Disclosed herein are compositions comprising an isolated cellulose degrading fungus and pharmaceutical substances produced by the fungus.

A device includes an input chamber, an attractant chamber, a migration channel arranged in fluid communication between an outlet of the input chamber and inlet of the attractant chamber, a baffle arranged in fluid communication between the outlet of the input chamber and the migration channel or within the migration channel, and an exit channel in fluid communication with the migration channel at a point beyond the baffle and before the migration channel enters the inlet of the attractant chamber. The baffle is configured to inhibit movement of a first type of cell through the baffle to a greater extent than the baffle inhibits movement of a second type of cell through the baffle.

A cell culture cartridge is provided comprising a plurality of zones geometrically configured to provide for symmetrical fluid flow with each of the plurality of zones to avoid dead areas in flow within each of the plurality of zones. In certain embodiments, at least eight inlets are provided, with an inlet positioned at each corner of the cell culture cartridge. In certain embodiments, a shared outlet is positioned on a top surface of the cell culture cartridge.

A cell culture cartridge is provided comprising a plurality of zones geometrically configured to provide for symmetrical fluid flow with each of the plurality of zones to avoid dead areas in flow within each of the plurality of zones. In certain embodiments, at least eight inlets are provided, with an inlet positioned at each corner of the cell culture cartridge. In certain embodiments, a shared outlet is positioned on a top surface of the cell culture cartridge.

A method for culturing a microbial or cellular seed culture includes positioning a first flexible bag within a chamber of a first retainer, the first retainer being secured to a mixer table. A culture is produced within a compartment of the flexible bag, the culture comprising a growth media and a microbial or cellular starter culture added thereto. The mixer table is activated so that the culture is mixed within the compartment of the first flexible bag while the microbes or cells of the culture grow, the culture having a maximum volume of less than 10 liters.

Algae cultivation systems and methods account for weather variations that can affect algae cultivation. In one system, an open raceway algae cultivation system includes a channel having a high section and a low liquid collection section. The channel is sloped to allow substantially all of an algae cultivation fluid in the high section to flow downwardly into the low liquid collection section. A barrier is removably positioned in the high section and a drain is positioned in the high section such that, when substantially all of the algae cultivation fluid has collected in the low liquid collection section, any rainwater that falls in the high section flows into the drain, without the rainwater mixing with the algae cultivation fluid in the low liquid collection section.

Provided herein are systems and methods for culturing tissue-like assemblies of cells in the presence of a pulsating alternating ionic magnetic resonance field. The cells are introduced into a growth module in fluid connection with a nutrient module in which the gravity vector of the growth module is continually randomized and cultured in the presence of the alternating ionic magnetic resonance field.

Provided are devices and methods for producing microbe-based compositions that can be used in the oil and gas industry, environmental cleanup, as well as for other applications. The devices and methods can produce scalable, submerged yeast cultures for inoculating larger-scale, on-site fermentation systems. A device can include a rotatable drum mounted on a support frame and a motor connected to the drum and causing the drum to rotate.

The present disclosure relates to a system including a main exterior body. The main exterior body includes (i) a return section at a bottom of the main exterior body, (ii) a gas disengagement section at a top of the main exterior body, (iii) a riser section positioned between the return section and the gas disengagement section, and (iv) a downcomer section positioned between the gas disengagement section and the return section. The system also includes one or more first spargers each having a first end and a second end opposite the first end. Each of the one or more first spargers are positioned such that the first end is outside of the main exterior body and the second end is positioned in a lower portion of the riser section of the main exterior body. The one or more first spargers are configured to supply compressed gas to the riser section of the main exterior body. The system also includes one or more second spargers each having a first end and a second end opposite the first end. Each of the one or more second spargers are positioned such that the first end is outside of the main exterior body and the second end is positioned in a lower portion of the downcomer section of the main exterior body adjacent to the return section. The one or more second spargers are configured to supply compressed gas to the downcomer section of the main exterior body.

A flow electrotransfection device with a chamber for fluid to flow though, a liquid inlet channel for communicating with the chamber and a liquid outlet channel for communicating with the chamber, includes an electrode module for applying an electric field to fluid in the chamber, and further includes a fluid guiding structure for making flow velocities of fluid flowing through the central zone and the peripheral zone of the chamber tend to be consistent. The present disclosure solves the technical problem of relatively uneven the flow velocities of fluid at different zones in the flow electrotransfection chamber, improves the accuracy of the number of electric shock when the fluid flows though the chamber, thus improving the electrotransfection efficiency, and reduces the cell damage and even death from excessive times of electric shock, thereby improving cell viability.