A device for analyzing a biological sample which includes a separation and detection chamber into which an injection channel and a discharge channel open, a filter separating the chamber into two distinct spaces so as to define a first space into which the injection channel opens and a second space into which the discharge channel opens, the filter having a porosity suitable for the separation to be carried out, a rough bearing surface having a surface roughness parameter suitable for carrying out a mechanical lysis of the biological species present in the sample, the bearing surface being arranged in the first space, a flexible membrane arranged opposite the rough bearing surface relative to the filter and blocking an opening made through the housing.

The present invention relates to containers for holding liquid samples. The containers may be useful for mixing a liquid sample or lysing cells in a liquid sample. The invention also relates to methods of using the containers of the invention.

The present invention provides a method for detecting a microorganism in a sample, the method comprising: a) filtering a sample through a filter to entrap any microorganisms present in the sample; b) treating the filter to release genomic material or DNA from the entrapped microorganisms; c) amplifying the genomic material or DNA released from the entrapped microorganisms; and d) identifying specific regions of the genomic material or DNA to determine the presence, identify the species or quantify the approximate number of any entrapped microorganisms.

In an example implementation, a method of cell lysis includes moving cell fluid from a first reservoir through a microfluidic channel toward a second reservoir, activating a lysing element multiple times as a cell from the cell fluid passes through the microfluidic channel, and moving lysate fluid that results from the activating through the microfluidic channel and into the second reservoir.

A method for algae disruption includes: a thermal treatment of microalgae belonging to Heterokontophyta at a pH of 3.5 or more and 9.5 or less and a temperature of 40 C. or more and 65 C. or less; and a physical treatment of the microalgae using a high pressure dispersion apparatus, the physical treatment following the thermal treatment.

Apparatus and method for lysing and recovering released material, such as proteins or other biomolecules. Sample material including cells may be at least partially frozen, thawed, cells lysed and biomolecules recovered in a single conduit, e.g., as the sample flows through the conduit. The conduit may include different zones for sample treatment, including a freezing zone, thawing zone, lysing/disassociation zone, etc., and protein or other recovered material may be delivered directly from the conduit to analysis equipment, such as a mass spectrometer. This allows a flow-through type processing of cells that avoids handling and transfer of sample material between different sample holders.

The present invention relates to a method for the deformation and/or fragmentation of a cell, spore or virus, the method comprising: (i) bringing a liquid sample containing the cell, spore or virus into contact with a first surface of a vibratable plate having at least one aperture, and causing the plate to vibrate; and (ii) passing the sample of the cell, spore or virus through the at least one aperture in the vibrating plate so as to cause deformation and/or fragmentation of the cell, spore or virus. It also concerns a device for carrying out the method.

In vitro and in vivo application of sub-atmospheric, negative pressure on growth factor starting material, such as whole blood, extracts growth factors from the platelet granules of the growth factor starting material in a non-destructive medium without activating the clotting process. The extracted growth factors are released into a growth factor composition containing blood plasma, extracellular fluid or interstitial fluid depending upon the type and location of the growth factor starting material. The growth factors have a weight of about 70-76 kDaltons and are applied in either a filtered or unfiltered state topically to the area of a surface wound to effect healing. The extracted growth factors are also injected into soft tissue, such as a torn tendon, to promote tissue growth and healing. The growth factors are released in one method from a patient's own blood. In another method the growth factors are released from a whole blood source and freeze dried by lyophilization. Then at a later date, the freeze-dried product is reconstituted by normal saline for treatment of a patient's wound, for use in a surgical procedure, or for tissue regeneration.

The present invention relates to a reagent for the disruption of cell material, containing an internal standard that is completely integrated into the reagent for control and evaluation of the completeness of disruption of the cell material and subsequent steps, comprising a step selected from sample preparation, extraction, enrichment, isolation, purification, reverse transcription, amplification and detection of the cell components obtained from the disrupted cells, or a combination of a plurality or all of these steps.

A method for producing membrane polysaccharides from an organism selected from micro-organisms, unicellular organisms and filamentous fungi, the method including at least one step of extracting the membrane polysaccharides as well as a smaller-scale extraction of the soluble proteins, by mechanical treatment of the organism in a ball mill or by physical treatment of the organism by means of ultrasounds.