The present disclosure provides non-parenteral compositions comprising a recombinant Spirulina comprising at least one exogenous therapeutic. Compositions of the present disclosure can be used as vaccines and/or therapeutic drugs. The present disclosure also provides methods of making recombinant Spirulina comprising at least one exogenous therapeutic, and methods of treatment.

An assembly, methods, and a network for farming and culturing green microalgae (Haematococcus pluvialis) are disclosed. The assembly includes a mechanical support framework; a plurality of cultivation pouches, mechanically coupled to the mechanical support framework, where green microalgae (H. pluvialis) are cultured and harvested; a plurality of light emitting diodes (LEDs), mechanically coupled and supported by the mechanical support framework and arranged in between two cultivation pouches, operable to provide a predetermined light intensities to the cultivation pouches; and an air and gas supply and distribution unit, mechanically coupled and supported by the mechanical support framework, operable to supply predetermined amounts of air and carbon dioxide (CO.sub.2) to the cultivation pouches.

The present invention relates to an engineered unicellular Stramenopile microalga comprising a loss of function of the homologous Seipin gene, an in vitro method of producing triacylglycerols (TAG), and uses thereof.

The present disclosure relates to a Schizochytrium sp. Strain, and a method for producing a biomass by using the strain.

A method for the fractionation of fatty acids with a difference of two carbons by molecular distillation with double pass fractionation between eicosapentaenoic and docosahexaenoic acids, and compositions of microorganism oils enriched in eicosapentaenoic or docosahexaenoic acid. The method wherein (i) a reaction step between an microorganism oil including omega-3 polyunsaturated fatty acids in triglyceride form and an alcohol in the presence of a chemical or enzymatic catalyst, (ii) a first step of molecular distillation under high vacuum of the oil from step (i), in a scraper film evaporator coupled to a rectification column including at least seven theoretical plates, and recovery of a first residue and distillate, (iii) a second step of molecular distillation under high vacuum of the step (ii) residue, in the scraper film evaporator, and recovery of a second residue and distillate.

The present disclosure provides a Schizochytrium sp. Strain and its use thereof.

The present invention can: efficiently and readily produce glucose from microalgae that accumulate starch in their cells; and obtain ethanol. During a preparation step of the glucose production method, microalgae are prepared on which a saccharifying enzyme acts on starch accumulated inside the microalgae cells, without disrupting the cell walls. In a saccharification step, starch inside the cells is saccharified and glucose is generated, by adding a saccharifying enzyme to the microalgae without a disruption treatment. The ethanol production method has a step in which, after the saccharification step, the glucose undergoes alcoholic fermentation and ethanol is generated.

The present invention relates to a method for producing a microalgae concentrate with low viscosity, the method comprising a step of adjusting the pH of a microalgae fermentation broth and a heat treatment step, in which utility costs and investment costs can be reduced in a dehydration and drying step to secure price competitiveness for production and commercialization of dried microalgae products.

The present invention relates to a heterotrophic methods of culturing algae, particularly Galdieria species, to produce the valuable pigment phycocyanin. The methods rely on high oxygen saturation and controlled base dosing to provide improved phycocyanin production. The present invention further relates to algal biomass, compositions comprising said biomass or phycocyanin, uses thereof in various products, and a reactor for culturing the algae.

A method for producing a microbial oil includes genetically modifying a labyrinthulid which belongs to the genus Parietichytrium or the genus Schizochytrium by disrupting and/or silencing a 4 desaturase gene, a C20 elongase gene, and/or a C18 elongase gene, or by being transformed with an 3 desaturase gene; culturing the labyrinthulid; and collecting the microbial oil from the labyrinthulid. The labyrinthulid has an activity of synthesizing DHA via a PUFA-PKS pathway in an amount of not more than 1/100 of a total amount of DHA synthesized in the labyrinthulid or no activity of producing PUFAs via the PUFA-PKS pathway, and the labyrinthulid has an activity of producing PUFAs via an endogenous elongase-desaturase pathway.