The gas dissolution device includes a dissolution vessel storing a part of culture solution in a culture vessel, a gas supply pipe connected to a carbon dioxide cylinder and supplying carbon dioxide through an end inserted into the dissolution vessel, a gas discharger provided at the gas supply pipe and turning the carbon dioxide into microbubbles, and a mass flow controller controlling a flowrate of the carbon dioxide flowing in the gas supply pipe. Here, a water depth from the gas discharger to a liquid level of the culture solution in the dissolution vessel is set deeper than a water depth of the culture solution in the culture vessel.

A bioreactor may comprise a head-plate configured to provide coupling features to attach selected components to the bioreactor. The bioreactor may also comprise impeller and sparger, and the sparger is positioned at a pre-determined distance from the impeller based at least in part on a spatial configuration of the head-plate. The bioreactor further comprises a vessel defining a hollow enclosure for receiving a culture medium, and an opening of the vessel is covered by the head-plate.

The gas collection device includes a constant temperature chamber whose interior is maintained at a set temperature; a culture flask unit located inside the constant temperature chamber and culturing bacteria therein; a mass flow controller located outside the constant temperature chamber and connected to the culture flask unit through an injection flow path to control a gas injected into the culture flask unit through the injection flow path; and an impinger located outside the constant temperature chamber and connected to the culture flask unit through a discharge flow path to receive a gas discharged from the culture flask unit through the discharge flow path in real time.

The device for a cell suspension culture comprises a culture chamber comprising first and second axisymmetric-shaped walls having at least a partially curved radial section the concavity thereof facing the inside of the culture chamber, the walls delimiting an inner compartment and form an inner continuous surface; an inlet opening provided in the first wall and adapted to enable a fluid to enter; a supplying conduit connected to the inlet opening; and a valve member disposed inside the culture chamber adapted to tightly close or open the inlet opening. The device comprises also a device for moving the valve member, disposed outside the culture chamber.

The present invention provides a parallel bioreactor system, comprising: an oscillator for generating oscillating motion; a plurality of culture vessels mounted on the oscillator, wherein each culture vessel is provided with an inner cavity, the inner cavity comprises a cylindrical portion at the upper part and an inverted truncated conical bottom at the lower part, a cross section of the cylindrical portion is consistent with the cross section of the top of the inverted truncated conical bottom, and the bottom of the cylindrical portion is joined with the top of the inverted truncated conical bottom; disposable culture bags arranged in the inner cavities of the culture vessels and used for accommodating culture solution, wherein each disposable culture bag is provided with a multifunctional cover plate, and the multifunctional cover plate is connected to the top of the culture bag to seal the culture bag, and is provided with a plurality of connection holes leading to interior of the disposable culture bag; and a control system, wherein the control system controls the oscillating motion of the oscillator and parameters of the culture solution in the disposable culture bags.

Methods and apparatus of bioreactors for therapeutic cells manufacturing are provided herein. In some embodiments, a bioreactor includes: an upper bioreactor reservoir configured to perform multiple cell therapy manufacturing process steps including genetic modification and expansion to a plurality of cells disposed therein, wherein the upper bioreactor reservoir includes a plurality of ports for delivering fluids into and out of the upper bioreactor reservoir; a lower bioreactor compartment configured to hold a suspension comprising a molecular species; and a membrane disposed between the lower bioreactor compartment and the upper bioreactor reservoir, wherein the membrane includes a plurality of micro-straws extending through the membrane and into the upper bioreactor reservoir to transfect the plurality of cells with the molecular species.

System and method for providing biocompatible, nutrient filled media to the Human Cells, Tissues, and cellular and tissue-based Products (HCT/P) while removing wastes. The present teachings provide for sensing the characteristics of the media, and modifying the characteristics when necessary. The present teachings can also provide components that can provide fluid pumping integrated with fluid gas exchange, and sensing of fluid characteristics at consistent times during the fluid flow cycle. System and method control multiple bioreactors from a centralized media reservoir, while fluidically isolating the bioreactors from cross-contamination.

The present disclosure provides a three-dimensional cell culture method and a cell culture apparatus using the same. The cell culture method includes lowering, when culturing cells on at least one surface of a porous support, a concentration of a cell-secreted substance in a lower region of the other surface of the porous support. The cell culture apparatus includes: an upper chamber including an opening, porous support having at least one surface on which cells are cultured, and a cell culture space in which a culture medium is housed; a lower chamber including a space in which at least one upper chamber is disposed; and a device lowering a concentration of a cell-secreted substance in a lower region of the other surface of the porous support.

An electrochemical measurement device for measuring a chemical substance generated or consumed in a biological sample in a solution includes electrode surfaces, a spacer, and at least one wall plate. The electrode surfaces, the spacer, and the wall plate are arranged on the same flat surface. Each of the electrode surfaces has a diameter d.sub.el not more than 80 μm. A height of the spacer has a predetermined value within a range given by h=21.8(d.sub.el+0.8)/(d.sub.el+9.7)±5 [μm]. The spacer has a structure in which an enclosed three-dimensional region is not formed by the biological sample, the flat surface, and the spacer while the biological sample is in contact with the spacer. The wall plate has a property of being impervious to a dissolved substance in the solution and has a height not less than the height of the spacer. Two of the electrode surfaces are separated by the wall plate.

Methods for determining ethylene concentration in an ethylene oligomerization reactor using an ultrasonic flow meter are described, and these methods are integrated into ethylene oligomerization processes and related oligomerization reactor systems.