Patent classifications
C12M27/06
BIOREACTOR FOR PRODUCTION AND HARVESTING OF MICROALGAE
A bioreactor (30) for production and harvesting of microalgae is described, comprising a reactor basin (32) in the form of a tank arranged to receive CO2 and water as well as algae, and which is equipped with at least one outlet for harvesting of algal biomass, where the reactor basin (32) comprises a rotating beam (54) equipped with one or more tillable mixing grates (60) and harvesting grates (62).
COMPLETE YOGURT MAKER APPLIANCE
A yogurt maker device comprises an outer shell to house a yogurt vessel that receives milk and a culture container that receives yogurt culture. A processor within the outer shell controls a heating element to boil milk within the yogurt vessel using a temperature sensor as feedback and for maintaining a predefined boiling temperature for a predefined period of boiling time and a stirring device to continuously stir boiling milk within the yogurt vessel while boiling and also while cooling to a cooling predefined temperature until fermentation temperature is reached. The processor also controls a culture container base holding the culture container and having a motor to automatically pour the yogurt culture when the fermentation temperature is reached. Finally, a cooling mechanism automatically provides cooling to contents of the yogurt vessel once fermentation is complete.
Systems for cell culture scaling
The present set of embodiments relate to a bioproduction system, method, and apparatus for creating a scalable bioreactor system. Specifically, the present set of embodiments enable the determination of bioreaction performance characteristics of a commercial scale by matching operational parameters between a small test scale bioreaction to that of a commercial scale bioreaction. The system and methods do not rely on simply making bioreactor apparatuses across scales the same dimensionally which would not account for differences in fluid dynamic properties between very small to very large volumes, but requires tuning of a variety of systems (mixing assembly, sparger system, and headspace airflow system) in conjunction with one another to achieve predictive outcomes.
Conversion of lignocellulosic biomass into biogas
A method for biogas production includes feeding a receiving structure with a feed containing biomass that comprises lignocellulose and directing the feed from the receiving structure into an anaerobic secretome bioreactor (ASB) reactor environment which includes a synthetic microbial community consisting of at least one selected from extremophile thermophilic anaerobic microorganisms that are essentially acidogens and acetogens, the synthetic microbial community producing a secretome of exozymes. The ASB treated biomass is further directed to an aerobic digestion (AD) reactor environment, the ASB treated biomass being pasteurized so as to be essentially free of non-thermophilic microorganisms, the ASB treated biomass comprising liquid effluent containing solubilized biomass products and metabolized biomass products, and solid effluent. Contents or heat are recycled through a conduit between the ASB reactor environment and the AD reactor environment.
Continuous Fermenter for Sequential Fermentation of Hexose-Pentose
Discloses is a continuous fermenter for sequential fermentation of hexose and pentose which includes (a) a hexose fermenter equipped with a saccharified solution supply unit containing hexose, pentose and lignin, a plurality of trays closing at least half of the diameter of the fermenter, impellers disposed on each of the trays, an impeller driving unit, a lignin discharge unit disposed at the bottom of the fermenter, a fermented solution discharge unit, and a temperature control jacket; and (b) a pentose fermenter equipped with a fermented solution supply unit for supplying the fermented solution discharged from the hexose fermenter, a plurality of trays closing at least half of the diameter of the fermenter, impellers disposed on each of the trays, an impeller driving unit, a lignin discharge unit disposed at the bottom of the fermenter, a fermented solution discharge unit, and a temperature control jacket.
Method and apparatus for the use of micro-carriers in a disposable bioreactor system
A system and method for compartmentalizing micro-carriers in a bioreactor includes a container configured to store micro-carriers and a vessel configured to contain a fluid so as to prevent the micro-carriers from entering the vessel during sterilization, shipping, storage, or other pre-use handling of the system. One or more addition lines connect the container and the vessel such that the vessel is in fluid communication with the vessel. The one or more addition lines are contactable by at least one blocking element configured to reversibly block fluid flow between the container and the vessel. The container, addition lines, and vessel are configured to allow the micro-carriers to be injected into the vessel at any point during a cell culture run. The vessel may also include a rotatable wheel, a harvest port configured to allow for the removal of the micro-carriers from the vessel, and a media removal port comprising a retention screen for removing spent medium.
