The present disclosure provides system (10) and a method for algal cultivation. The system for algal cultivation comprises a reservoir (1) which is configured to contain a fluid medium (6) having an algal culture; at least one circulating means (3) which is configured to circulate the fluid medium in the reservoir at a desired velocity; at least one covering means (5) which is configured in its operative configuration to at least partially cover an opening of the reservoir to at least partially block the light incident on the fluid medium and define intermittent light and dark cycle for the fluid medium for a predetermined period of time in the reservoir. Advantageously, the invention disclosed by the present disclosure improves biomass production, minimizes water evaporation in open pond system, avoids photo-inhibition or stress, provides optimum photosynthetic rate, provides efficient utilization of the space, provides efficient generation of resources such as renewable energy.

Systems and methods relating to dynamic spargers for generating fine bubbles within reactors such as biological and chemical reactors. A sparger system is positioned within a reactor and comprises a support plate, multiple annular shrouds engaged with the support plate, and spargers positioned within the annular shrouds defining a gap between an interior surface of the annular shroud and an exterior surface of the corresponding sparger. Liquid flows through the defined gap between an interior surface of the annular shroud and an exterior surface of the sparger. Acceleration of the liquid through the gap shears bubbles at the exterior surface of the sparger creating bubbles or fine bubbles.

A cell activation reactor and a cell activation method are provided. The cell activation reactor includes a body, a rotating part, an upper cover, a microporous film, and multiple baffles. The body has an accommodating space, which is suitable for accommodating multiple cells and multiple magnetic beads. The rotating part is disposed in the accommodating space and includes multiple impellers. The microporous film is disposed in the accommodating space and covers multiple holes of the accommodating space. The baffles are disposed in the body. When the rotating part is driven to rotate, the interaction between the baffles and the impellers separates the cells and the magnetic beads.

A cell culture cartridge is provided comprising a plurality of zones geometrically configured to provide for symmetrical fluid flow with each of the plurality of zones to avoid dead areas in flow within each of the plurality of zones. In certain embodiments, at least eight inlets are provided, with an inlet positioned at each corner of the cell culture cartridge. In certain embodiments, a shared outlet is positioned on a top surface of the cell culture cartridge.

Devices and methods cell culture are disclosed herein. In particular embodiments, the cell culture devices include multi-channel devices with a non-linear flow path that recapitulates the three-dimensional microarchitecture and physiological organ-level functions of human organs with cellular and molecular resolution.

A method of transfecting a population of cells, comprising the steps of treating the population of cells to disrupt a cell membrane of at least some of the cells, and introducing foreign material into at least some of the cells through the disrupted cell membranes. The cell membranes are disrupted by passing a cell-containing carrier liquid along a microfluidic channel having a cross-sectional dimension greater a cross-sectional dimension of the cells and comprising a flow obstacle configured to abruptly change a flow vector of the cells and/or carrier liquid in the microfluidic channel from a first direction to a second direction. The abrupt change in flow vector of the cells is configured to cause transfection-competent disruption of the cell membrane of at least some of the cells due to impact of the cells with the flow obstacle and/or with other cells.

A fermentation tank includes a tank body, enclosing a cavity therein, having a material inlet and a material outlet; a heat exchange structure, disposed on a wall of the cavity for heat exchanging with the tank body; at least one flow disturbing plate, being an elongated plate, the width direction of the flow disturbing plate being parallel to a radial direction of the tank body, the longitudinal direction of the flow disturbing plate being parallel to a vertical direction, a length of the flow disturbing plate being larger than a width thereof, the flow disturbing plate being arranged in the cavity and connected to the tank body, the flow disturbing plate having a heat exchange channel therein, two ends of the heat exchange channel communicating an exterior respectively so that heat is exchanged between the at least one flow disturbing plate and the cavity.

Photobioreactor devices and units for the production of biomass and remediation of environmental contamination are provided. The bioreactor devices comprise a membrane photobioreactor (PBR), the PBR comprising a liquid medium, at least one photosynthetic microorganism, and at least one outer membrane layer, wherein the membrane layer is comprised of a material that is permeable to gas transfer across the membrane layer; and further comprise a chamber defining a gaseous atmosphere enclosed within, wherein the PBR is located inside the chamber. The devices also comprise a control system which controls the composition of the atmosphere within the chamber. Gas transfer occurs across the membrane layer of the PBR, between the PBR and the atmosphere comprised within the chamber. Systems comprising the devices are provided as well as methods of using the devices for the production of biomass, remediation of wastewater and removal of atmospheric pollutants.

A photobioreactor with a device for emitting electromagnetic radiation, a device for emitting electromagnetic radiation for a photobioreactor, as well as methods of use, are provided. The photobioreactor includes a container for holding fluid media containing biological material and an electromagnetic radiation emitting device. The electromagnetic radiation emitting device has a housing with a source for emitting electromagnetic radiation arranged therein. The electromagnetic radiation emitting device including the housing thereof is arranged inside the container for holding biological material.

This disclosure describes an improved heat transfer system for use in reaction vessels used in chemical and biological processes. In one embodiment, a heat transfer baffle comprising two sub-assemblies adjoined to one another is provided.