Production of “regenerative medicine products” is facilitated using a quality by design (QbD) approach. A production device produces a medical product and analyzes a starting material and a central management device determines processing conditions in the production device. By transmitting and receiving data pertaining to the starting material between the production device and central management device data, the medical product is produced while production conditions therefor are continuously optimized. Thus, it is easy to produce a medical product while reducing or eliminating effects from changes in cells and tissues over time, from oscillation during transport, and from changes in surrounding environment such as changes in temperature, and to produce the desired medical product even when there are individual differences in the starting material.

Disclosed is a cell image analysis device 2 including: a display unit 52; a storage unit 31 which stores learning data which is a set of data of a cell image and data of an analyzed image in which a region of interest included in the cell image is specified, and a discriminator which is created using a learning model constructed by machine learning using the learning data; a test data input receiver 36 configured to receive an input of test data; a discriminator evaluation unit 37 configured to evaluate the discriminator using the test data; an evaluation result storing unit 38 configured to store evaluation data including an evaluation result of the discriminator and the test data used for the evaluation in association with the discriminator; and a display processor 39 configured to display the test data and/or the evaluation result associated with the discriminator on a screen of the display unit in response to an input for selecting the discriminator.

A device for evaluating progression of differentiation from pluripotent stem cells to pigment-containing cells includes a first irradiation unit configured to irradiate cells in a vessel with light in a melanin absorption wavelength band, a first detection unit configured to detect photo-acoustic waves generated from the cells irradiated with the light, and a processor configured to output an evaluation result relevant to the progression of the differentiation of the cells to the pigment-containing cells, based on intensity of the detected photo-acoustic waves detected by the first detection unit.

The technology disclosed herein relates to, at least in part, a removable imaging cassette. An illumination plate is configured to transmit light. A cassette body defines an outer surface and a cassette cavity within the outer surface, where the outer surface surrounds the illumination plate. The cassette cavity abuts the illumination plate. A light source is disposed in the cassette cavity. Mating electrical contacts extend through the outer surface of the cassette body, where the mating electrical contacts are operably coupled to the light source.

Aspects of the present disclosure relate to a method of colony enumeration. The method includes identifying colony forming units of microorganisms in a combined image using a pretrained deep learning model on a colony enumeration device. The method can include providing a plurality of identification characteristics of the colony forming units to an interaction component such that the interaction component can project at least some of the plurality of identification characteristics onto the combined image.

One or more embodiments of the present disclosure relate to a culture device, which comprises: an accommodation base, an accommodation chamber disposed on the accommodation base. The accommodation chamber includes a culture chamber for accommodating one or more cultures. The culture chamber includes a chamber bottom wall and a chamber side wall surrounding the chamber bottom wall. A culture solution channel is formed between an outer side of the chamber side wall and an inner wall of the accommodation chamber. At least part of the chamber side wall includes a membrane material, and a plane where the membrane material is located intersect with the chamber bottom wall.

Apparatus, systems and methods for the adaptive passage of a culture of cells and apparatus and methods for dissociating cell colonies are described. The systems may include an imaging module, a pipette module, a handling module, and/or a stage module. Coordinated operation of the modules, optionally in an automated manner, is effected by at least one processor based on one or more characteristics of the culture of cells calculated from one or more images captured at more than one time point. A first apparatus for adaptive passage of a culture cells includes an imaging module and at least one processor, which apparatus may be included in the systems or used in the methods. A second apparatus for dissociating cell colonies, may also be included in the systems or used in the methods, includes impact bumper(s) collidable with impact bracket(s) to transmit a dissociative force to a culture of cells.

A method for pre-scanning a transparent cell culture plate with a plurality of wells to improve focus using a plurality of z-axis images and a method for focusing an optical system on a transparent cell culture plate by performing a Fourier transform on image data at different focus distance steps to reveal a pattern and using the pattern at each step to determine the focus distance.

Provided is a biological specimen holder for positioning multiple specimens for imaging by a light-sheet microscope. The specimen holder allows developing plant embryos, small intact animals, or organs to be imaged in the light-sheet microscope in a single setting. The specimen holders significantly improve the imaging conditions with respect to the standard glass capillary system. Also provided is a semi-automatic image processing pipeline that quantifies cell divisions of plants imaged with both the glass capillary and the novel chambers. Plants imaged using the specimen holder undergo cell divisions for a period at least 16 times longer than those imaged with a glass capillary system and allow increased sample throughput and the option of incorporating light emitting diode (LED) lights to generate a light-controlled environment are also advantages.

A culturing device includes a microplate including a plurality of vessels, each of the vessels having a bottom surface having light transmittance and an opening at an upper portion, a lid having light transmittance, and an intermediate plate having light transmittance sandwiched between the lid and the microplate. The intermediate plate has a plurality of convex portions on a surface thereof facing the microplate and provided with a plurality of through holes corresponding to the convex portions that are disposed so that when the intermediate plate and the microplate are overlapped, each of the plurality of convex portions is inserted into each of the plurality of vessels and each of the plurality of through holes coincides with the opening of each of the plurality of vessels. The lid comes into contact with the intermediate plate so as to close the plurality of through holes provided in the intermediate plate.