The present invention is related to a method for increasing the trans-specificity of a beta-carotene oxidase (BCO), particularly insect BCO, to be used in the production of vitamin A aldehyde (retinal) from conversion of beta-carotene, with at least about 75 to 100% of retinal in the trans-isoform.

In preferred embodiments, the subject invention provides two-vessel fermentation systems for producing microbe-based products comprising fungal mycelia and/or spores, and/or bacterial endospores, wherein the systems comprise both a submerged fermentation vessel and a solid state fermentation (SSF) vessel. Advantageously use of the two phases improves the efficiency of producing microorganisms by catering to the different requirements for biomass and/or vegetative cell accumulation as well as the requirements for mycelial growth and/or sporulation.

A structure adapted to traverse a fluid environment exerting an ambient fluid pressure is provided. The structure includes an elongate body extending from a root to a wingtip and encapsulating at least one interior volume containing an interior fluid exerting an interior fluid pressure that is different from the ambient fluid pressure. A method of retrofitting a structure adapted to traverse a fluid environment exerting an ambient fluid pressure, the structure comprising an elongate body extending from a root to a wingtip and having at least one interior volume is also provided. The method includes sealing the elongate body to encapsulate the at least one interior volume containing an interior fluid; associating at least one valve with the at least one interior volume; and modifying interior fluid content via the at least one valve to produce an interior fluid pressure that is different from the ambient fluid pressure.

A structure adapted to traverse a fluid environment exerting an ambient fluid pressure is provided. The structure includes an elongate body extending from a root to a wingtip and encapsulating at least one interior volume containing an interior fluid exerting an interior fluid pressure that is different from the ambient fluid pressure. A method of retrofitting a structure adapted to traverse a fluid environment exerting an ambient fluid pressure, the structure comprising an elongate body extending from a root to a wingtip and having at least one interior volume is also provided. The method includes sealing the elongate body to encapsulate the at least one interior volume containing an interior fluid; associating at least one valve with the at least one interior volume; and modifying interior fluid content via the at least one valve to produce an interior fluid pressure that is different from the ambient fluid pressure.

The present disclosure provides methods for utilizing genetically modified microbes to co-produce 3-hydroxypropionic acid (3-HP) and acetyl-CoA, and derivatives thereof from malonate semialdehyde as a common single intermediate. The disclosure further provides modified microbe that co-produce the 3-HP and acetyl-CoA derivatives from malonate semialdehyde.

The present disclosure provides methods for utilizing genetically modified microbes to co-produce 3-hydroxypropionic acid (3-HP) and acetyl-CoA, and derivatives thereof from malonate semialdehyde as a common single intermediate. The disclosure further provides modified microbe that co-produce the 3-HP and acetyl-CoA derivatives from malonate semialdehyde.

The invention provides a highly active S-cyanohydrin lyase obtained by mutating an amino acid residue at position 103 of a wild-type cassava S-cyanohydrin lyase. The mutation can significantly increase an expression of a mutant enzyme in E. coli and does not require a decrease in temperature when induced. Further mutations at position 128 and other sites were performed to obtain mutants with increased catalytic activity.

The invention provides a highly active S-cyanohydrin lyase obtained by mutating an amino acid residue at position 103 of a wild-type cassava S-cyanohydrin lyase. The mutation can significantly increase an expression of a mutant enzyme in E. coli and does not require a decrease in temperature when induced. Further mutations at position 128 and other sites were performed to obtain mutants with increased catalytic activity.

The invention discloses a bioreactor with a vessel defining an inner volume, agitation means and at least one addition tube, wherein a delivery orifice in the addition tube is located within the inner volume and a check valve is arranged in proximity of the delivery orifice for allowing flow of a fluid in the direction from the addition tube into the inner volume of the vessel and blocking flow in the reverse direction.

The invention discloses a bioreactor with a vessel defining an inner volume, agitation means and at least one addition tube, wherein a delivery orifice in the addition tube is located within the inner volume and a check valve is arranged in proximity of the delivery orifice for allowing flow of a fluid in the direction from the addition tube into the inner volume of the vessel and blocking flow in the reverse direction.