Metal bioremediation and metal mining strategies can include compositions and methods.

The present invention relates to a method for obtaining yeast proteins comprising the following steps: a) providing a yeast cream; b) exposing this yeast cream to a thermal plasmolysis at a temperature between 70 and 95? C. for a time of between 30 seconds and 4 hours; c) subjecting the whole to the activity of at least one ribonuclease and a glucanase, sequentially or simultaneously, at a temperature between 40 and 65? C. for a period of between 8 and 24 hours; and d) separating the insoluble fraction from the soluble fraction; wherein the insoluble fraction collected in step d) is taste-free, has a nucleotide content less than 3% and a true protein content of at least 72%.

The present invention relates to a yeast extract having a high tripeptide content and a high dry matter content using a yeast cell containing tripeptide and a method for preparing the same.

Disclosed herein is a method for separating a protein from a microorganism comprising a cell wall. Further compositions comprising a first fraction, a second fraction or both, derived from a microorganism comprising a cell wall and comprising a protein content between 10% and 90% by weight of the fraction, are also disclosed.

A personal care composition includes an ozone-stressed yeast lysate; and a preservative, in which the preservative is selected from phenoxyethanol, isopropyl alcohol, benzyl alcohol, propylene glycol, butylene glycol, pentylene glycol, methylparaben, propylparaben, butylparaben, benzalkonium chloride, Quaternium 15, methylisothiazolinone, methylchloroisothiazolinone, DMDM hydantoin, imidazolidinyl urea, diazolidinyl urea, butylated hydroxytoluene, tocopherol, triclosan, chlorohexidine digluconate, and combinations thereof, and the ozone-stressed yeast lysate is produced by a method including exposing growing yeast to ozone by aerating the yeast for a time period of between five minutes and seventy-two hours with an aeration gas having an ozone concentration of from 0.0001 millimolar (mM) to 1.0 millimolar (mM) based on the total volume of the fermentation broth to produce ozone-stressed yeast lysing the ozone-stressed yeast to produce an ozone-stressed yeast lysate comprising water-soluble and water-insoluble components; and separating the water-soluble components from the water-insoluble components to produce an ozone-stressed yeast lysate containing the water-soluble components.

Provided herein are compounds, compositions, and methods for recovery of one or more water-immiscible compounds from microbial biomass.

The present invention belongs to the technical field of condiment preparation, and specifically relates to an astaxanthin-containing condiment and a preparation method therefor. In this method, first a low-speed bead milling method is used to break the cell wall of the yeast, such that astaxanthin is directly emulsified in water, and the concentration of astaxanthin can reach 1043.17 mg/L, avoiding the use of emulsifiers and organic solvents; then a small amount of Angel yeast compound enzyme is added for enzymolysis, with the yield of amino nitrogen as high as 3.51% to 3.65% and the yield of solid matter as high as 47.18% to 49.22%; and finally the gelatinized porous starch solution and gelatin are sequentially added, with the encapsulation rate and drug-loading rate of the obtained astaxanthin-containing microcapsule powder being 75.62% to 88.5% and 1.55-10.42 mg/g, respectively. The microcapsule powder has bright color, and high astaxanthin stability and water solubility. The preparation method has the advantages of short time and mild conditions, which can reduce the industrial application cost of Phaffia rhodozyma.

Described herein are biological devices and extracts useful for detecting Alzheimer's disease and/or concussions. The biological devices include microbial cells transformed with a DNA construct containing genes for producing -amyloid precursor protein, microtubule associated protein tau, adipose triglyceride lipase, acyl-CoA dehydrogenase, and O-linked N-acetylglucosamine transferase. In some instances, the biological devices also include a gene for enhanced green fluorescent protein. Methods for using the devices to diagnose or detect Alzheimer's disease and/or concussions are also provided herein.

Methods and systems used to extract lipids suitable in production of biofuels from a fermentation broth may include using heat to pre-treat the fermentation broth in order to more easily extract a product from oleaginous microorganisms in the broth. Additionally or alternatively, a combination of enzymes including amylase, 1-4 mannosidase, and 1-3 mannosidase may be used to break down cell walls of the oleaginous microorganisms. Residual broth water may be recycled and used as imbibition water for washing a process feedstock to extract sugar.

The invention relates to a method of cell lysis for forming at least one pore in the cell wall and/or cell membrane of a cell in an aqueous medium for release of at least one intracellular target protein and/or lipid from the cell into the aqueous medium, including subjecting the cell with the intracellular target protein and/or lipid in the aqueous medium to an electrohydraulic comminution; and separating the target protein and/or lipid from the cell.

The electrohydraulic comminution is brought about by a pulse current source. The cell in the aqueous medium is subjected to electrical impulse discharges between at least two electrodes of an underwater radio path of a container at a specific operating voltage, impulse energy, and pulse frequency, and at least one pore is formed in the cell wall and/or cell membrane.