Bioreactor systems and controlled operation of bioreactor systems are disclosed herein. The bioreactor systems can include at least one bioreactor chamber, at least one reservoir, a plurality of sensors, and a fluid circuit. The operational methods disclosed herein are directed towards growing cells or tissue while measuring various parameters, and a controlled operation of the various parameters during the operation of the bioreactor systems. The controlled operation of the parameters includes, for example, cell concentration; a rate of flow; a volume; a pH; a temperature; a level of oxygen; a level of carbon dioxide; a level of bicarbonate ion; nutrient compound; and any combination thereof.

A device for in vitro culture of embryos includes: an array having at least one well formed therein; and a bottom surface formed in the well and made of polydimethylsiloxane (PDMS). The use of the culture vessel having the well bottom surface made of PDMS has the effect of further increasing the blastocyst formation rate of embryos compared to the use of conventional arrays made of plastic.

Provided is a cell-containing container including nerve cells and a medium, in which the nerve cells adhere to a culture surface of the cell-containing container, an adhesion area between the nerve cells and the culture surface is 0.5 mm.sup.2 or more per 80,000 nerve cells, and a concentration of glucose in the medium is 1 g/L or higher.

Provided are an evaluator including an analysis unit that evaluates a differentiation level of a cultured cell on the basis of a content of a component in a culture supernatant of the cultured cell, and an automatic cell culture system including the evaluator and an automatic culture device.

A method and system for performing operations on cells on an instrument wherein a network of instruments for performing operations on cells is provided. The operations are controlled by protocols run on the instruments and data relating to the protocols is metadata. A database of protocols and metadata relating to the protocols is maintained and results from the performance of the operations on cells by the instruments in the network is stored in the database. The results for the protocols and metadata are analyzed to vary at least one of the stored protocols and the stored metadata to improve future results.

A bioreactor assembly component can include a length of tubing having an integrated pump head along its length, with a portion of the tubing extending between the pump head and a container, such as a single-use bioreactor. The integrated pump head can be configured to engage with an external driving component to control the operation of the integrated pump head without breaching the sterility of the integrated pump head. The integrated pump head can include only components which can be gamma sterilized, allowing the bioreactor assembly component to be sterilized and stored before use. The bioreactor assembly component can be used with a control module including a pump driver configured to engage with and control the operation of the integrated pump driver, and can include components which can monitor and control the operation of a bioprocess within a single-use bioreactor.

A method and system for control of a microbial fermentation process involving co-fermentation of sugars from lignocellulosic biomass to fermentation products by means of fermentation microorganisms is provided. A residual sugar indicator parameter RSI is measured, which parameter directly or indirectly indicates the concentration of residual sugars, during fermentation in the fermentation vessel (31). The amount of fermentation media added to the fermentation vessel is automatically adapted in a predetermined manner in response to the residual sugar indicator parameter, so as to achieve efficient co-fermentation of sugars to fermentation products.

An information processing apparatus according to the present technology includes an acquisition section and a prediction section. The acquisition section acquires a plurality of chronologically captured observation images of a fertilized egg, and culture environment information regarding the fertilized egg. Using the plurality of observation images and the culture environment information, the prediction section predicts a period of time necessary until the fertilized egg reaches a specified form of development, and a quality of the fertilized egg in the form of development.

A culturing process according to the present embodiment includes: a step of carrying out the setting of a culture apparatus 1 (Step S100); a step of carrying out the conditioning of a pH sensor 40 (Step S200); and an actual culturing step (Step S300). More specifically, in the process, the setting of the culture apparatus 1 (Step S100) is carried out; and, after the completion of the preceding step, the step of carrying out the conditioning of the pH sensor 40 (Step S200) is carried out; and subsequently the actual culturing step is carried out using the culture apparatus 1 (Step S300).

A method and apparatus for screening of cell lines and culture conditions includes a target value setting step that sets a target value relating to a metabolic reaction of a culture cell; a first culturing step; an analysis step; an analysis value calculation step that performs a metabolism analysis based on an analysis result from the analysis step and calculates an analysis value; a culture condition computation step that computes, using a correlation model, a culture condition giving an analysis value most close to the target value, and modifies the culture condition set in the first culturing step to the computed culture condition; a second culturing step that cultures a cell under the modified culture condition; and a screening step.