A storage assembly for storing a plurality of specimens includes a frame, a plurality of modular storage units for perfusion and/or incubation of one or more specimens removably coupled to the frame, a sample transfer apparatus configured to retrieve a specimen holder from a chosen modular storage unit of the plurality of modular storage units, and a control unit communicatively coupled to the sample transfer apparatus. The control unit is configured to cause the sample transfer apparatus to retrieve a specimen from a modular storage unit of the plurality of modular storage units and deliver the specimen to a delivery position.

A fully automatic cell culture method and system thereof based on mechanical arm are disclosed, and the method includes: acquiring raw blood; performing T cell sorting for the raw blood; performing amplification culture for the sorted T cells; performing CAR transfection for the amplification cultured T cells; performing re-amplification culture for the CAR transfected T cells; treating another batch of T cells during the amplification culture and the re-amplification culture; acquiring the cultured CAR-T cells.

Described are systems, methods, and devices relating to normothermic extracorporeal support of an organ, tissue, or bioengineered graft comprising cross-circulation (XC) perfusion for prolonged periods (days to weeks) via an XC perfusion circuit in connection with an extracorporeal host (e.g., animal, patient, organ transplant recipient) are disclosed. The XC perfusion circuit comprises auto-regulation of blood flow based on the trans-organ blood pressure difference between arterial and venous pressure. Recipient support enabled 36 h of normothermic perfusion that maintained healthy lungs with no significant changes in physiologic parameters and allowed for the recovery of injured lungs. Extended support enabled multiscale therapeutic interventions in all extracorporeal lungs. Lungs exceeded transplantation criteria.

The bioreactor may include a single-use, rigid-sided bioreactor vessel containing a fluid to be mixed and a vertical mixing wheel. A perfusion dip tube with a screen filter incorporated on the end is secured to the vessel from the top lid and partially submerged in the fluid, preferably into close proximity with an outer circumference of the vertical mixing wheel. The screen filter of appropriate mesh size allows spent cell culture medium to be withdrawn from the bioreactor vessel while retaining cell aggregates or microcarriers on which cells are attached and growing in the vessel. Alternating flow of fluid out from and into the dip tube enables removal of spent medium and unclogging of the dip tube filter.

Described are systems, methods, and devices relating to normothermic extracorporeal support of an organ, tissue, or bioengineered graft comprising cross-circulation (XC) perfusion for prolonged periods (days to weeks) via an XC perfusion circuit in connection with an extracorporeal host (e.g., animal, patient, organ transplant recipient) are disclosed. The XC perfusion circuit comprises auto-regulation of blood flow based on the trans-organ blood pressure difference between arterial and venous pressure. Recipient support enabled 36 h of normothermic perfusion that maintained healthy lungs with no significant changes in physiologic parameters and allowed for the recovery of injured lungs. Extended support enabled multiscale therapeutic interventions in all extracorporeal lungs. Lungs exceeded transplantation criteria.

Described are systems, methods, and devices relating to normothermic extracorporeal support of an organ, tissue, or bioengineered graft comprising cross-circulation (XC) perfusion for prolonged periods (days to weeks) via an XC perfusion circuit in connection with an extracorporeal host (e.g., animal, patient, organ transplant recipient) are disclosed. The XC perfusion circuit comprises auto-regulation of blood flow based on the trans-organ blood pressure difference between arterial and venous pressure. Recipient support enabled 36 h of normothermic perfusion that maintained healthy lungs with no significant changes in physiologic parameters and allowed for the recovery of injured lungs. Extended support enabled multiscale therapeutic interventions in all extracorporeal lungs. Lungs exceeded transplantation criteria.

The invention relates to a microplate reader and a respective method, wherein the microplate reader comprises at least one measuring device and a holding device for accommodating at least one microplate and for positioning the samples-containing wells of this(these) microplate(s) in relation to the at least one measuring device. The at least one measuring device is used for detecting light which is emitted by samples in wells of a microplate inserted in this microplate reader and/or which is influenced by samples transilluminated by light in wells of a microplate inserted in this microplate reader. The microplate reader comprises a control unit for controlling the temperature of a gas atmosphere surrounding the wells containing the samples of microplates used in this microplate reader.

A growth chamber system includes a power source and an inner chamber. An access opening is included to the inner chamber. An outer chamber surrounds at least one wall of the inner chamber. A temperature sensor is included which monitors the temperature inside the inner chamber. A heater and pump are fluidly connected to the outer chamber and a working fluid is circulated by the pump through the heater and outer chamber such that an increase in temperature of the working fluid produces a temperature increase within the inner chamber by contact of the working fluid with an outer surface with at least one wall of the inner chamber. A rotor blade is positioned within the inner chamber and connected to an electric motor. At least one controller is included and connected to the fluid heater, pump, and electric motor and configured to control temperature and rotation speed.

The invention discloses a bioreactor apparatus (1;101;201;301) for cultivation of cells comprising: a) a disposable bioreactor vessel (2) with one or more walls (3,4,5) defining an inner volume (6), at least one port (10) in a wall, wherein the disposable bioreactor vessel is positioned in a rigid support structure (8;108); and b) a heater (9;109;209;309), capable of heating an amount of culture medium to a target temperature in the range of 55-95 C., while the amount of culture medium is being confined in or conveyed to the inner volume.

A biogas plant produces both methane and liquid fertilizer by fermenting biomass. The plant includes a fermenter, a percolate tank and a sanitation tank located inside the percolate tank. Dry fermentation takes place in the fermenter and generates methane and a percolate. The percolate is circulated between the fermenter and the percolate tank. Percolate that is returned from the percolate tank to the fermenter is sprinkled over the biomass. A portion of the percolate is transferred from the percolate tank into the sanitation tank. The percolate in the sanitation tank is sanitized by heating to a Celsius temperature between 45 and 65 for a period of at least five days. The percolate in the sanitation tank is heated using both a heating device in the sanitation tank as well as heat generated from a thermophilic fermentation reaction occurring in the percolate tank. The sanitized percolate is used as liquid fertilizer.