The invention relates to a liquid supply interface for a cell culture system for supplying cell cultures found in different cell culture containers with a nutrient medium, wherein the liquid supply interface comprises: a housing defining a flow area; a first connection formation for the liquid-transferring connection of a first fluid line to the housing; a second connection formation formed separately from the first for the liquid-transferring connection of a second fluid line to the housing; a third connection formation formed separately from the first two for the liquid-transferring connection of the housing to a third fluid line; a coupling formation formed separately from the connection formations, which is formed for the producible and detachable liquid-transferring coupling contact according to the operation, with a corresponding counter-coupling formation of a cell culture container.

Combining through self-modulation a flow of active media from a working vessel of a bioprocess together with a flow of reference media and making a time- and/or spatially-resolved referenced optical measurement of the active vs reference media in a confined flow region and time, such that the two liquids are measured in substantially identical conditions.

The invention relates to a liquid supply interface for a cell culture system for supplying cell cultures found in different cell culture containers with a nutrient medium, wherein the liquid supply interface comprises: a housing defining a flow area; a first connection formation for the liquid-transferring connection of a first fluid line to the housing; a second connection formation formed separately from the first for the liquid-transferring connection of a second fluid line to the housing; a third connection formation formed separately from the first two for the liquid-transferring connection of the housing to a third fluid line; a coupling formation formed separately from the connection formations, which is formed for the producible and detachable liquid-transferring coupling contact according to the operation, with a corresponding counter-coupling formation of a cell culture container.

There is disclosed a method for preparation of substrate for use in anaerobic digestion of organic waste. The method can be executed in a substrate pretreatment system, the method comprising: loading of a substrate into the substrate pretreatment system, mixing the substrate with liquid; heating the substrate and liquid to a pre-determined processing temperature; exposing the mixture to radiation; transferring the processed substrate to a bioreactor, during the heating, the method further comprises: degassing the mixture using vacuum extraction; and circulating the mixture past an ultrasonic hydrodynamic radiator.

A fluid supply interface includes at least one supply coupling configuration, at least one discharge coupling configuration, at least one user coupling configuration, and a fluid conduit that connects the supply, discharge, and user coupling configurations to one another. A supply valve is capable of having fluid flow through it or is blocked for flow through it. A discharge valve is capable of having fluid flow through it or is blocked for flow through it. The supply valve is preloaded into a blocking position that prevents flow, and opens by means of a sufficiently large pressure difference between the two sides of the supply valve, against the preload force, for flow through in a direction from the supply coupling configuration toward the fluid conduit. The discharge valve likewise being preloaded into a closed position that prevents flow through it.

Combining through self-modulation a flow of active media from a working vessel of a bioprocess together with a flow of reference media and making a time- and/or spatially-resolved referenced optical measurement of the active vs reference media in a confined flow region and time, such that the two liquids are measured in substantially identical conditions.

An automated method for culturing stem cells using a robotic liquid handling system including a translatable bed and a movable multi-channel pipette. The method includes the steps of: locating a first multi-well cell culture plate and a multi-trough plate on the bed; placing a suspension of stem cells in at least one trough of the multi-trough plate; using the multi-channel pipette, transferring a portion of the suspension of stem cells to each well of the first multi-well cell culture plate such that at least two of the wells of the first multi-well cell culture plate have different densities of stem cells; selecting a well of the first multi-well cell culture plate having a desired density of stem cells; locating a second multi-well cell culture plate on the bed; and using the multi-channel pipette, transferring the cells of the selected well to a plurality of wells of the second multi-well cell culture plate.

An automated method for culturing stem cells using a robotic liquid handling system including a translatable bed and a movable multi-channel pipette. The method includes the steps of: locating a first multi-well cell culture plate and a multi-trough plate on the bed; placing a suspension of stem cells in at least one trough of the multi-trough plate; using the multi-channel pipette, transferring a portion of the suspension of stem cells to each well of the first multi-well cell culture plate such that at least two of the wells of the first multi-well cell culture plate have different densities of stem cells; selecting a well of the first multi-well cell culture plate having a desired density of stem cells; locating a second multi-well cell culture plate on the bed; and using the multi-channel pipette, transferring the cells of the selected well to a plurality of wells of the second multi-well cell culture plate.

An automated method for culturing stem cells using a robotic liquid handling system including a translatable bed and a movable multichannel pipette. The method includes the steps of: locating a first multi-well cell culture plate and a multi-trough plate on the bed; placing a suspension of stem cells in at least one trough of the multi-trough plate; using the multi-channel pipette, transferring a portion of the suspension of stem cells to each well of the first multi-well cell culture plate such that at least two of the wells of the first multi-well cell culture plate have different densities of stem cells; selecting a well of the first multi-well cell culture plate having a desired stem cell density; locating a second multi-well cell culture plate; and using the multi-channel pipette, transferring the cells of the selected well to a plurality of wells of the second multi-well cell culture plate.

The present invention provides a self-draining well plate cassette and an automated system for cultivating C. elegans comprising a housing, a well plate assembly having at least two of the self-draining well plate cassettes, a liquid dispensing assembly operated by a first three axes positioner, a wash and imaging assembly operated by a second three axis positioner, a reagent assembly, a pipette tip holder, and a controller. The present invention further provides a method of using the above-described system to cultivate C. elegans.