SYSTEM AND METHOD FOR THERMOPHILIC ANAEROBIC DIGESTER PROCESS
An anaerobic digestion system is provided that includes a blend tank operable to control and perform pre-treatment of feedstock. An anaerobic digester is operable to digest the feedstock provided from the blend tank in a totally enclosed oxygen-free environment within a specific temperature range. A bio-mass tank processes liquid digestate from the anaerobic digester. One or more baffles are positioned in the digester, with the one or more baffles providing for plug flow through at least a portion of the digester to create baffled zones that are at least partially operable independently of adjacent baffled zones. A bio-mass tank processes liquid digestate from the anaerobic digester. An energy source is coupled to the anaerobic digester.
METHOD FOR DIGESTING BIODEGRADABLE PACKAGING WASTE AND AN APPARATUS THEREOF
The present invention relates to a method for digesting biodegradable packaging waste and an apparatus thereof. Specifically, the present invention relates to a method of treating biodegradable packaging waste prior to subjecting the biodegradable packaging waste to a biological valorisation process and an apparatus thereof. The apparatus includes a tank for receiving the biodegradable packaging waste therein, the tank comprises a top portion and a bottom portion below the top portion, an air supply module adapted to supply air into the tank, a microbe injector adapted to inject mesophilic microbe into the tank, such that the tank is adapted to receive the biodegradable packaging waste, air and mesophilic microbe therein, wherein the biodegradable packaging waste is subjected to mesophilic aerobic digestion prior to conveying the biodegradable packaging waste from the tank to a biological valorisation system to subject the biodegradable packaging waste to the biological valorisation process.
CONVERSION OF FEEDSTOCKS INTO BIOGAS
A method and system for processing biomass comprising lignocellulosic materials (LM), fats, oils, and grease (FOG), and/or waste-activated sludge (WAS) for biogas production. Biomass is maintained in an anaerobic secretome bioreactor with a synthetic microbial community under thermophilic conditions within a specified pH range. The microbial community produces a secretome of exozymessuch as cellulases, hemicellulases, ligninases, lipases, and proteasesthat hydrolyze and solubilize portions of the LM, FOG, and WAS, resulting in a liquid effluent containing sugars, amino acids, fatty acid anions, and other intermediates. The thermophilic environment also pasteurizes the effluent, which is subsequently processed in a mesophilic methanogenic digestion stage for biogas production. In this stage, volatile fatty acid anions are further metabolized to yield methane (CH.sub.4), carbon dioxide (CO.sub.2), and bicarbonate ions (HCO.sub.3.sup.) as primary products of methanogenesis.
Trap-type carbon replenishing device for culturing microalgae of opened pool and carbon replenishing method thereof
The invention relates to the field of microalgae culture and specifically relates to a trap-type carbon supplement device and carbon supplement method for cultivating microalgae in an open pond. The trap-type carbon supplement device for cultivating microalgae in an open pond, comprises a trap-type container, a partition plate and a gas distributor, wherein the gas distributor is positioned at the culture solution inlet of the trap-type carbon supplement device; the thickness of the trap-type carbon supplement device on the side of the culture solution inlet is 0.5-2 times of the depth of the culture solution in the open pond; the gap between the lower end of the partition plate and the bottom of the trap-type container is 0.5-2 times of the thickness of the trap-type carbon supplement device on the side of the culture solution inlet; the upper end of the partition plate is higher than the wall of the trap-type container; and the width of the partition plate is matched with the trap-type container. The carbon supplement device of the invention can make the gas-liquid contact time longer and reduce the depth of the trap-type container, therefore it can reduce the flow resistance of the liquid in the carbon supplement device and save energy consumption.