Provided are an imaging system and an imaging device capable of generating a super-resolution interference fringe image of an object to be observed flowing through a flow channel. A light source that irradiates light in a first direction and irradiates light toward a flow channel through which an object to be observed flows in a second direction orthogonal to the first direction, an imaging sensor that has an imaging surface orthogonal to the first direction and on which a plurality of pixels are two-dimensionally arranged in a manner non-parallel to the second direction and that images light passing through the flow channel to output an interference fringe image, and an information processing device that generates a super-resolution interference fringe image based on a plurality of interference fringe images output from the imaging sensor are included.

A system for evaluating stem cell differentiation includes a storage configured to store a machine learned model that has learned success or failure of cell differentiation for a combination of a stem cell and a differentiation induction method, an acquisition unit configured to acquire a target cell image that is an image of a target cell that is a stem cell to be induced to differentiate and differentiation induction information that is information related to a differentiation induction method applied to the target cell, and a processor configured to output differentiation success or failure information indicating an inference result related to success or failure of differentiation of the target cell into a desired cell type on the basis of the target cell image, the differentiation induction information, and the machine learned model. The differentiation induction information includes information indicating the type of a stimulus given to the target cell.

An apparatus that configures a culture plate for precisely assigning coordinates to a selected colony by assigning fiducials to the culture plate using simple mechanical techniques. The fiducials correspond to the plate center and the center of a barcode label applied to the side of the culture plate by the apparatus. The apparatus then deploys mechanisms to apply coordinates to colonies identified by the user relative to the fiducials. One such mechanism is a webcam pointed at the culture plate that allows a technician to mark the colonies on a display using a computer mouse or equivalent cursor. Another mechanism deploys a laser pointer directed at the colony wherein the apparatus assigns coordinates to the location of the colony onto which the laser pointer is directed. In a third mechanism, the user views the surface of the culture plate through a viewfinder and manually registers coordinates when the view finder cross hairs are over a target colony. The selected colonies are assigned precise coordinates with reference to the two fiducials. Software is provided such that pixels in the image of the culture plate correspond to the coordinates of the culture dish relative to the fiducials.

An image analyzer including: an area setting unit configured to extract a scratch area that is an area having no cells from a reference image selected from a plurality of images acquired by imaging cells in a time series and set a reference region corresponding to the scratch area in the plurality of images; a calculation unit configured to calculate an area of a cell region within the reference region and/or the ratio of an area of the cell region to the reference region from the plurality of images; and a control unit configured to cause a display device to display a change in a time series of the calculated area of the cell region and/or the ratio of the area of the cell region to the reference region.

An interference image acquisition apparatus includes a light source, a beam splitter, a second reflection mirror, an imager, and a first reflection mirror. A cell is placed on one side of a transparent material, and the first reflection mirror is placed on the other side of the transparent material. In a two-beam interferometer, an optical path difference between an optical path length of a first light beam reflected by the first reflection mirror and an optical path length of a second light beam reflected by the second reflection mirror is set to a coherence length of light output from the light source or less. The imager acquires an interference image in a state in which the cell is placed at a position conjugate to an imaging plane in a first optical system between the imaging plane and the first reflection mirror.

A storage assembly for storing a plurality of specimens includes a frame, a plurality of modular storage units for perfusion and/or incubation of one or more specimens removably coupled to the frame, a sample transfer apparatus configured to retrieve a specimen holder from a chosen modular storage unit of the plurality of modular storage units, and a control unit communicatively coupled to the sample transfer apparatus. The control unit is configured to cause the sample transfer apparatus to retrieve a specimen from a modular storage unit of the plurality of modular storage units and deliver the specimen to a delivery position.

Cartridges for manufacturing a population of cells suitable for formulation as a cellular therapeutic are disclosed herein, along with systems and instruments for operating the cartridges and performing methods to generate the population of cells suitable for formulation as a cellular therapeutic. The population of cells suitable for formulation as a cellular therapeutic can be immunological cells, such as T lymphocytes, including endogenous T cells (ETCs), tumor infiltrating lymphocytes (TILs), CAR T-cells, TCR engineered T-cells, or otherwise engineered T-cells. The systems and methods can be largely automated.

A platform for testing cell response to biochemical agents. The TrophoWell™ includes a well which contains a gel, and a plurality of capillaries that open into it. Cells and various biochemical agents such as drugs and growth factors are flown through those capillaries. The platform allows for the evaluation of cell response by imaging. The platform is a cost effective testing platform and can be used in the fields for drug discovery and personalized medicine.

Disclosed herein are platforms, systems, and methods including a cell culture system that includes a cell culture container comprising a cell culture, the cell culture receiving input cells, a cell imaging subsystem configured to acquire images of the cell culture, a computing subsystem configured to perform a cell culture process on the cell culture according to the images acquired by the cell imaging subsystem, and a cell editing subsystem configured to edit the cell culture to produce output cell products according to the cell culture process.

A system for dissociating cells from a cell culture vessel. The system comprises an imaging system configured to image a plurality of cells in a cell culture vessel being dissociated from at least one surface of the cell culture vessel by at least one cell dissociation agent; and at least one controller coupled to the imaging system and configured to: control the imaging system to capture a sequence of images of at least some cells in the plurality of cells during dissociation; and identify when to neutralize the at least one cell dissociation agent using the sequence of images.