture dish, and controls a cooling mechanism and a shaking mechanism based on the image so as to shake the cells while cooling them.

The present invention provides a new gene mutation assessment system which makes it possible, even when a gene mutation has considered to be apparently not associated with a trait from mutation information at a single position, to pick the gene mutation as a gene mutation candidate showing an association with the trait. The gene mutation assessment device (10) of the present invention includes a communication unit (19) that is capable of communicating a database DB, an assessment target mutation information acquisition unit (11) that acquires mutation information of a common gene mutation in a sample group showing a common trait as mutation information of an assessment target mutation, a score assignment unit (12) that assigns a first score showing an association with a trait of the DB information to the assessment target mutation based on the DB information, a score determination unit (13) that compares the first score with an association threshold and determines the assessment target mutation as a re-scoring target when the first score is less than the association threshold, a region mutation information acquisition unit (14) that acquires, as region mutation information, a gene mutation in an associated region with respect to a re-scoring target assessment target mutation based on the DB information, a score re-assignment unit (15) that assigns a second score weighted to the first score to the re-scoring target assessment target mutation based on the region mutation information, and an assessment score determination unit (16) that determines the second score as an assessment score of the re-scoring target assessment target mutation.

A temperature control device has a temperature control target for holding, in the inside thereof, a DNA-containing solution, the temperature of which is to be adjusted with a single temperature control unit, a first heat transfer unit which is in contact with the temperature control unit and transfers heat between the temperature control unit and a temperature control target. A second heat transfer unit is in contact with the first heat transfer unit and the temperature control target and transfers heat between the first heat transfer unit and the temperature control target or is in contact with the temperature control unit and the temperature control target and transfers heat between the temperature control unit and the temperature control target, and in that the temperature control target is sandwiched by the first heat transfer unit and the second heat transfer unit, and the second heat transfer unit is pressed.

According to one embodiment of the present invention, a multiplex PCR device is disclosed. The multiplex PCR device comprises a multiplex PCR chip simultaneously carrying a plurality of mutually different nucleic acid molecules, and the invention may be characterised in that, attached spaced apart from each other on the multiplex PCR chip, there are a plurality of probes used for hybridization reactions whereby hybridization takes place specifically with mutually different amplified sequences of the nucleic acid molecules.

According to one embodiment of the present invention, a multiplex PCR device is disclosed. The multiplex PCR device comprises a multiplex PCR chip simultaneously carrying a plurality of mutually different nucleic acid molecules, and the invention may be characterised in that, attached spaced apart from each other on the multiplex PCR chip, there are a plurality of probes used for hybridization reactions whereby hybridization takes place specifically with mutually different amplified sequences of the nucleic acid molecules.

Used for running a polymerase chain reaction, a thermal cycler includes a main body having a chamber, and inside of the chamber is provided with a protocol running device port and a sample block for placing reaction tubes. When a protocol running device is put into the protocol running device port, the main body may execute a thermal cycle onto the reaction tubes according to a protocol stored in the protocol running device.

The present disclosure provides a culture device and a preparation method for a cell membrane, and relates to the field of cell culture technology. The culture device for a cell membrane includes a semiconductor refrigerator, and one or more culture vessels configured to culture a cell membrane. The semiconductor refrigerator includes a first insulating substrate, a second insulating substrate, and at least one semiconductor thermocouple disposed between the first insulating substrate and the second insulating substrate. The one or more culture vessels are disposed on a side of the first insulating substrate away from the second insulating substrate.

The present disclosure provides a culture device and a preparation method for a cell membrane, and relates to the field of cell culture technology. The culture device for a cell membrane includes a semiconductor refrigerator, and one or more culture vessels configured to culture a cell membrane. The semiconductor refrigerator includes a first insulating substrate, a second insulating substrate, and at least one semiconductor thermocouple disposed between the first insulating substrate and the second insulating substrate. The one or more culture vessels are disposed on a side of the first insulating substrate away from the second insulating substrate.

The present invention provides facile and accurate molecular diagnosis of disease-specific genes capable of the naked eye detection through amplifying the target genes to selectively block the fluid path in a microfluidic device. Specifically, the present invention includes an isothermal amplification of target genes through a rolling circle amplification, a microfluidic device for detecting pathogen genes, and a detection method using the same. Therefore, the present invention can conveniently detect a single target gene, such as a single pathogen, or at the same time, several target genes, such as several pathogens, without complicated mechanical equipment.

The present invention provides facile and accurate molecular diagnosis of disease-specific genes capable of the naked eye detection through amplifying the target genes to selectively block the fluid path in a microfluidic device. Specifically, the present invention includes an isothermal amplification of target genes through a rolling circle amplification, a microfluidic device for detecting pathogen genes, and a detection method using the same. Therefore, the present invention can conveniently detect a single target gene, such as a single pathogen, or at the same time, several target genes, such as several pathogens, without complicated mechanical equipment.