The present disclosure provides an automated method of producing viral vectors, utilizing engineered viral vector-producing cell lines, or packaging cells, within a fully-enclosed cell engineering system. Exemplary viral vectors that can be produced include lentivirus vectors, adeno-associated virus vectors, baculovirus vectors and retrovirus vectors.

Embodiments of the provided technology relate to a gas flow and liquid flow regulation system within a cell culture instrument. Embodiments of the gas and liquid flow regulation system include a pressurizable gas-mixing chamber, a cell culture compartment that includes a cell culture vessel having and a gas space, and a gas flow system. The gas flow system is driven by gas pressure, and is adaptable to provide an atmospheric condition of hypoxia and hyperbaric pressure within the cell culture compartment. The liquid flow regulation system is driven by hydraulic pressure.

Herein is reported a bioreactor comprising a cultivation vessel and a reactor head plate, wherein the cultivation vessel has a working volume of from 20 ml to 350 ml and comprises two or more in-situ sensors, wherein the reactor head plate comprises an in-situ sensor port, wherein to the in-situ sensor port at least one in-situ glucose sensor and one in-situ pH sensor are connected.

A treatment apparatus for treating biological cell cultures, located in respective storage chambers of a carrier, includes a work deck with one or more bearing spots for bearing the carrier. Each of the bearing spots includes a bearing region corresponding to an area of the carrier. The treatment apparatus additionally includes nozzles for producing a gas flow in a space above the work deck. To avoid liquid droplets or cell material being transported from one storage chamber to another as a result of a horizontal gas flow above the carrier during the treatment, the nozzles are arranged outside of and along an edge of the bearing region or within the bearing region and form a gas curtain around the carrier or the respective storage chambers.

The present subject matter discloses a bioreactor apparatus. The bioreactor apparatus comprises a bioreactor vessel configured to culture cells. The bioreactor apparatus furthermore comprises a sensor configured to measure Dissolved Oxygen (DO) in the bioreactor vessel. The DO measurements comprise a plurality of DO values recorded at, at least, a plurality of time instances during operation of the bioreactor apparatus. The bioreactor apparatus furthermore comprises a controller configured to obtain the DO measurements. The controller furthermore is to determine, in real-time or approximately real-time, an oxygen mass transfer co-efficient (kLa) associated with the operation of the bioreactor apparatus. Furthermore, the controller is configured to control, in real-time or approximately real-time, at least one cell culture parameter associated with the operation of the bioreactor apparatus based on the kLa.

A cell culture apparatus is provided. A cell culture apparatus according to one exemplary embodiment of the present invention comprises: a cell culture part comprising an accommodation space in which a plurality of supports for cell culture are disposed; a medium supply part for storing a predetermined amount of medium to be supplied to the cell culture part, and maintaining a predetermined carbon dioxide concentration of the medium stored therein by using carbon dioxide introduced from the outside through a gas supply port; and a pump, which interconnects the cell culture part and the medium supply part so as to circulate, in the cell culture part, a medium stored in the medium supply part.

A movable cell incubator contains: a body, a first lid, a second lid and an electric control unit. The body includes a first internal space, a refrigeration room, and an airtight culture room. The first lid airtightly covers the culture room, the second lid airtightly covers the refrigeration room, and the control unit includes a microprocessor, a power module, a digital/analog conversion module defined between a microprocessor and the power module, a heating module controlling temperature of the culture room, a cooling module supplying cold source to the refrigeration room, a peristaltic pump module, a flow sensing module, a CO2 detective supply module supplying CO2 to the culture room, and a setting display module exposing and fixed on the first lid, with the peristaltic pump module aseptically connected between cell culture media and cell culture bag by multiple conveying tubes.

The present invention relates to a method for producing compressed collagen comprising the steps of a) pressing a gelled collagen in a first pressing direction; and b) pressing the gelled collagen of step a) in a second pressing direction substantially orthogonal to the first pressing direction, wherein the pressing in step a) is carried out at a pressure in the range of 0.01 to 0.05 bar and in step b) at a pressure in the range of 0.5 bar to 2 bar.

The present disclosure provides methods for controlling oxygen concentration during aerobic biosynthesis, e.g., fermentation. The method may comprise feeding an oxygen-containing gas into a vessel including a fermentation feedstock and reacting the fermentation feedstock with the oxygen-containing gas to form a broth including a gaseous phase dispersed within the broth. The gaseous phase may comprise any unreacted oxygen from the oxygen-containing gas. The method further includes reducing the concentration of the unreacted oxygen in the dispersed gaseous phase to less than the limiting oxygen concentration (“LOC”) for flammability before separating the gaseous phase from the fermentation broth. The concentration of the unreacted oxygen in the gaseous phase is reduced by employing oxygen removal schemes or oxygen dilution schemes.

The method for analysis and cell culture comprises the following steps: generating a train (14) of ordered drops (16) in a carrier fluid (40), the train (14) of drops (16) comprising at least one culture drop (42), the culture drop (42) comprising a culture medium (50) and at least one cell (4), circulating the train (14) of drops (16) in a tube (10), incubating the train (14) of drops (16) in the tube (10), measuring at least one parameter indicative of the content of the culture drop (42) in the tube (10) at different times, recovering the culture drop (42) at one end (36) of the tube (10),
the steps being carried out in a controlled atmosphere (6).