The present disclosure relates to a method for producing cultured meat, the method including: forming a cell sheet by culturing cells usable for producing cultured meat; and culturing the cells in a form in which a nanofilm is formed on a surface of the cell sheet by coating the cell sheet. The present disclosure provides a method for producing cultured meat that implements excellent mechanical strength by protecting a cell layer from external stress and performing stable cell proliferation, and provides cultured meat implementing quality and taste that are improved compared to those of conventional cultured meat by reproducing tissue similar to muscle tissue of an actual animal.

The present disclosure provides a biomimetic cell culture apparatus that mimics interactions among organs in a human body. The present disclosure includes a plurality of culture units for culturing cells, a conduit for connecting the plurality of culture units to each other to form a circulating path, a pump unit disposed on the conduit for forming a flow in culture medium such that the culture medium circulates through the plurality of culture units, and an agitating module for agitating the plurality of culture units.

A multiwell instrument includes a vessel having an inside; a plurality of culture plates arranged in the inside of the vessel, each individual one of the plurality of culture plates having an inside in which material to be examined can be stored; and filters disposed inside the respective culture plates to partition the insides of the respective culture plates into at least two wells which are horizontally adjacent.

A cell analysis system includes a multi-layer microfluidic device that includes a layer of microfluidic channels, a layer of microwells, a membrane with nanochannels, and a layer of extraction chambers. The microwells and the membrane are configured to allow culturing of cells that are adhered to the membrane or suspended in the microwells, and the membrane is configured to allow diffusion of substances across the membrane into the layer of extraction chambers. The cell analysis system includes a top conductive layer and a bottom conductive layer on the opposite sides of the multi-layer microfluidic device. The cell analysis system also includes a function generator configured to apply an electroporation pulse between the top conductive layer and the bottom conductive layer.

Provided are a method for producing a hepatocyte culture and a method for improving or maintaining a function of a hepatocyte or a hepatic progenitor cell, each method including a culturing step of culturing a hepatocyte or a hepatic progenitor cell inside at least a part of a plurality of opening pores and communication pores of a porous film having the plurality of opening pores provided on a surface thereof and the communication pores for communicating the opening pores adjacent to each other; and a cell-containing porous film.

The present invention provides a pigmentation skin model that comprises: a first cell group containing fibroblasts damaged by light irradiation, said first cell group being seeded on a first cell culture substratum; and a second cell group containing melanocytes and keratinocytes, said second cell group being applied onto the first cell group. The present invention also provides a method for producing the pigmentation skin model, and a method for evaluating a factor for treating or preventing pigmentation of the skin, said method comprising using the pigmentation skin model.

Methods and systems for cell media exchange wherein media may be aspirated without also aspirating cells and wherein removal and re-plating of cells is not necessarily required. For example, the cell culture system or apparatus features a gap that is small enough to retain cells therein and also sized to prevent media from leaking. The methods and systems of the present invention may help reduce stress and damage to cells as compared to traditional methods that require removing and re-plating cells.

3D cell cultures and devices for 3D cell culture, and methods of use thereof are provided. In some embodiments, the 3D cell culture comprise pancreatic β cells and can be generated in multi-well plates, allowing for high throughput assays on the cell culture.

Disclosed is a permeable membrane support having an attachable and detachable permeable membrane, the permeable membrane support comprising: a lower support; an upper support partially accommodated into the lower support; and a permeable membrane disposed between the lower support and the upper support. When the upper support and the lower support are engaged with each other in a snap-fit manner, the permeable membrane is sandwiched between a bottom opening of a cylindrical lower member of the upper support and a top face of a bottom support portion of the lower support.

The present disclosure describes systems and methods for providing culturing of a number of various tissue types in an air-liquid configuration in a high-throughput format and allowing co-culture of cells as well as application of physiologically relevant flow. A microfluidic cell culturing device is provided that includes a first channel having a first inlet port and a second inlet port, the first channel defined in a first layer. The microfluidic cell culturing device includes a membrane layer having a first surface coupled to the first layer defining the first channel, the membrane layer comprising semipermeable membrane that forms at least a portion of a surface of the first channel. The microfluidic cell culturing device includes a chamber defined in a second layer that exposes a portion the membrane layer to an external environment, wherein the chamber overlaps a portion of the first channel across the membrane layer.