Bioreactor systems can include a first frame and a second frame, a well plate, a motor plate, a motor, and a controller. The first frame may define a well plate inset and standoff insets for a first set of metal standoffs. The second frame may define a plurality of mounts and a plurality of insets for a second set of metal standoffs. A gear may be positioned in each of the plurality of mounts. A paddle may be coupled to each of the gears. The well plate can be positioned within the well plate inset. The motor plate can be supported by and connected to the first set of metal standoffs and the second set of metal standoffs. The motor can be mounted on the motor plate and operatively connected to one of the plurality of gears.

A method for optimizing a process (PROC) to produce a biochemical product (P) defined by a quality attribute, the process being controlled by an actuation parameter (C) and being monitored to get a measured value (T). The method includes training a predictive model (PRED) on a training database; and deploying the trained predictive model (PRED) to provide a correction actuation parameter (dC) when a predicted quality attribute (pQA) is out of a targeted quality attribute interval (QAmin, QAmax). The method also includes a step of designing a physical model of the process (PROC) able to provide a simulated quality attribute, the training database comprising simulated quality attributes computed from the physical model and experimental quality attributes computed from biochemical products (P) previously produced.

A bioreactor support system configured to hold a bioreactor bag, comprising: a base module comprising an impeller drive unit; and at least two vessel units of different sizes, which are configured to support and substantially enclose a side wall of a bioreactor bag when a bioreactor bag is provided in the bioreactor support system, wherein the base module and the vessel units comprise mating connection devices such that the vessel units can be connected to one and the same base module and such that the impeller drive unit of the base module can be used to drive an impeller in a bioreactor bag when a bioreactor bag is provided in the bioreactor support system.

A bioproduction mixing system includes a flexible compartment having a first end, a second end, and a sidewall extending therebetween, a center axis passing through the flexible compartment between the first end and the second end. A helical assembly is disposed within the flexible compartment and is suspended between the first end and the second end, the helical assembly being offset from the center axis of the flexible compartment.

Bioreactors including bioreactors having features for improved performance, sensing and ease of use. Many embodiments provide bioreactors having improved dip-tubes, magnetically coupled agitators, condensers and sensing ability. Particular embodiments provide a bioreactor including a vessel having an inner volume for liquid contents and a head plate (HP) for coupling a plurality of components to the bioreactor where the HP is coupled to a top portion of the vessel and includes a plurality of ports. A diametric magnetic (DM) drive assembly (DMDA) and agitation shaft (AS) including at least one impeller are rotatably coupled to the HP. The DMDA and AS are rotated by non-vertical magnetic forces from a rotating DM positioned above the HP. A dip tube assembly (DTA) having a plurality of inner fluidic channels is positioned through a HP port such that a DTA end extends into the vessel for delivery liquids and sparging gasses.

There is provided a method for producing a product by cells, the method including filtering a cell broth through a porous membrane to obtain a product by the cells, in which the porous membrane has a liquid contact surface having at least any one selected from the group consisting of an opening ratio of 57.5% or less, a pore diameter having a coefficient of variation of 0.5 or less, and a ratio of pore diameter/fiber diameter of 1.3 or less, and the liquid contact surface has at least any one selected from the group consisting of a structural anisotropy of 0.97 or more and 1.03 or less and a ratio of long pore diameter/short pore diameter of 1.8 or less, and a shear stress due to a flow of the cell broth on the liquid contact surface is set to 4.0 N/m.sup.2 or less.

There is provided a method for producing a product by cells, the method including filtering a cell broth through a porous membrane to obtain a product by the cells, in which the porous membrane has a liquid contact surface having at least any one selected from the group consisting of an opening ratio of 57.5% or less, a pore diameter having a coefficient of variation of 0.5 or less, and a ratio of pore diameter/fiber diameter of 1.3 or less, and the liquid contact surface has at least any one selected from the group consisting of a structural anisotropy of 0.97 or more and 1.03 or less and a ratio of long pore diameter/short pore diameter of 1.8 or less, and a shear stress due to a flow of the cell broth on the liquid contact surface is set to 4.0 N/m.sup.2 or less.

A method of installing a flexible bioprocess container in a bioprocessing system having a rigid housing with an interior compartment and extending between a top and an opposing base. The method includes coupling a first surface of the flexible bioprocess container to a moveable platform positioned within the interior compartment of the rigid housing; coupling a second surface of the flexible bioprocess container to the base of the rigid housing; and moving the movable platform relative to the rigid housing and toward the top of the rigid housing so as to at least partially expand the flexible bioprocess container within the interior compartment of the rigid housing.

The present invention relates to a bioreactor for cell culture. According to an embodiment of the present invention, vibration is provided to a cell culture solution (solution) to facilitate cell separation, and, since the vibration is transmitted to the entire cell culture solution without dead zones, cell separation efficiency is excellent.

The disclosure relates to an impeller system (1) for use with a bioreactor (2), having a drive shaft (3) configured for being rotated in a rotational direction (R) around a longitudinal axis (X) of the drive shaft by a drive motor (4) of the bioreactor; at least two impeller blades (5) connected to the drive shaft, configured for being rotated along with the drive shaft when the drive shaft is rotated, wherein the at least two impeller blades are configured for transitioning from a collapsed state to an un-collapsed state, for performing agitation. At least one of the at least two impeller blades transitions from the collapsed state to the un-collapsed state by rotating along a circumference (6) of the drive shaft due to resistance from a liquid (7) in the bioreactor when agitation is performed.