A device for in-vitro modelling in-vivo tissues of organs that includes a device for in-vitro modelling in-vivo tissues of organs, the device including an open access chamber, a perfusion channel, and a culturing membrane dividing the access chamber from the perfusion channel, wherein the culturing membrane is porous and flexible and/or elastic.

A cell culture matrix is provided that has a substrate with a first side, a second side opposite the first side, a thickness separating the first side and the second side, and a plurality of openings formed in the substrate and passing through the thickness of the substrate. The plurality of openings allow flow of at least one of cell culture media, cells, or cell products through the thickness of the substrate, and provides a uniform, efficient, and scalable matrix for cell seeding, proliferation, and culturing. The substrate can be formed from a woven polymer mesh material that provides a high surface area to volume ratio for cells and good fluid flow through the matrix. Bioreactor systems incorporating the cell culture matrix and related methods are also provided.

A system for controlling the development of a culture in solid medium in a robotised incubator (100), having an imaging system (1) configured to take images without moving a Petri dish (5) from the location in which the culture is carried out, and a system for analysing the images obtained by the imaging system, wherein, on the basis of the information provided by the system for analysing the images from the imaging system, which analyses the images provided by the imaging system by a growth detection algorithm, the development of the culture in an incubator (100) is determined, without moving the Petri dish (5) from the location in which the culture is carried out.

A culture apparatus includes a fine water generating cartridge disposed in an air passage by which moisture is supplied into a culture space and that goes into a moisture absorption state in which moisture in air is adsorbed on conductive polymer films by reducing the temperature of a base material having the conductive polymer films, and goes into a moisture release state in which fine water with a particle size of 50 nanometers or less is released from the moisture adsorbed on the conductive polymer films by increasing the temperature of the base material; and a control part performing moisture absorption control bringing the fine water generating cartridge into the moisture absorption state, and moisture release control releasing the fine water by bringing the fine water generating cartridge into the moisture release state supplied into the culture space, by irradiating the subject with the fine water.

The present invention provides means for producing a parathyroid gland organoid. A culture method of the present invention includes a cell population containing a parathyroid gland cell in a ratio of 50% or more, and a mesenchymal cell in a ratio of 10% or more; and a step of culturing the cell population.

A packed-bed bioreactor system is provided, the system including a cell culture vessel having a first end, a second end, and a reservoir between the first and second ends; and a cell culture matrix disposed in the reservoir. The cell culture matrix includes a structurally defined substrate with a plurality of interwoven fibers having surfaces for adhering cells thereto. The substrate is disposed within the reservoir in a wound configuration creating a plurality of layers of substrate in the wound configuration, and none of the plurality of layers of substrate are separated by a spacer material.

Provided is a cell culture scaffold material having excellent extensibility of pseudopodia and excellent cell proliferation. The cell culture scaffold material according to the present invention contains a peptide-conjugated polyvinyl alcohol derivative having a polyvinyl alcohol derivative portion and a peptide portion, and has a hydroxyl value of 1,100 mgKOH/g or less.

A spheroid cell culture article including: a frame having a chamber including: an opaque side wall surface; a top aperture; a gas-permeable, transparent bottom; and optionally a chamber annex surface and second bottom, and at least a portion of the transparent bottom includes at least one concave arcuate surface, is disclosed. Methods of making and using the article are also disclosed.

A cell culture well insert may include a frame defining a first open end, a second open end, and at least one support extending therebetween. The cell culture well insert may also include a fluid permeable mesh coupled to the frame and disposed across the second open end. The mesh may define pores that have an average pore size in a range from 10 micrometers to 100 micrometers.

A cell culture matrix is provided that has a substrate with a first side, a second side opposite the first side, a thickness separating the first side and the second side, and a plurality of openings formed in the substrate and passing through the thickness of the substrate. The plurality of openings allow flow of at least one of cell culture media, cells, or cell products through the thickness of the substrate, and provides a uniform, efficient, and scalable matrix for cell seeding, proliferation, and culturing. The substrate can be formed from a woven polymer mesh material that provides a high surface area to volume ratio for cells and good fluid flow through the matrix. Bioreactor systems incorporating the cell culture matrix and related methods are also provided.