The invention relates to a microbioreactor assembly (01) having a plurality of microbioreactors. The microbioreactor assembly comprises a microtiter plate (02) having numerous wells (09) in a predefined grid arrangement, a closed bottom surface (14) and an open upper face. An insert unit (03) is also provided, which is arranged on the upper face of the microtiter plate (02) and has numerous inserts (11) in the same grid arrangement, each insert (11) engaging in a well (09), and the well (09) being divided into at least two regions (12, 17). Finally, the microbioreactor assembly comprises an activation unit (04), which is placed on the insert unit (03) and has numerous pumps (26), each of which is connected to supply channels (19), which allow the transport of fluid between the two regions (12, 17).

Disclosed herein is a bioreactor system that allows perfusive flow through a porous support medium enabling 3D growth of biological samples. In some embodiments, the system comprises a sample well filled with a three-dimensional (3D) cell growth medium. The system can further comprises a liquid medium reservoir fluidly connected to the sample well by a first filter material. The system can further comprises a medium collection chamber fluidly connected to the sample well by a second filter material. The system can further comprise an absorbant material that creates an osmotic pressure gradient to produce perfusive flow. In some embodiments, osmotic pressure draws fluid from the liquid medium reservoir, through the first filter material, into the sample well where it permeates the three-dimensional cell growth medium, through the second filter material, and finally into the medium collection chamber.

A cell cultivation apparatus for cultivating microorganisms and growing cells at high density is provided. The apparatus includes a membrane comprising multiple surface features on a first side of the membrane for cell placement. The surface features comprising one or more compartments within which a cell can be located. The membrane includes a material that is at least partially permeable to gas. A second side of the membrane defines a gas region. The second side of the membrane is separated from the first side of the membrane by the membrane. The apparatus further includes a media region for receiving media. The compartments are configured to at least partially reduce media flow shear forces on one or more cells in the compartments. The surface features may be ridges, protrusions, fins, wells, and/or posts.

A method for producing a cell culture insert with at least one membrane, in particular at least one biological membrane, may include the following steps: providing at least one insert blank with at least one opening; and forming the at least one membrane in the insert blank by means of a bio-printing method.

The methods and systems described herein provide a cell culture platform with an array of tissue modeling environments and dynamic control of fluid flow. The cell culture platform includes an array of wells that are fluidically coupled by microchannel structures. The dynamically controlled flow of fluid interacts with cells grown within the microchannels.

Described herein is a cell culture device and methods of use in three-dimensional cell co-cultures and for use in studying paracrine signaling in vitro.

A Micro-Well Array Plates (MWAP) assembly for high throughput microfluidic devices for studying cells and method of manufacturing thereof are provided. The MWAP assembly includes a top plate having a plurality of macro-wells arranged in an array within a frame. The MWAP assembly also includes a bottom plate operable to be secured to the bottom surface of the frame, the bottom plate having a plurality of arrays of micro-wells. The MWAP assembly includes a well grid formed when the bottom plate is secured to the top plate via the plurality of macro-wells and the plurality of arrays micro-wells. The well grid with the plurality of macro-wells and the plurality of arrays micro-wells enable visualization of cells via a high throughput.

The invention relates to a cell culture carrier for cultivating biological cell material (07). The cell culture carrier comprises: a carrier plate (01); a membrane (02), which is supported by the carrier plate (01) and provides a colonization surface (06) for the cell material (07), the colonization surface (06) being permeable for a main flow (10) of a nutrient solution; and a holding cage (03), which is covered by the membrane (02) at an end and into which the cell material (07) can be introduced and through which the main flow (10) can flow. The carrier plate (01) provides a plurality of flow openings (08), which are positioned outside of the holding cage (03) in peripheral distribution and which allow a secondary flow (11) of the nutrient solution, the flow velocity of which secondary flow is greater than the flow velocity of the main flow (10) of the nutrient solution that flows through the holding cage (03) and the colonization surface (06). The invention further relates to a cell culture carrier assembly having a cell culture carrier of this type in a housing, which provides a flow region for a nutrient solution.

A fluidic device for culturing cells includes a microplate and plate lid. The microplate includes multiple wells and channels, the channels extending between the wells such that the channels interconnect the wells. The plate lid releasably engages the microplate to thereby enclose the wells and the channels The wells include a culture surface such that a cell culture medium received therein is deposited over the culture surface. At least one channel that extends between adjacent ones of the wells is spaced from the culture surfaces of the adjacent wells defining a gap between the at least one channel and the culture surfaces of the adjacent wells for collection of the cell culture medium.

A cell culture vessel including an accommodating part which is an inner space accommodating a cell culture support therein, a fixing member configured to fix the cell culture support to a lower surface of the accommodating part, wherein the fixing member includes a first adhesive layer attached to the lower surface of the accommodating part, a second adhesive layer attached to a lower surface of the cell culture support, and a support film interposed between the first adhesive layer and the second adhesive layer and configured to perform a support function, and an adhesive force between the second adhesive layer and the cell culture support is higher than an adhesive force between the first adhesive layer and the lower surface of the accommodating part.