The present disclosure is in the technical field of synthetic biology and metabolic engineering. The disclosure provides engineered viable bacteria. In particular, the disclosure provides viable bacteria with reduced cell wall biosynthesis additionally modified for production of glycosylated product. The disclosure further provides methods of generating viable bacteria and uses thereof. Furthermore, the disclosure in the technical field of fermentation of metabolically engineered microorganisms producing glycosylated product.

Described are a recombinant host cell, a composition comprising a glucoamylase and methods of saccharifying the starch substrate using the glucoamylase. Moreover, the disclosure also relates to a process of producing fermentation products and a method for increasing starch digestibility in an animal as well as a method of producing a fermented beverage.

Described are a recombinant host cell, a composition comprising a glucoamylase and methods of saccharifying the starch substrate using the glucoamylase. Moreover, the disclosure also relates to a process of producing fermentation products and a method for increasing starch digestibility in an animal as well as a method of producing a fermented beverage.

A ruminant dietary supplement in the form of a pressed shelf-stable solid pill or bolus that effervesces when administered into the reticulum of a ruminant and releases calcium and dried active yeast.

A ruminant dietary supplement in the form of a pressed shelf-stable solid pill or bolus that effervesces when administered into the reticulum of a ruminant and releases calcium and dried active yeast.

Provided herein, inter alia, are methods and compositions for identifying major histocompatibility class II (MEW II) complex binding peptides.

Provided herein, inter alia, are methods and compositions for identifying major histocompatibility class II (MEW II) complex binding peptides.

A method for producing a shaped article comprising: a) providing a binder, which has been produced by a process of: (i) mixing (A) fungi or glucan and (B) starch with an alkaline agent to form an alkaline composition; and mixing the alkaline composition with an acidic agent to form the binder; or (ii) mixing (A) fungi or glucan and (B) starch with an acidic agent to form an acidic composition; and mixing the acidic composition with an alkaline agent to form the binder; (b) forming a binder composition by mixing the binder with filler material; (c) shaping the binder composition into a three-dimensional shape; and (d) curing the binder composition to form a shaped article having said three-dimensional shape, wherein steps c) and d) can be carried out simultaneously or separately, and wherein during one or both of steps c) and d) pressure is applied to the binder composition.

A method for producing a shaped article comprising: a) providing a binder, which has been produced by a process of: (i) mixing (A) fungi or glucan and (B) starch with an alkaline agent to form an alkaline composition; and mixing the alkaline composition with an acidic agent to form the binder; or (ii) mixing (A) fungi or glucan and (B) starch with an acidic agent to form an acidic composition; and mixing the acidic composition with an alkaline agent to form the binder; (b) forming a binder composition by mixing the binder with filler material; (c) shaping the binder composition into a three-dimensional shape; and (d) curing the binder composition to form a shaped article having said three-dimensional shape, wherein steps c) and d) can be carried out simultaneously or separately, and wherein during one or both of steps c) and d) pressure is applied to the binder composition.

A system for generating and using an assay key comprising growth conditions for a set of microorganisms for a set of diverse QAC-based culture media under a variety of incubation conditions known to modulate the effect of QAC on growth of some microorganisms in the set. Each culture medium is characterized by a pH and includes one or more QACs and one or more growth supplements. The set of culture media includes media comprising various combinations of pH, QAC type, QAC concentration, growth supplement type, and growth supplement concentration. The assay key can be used to identify a microorganism by inoculating a variety of growth media within the key and incubating the inoculated media under conditions within the key and comparing the resulting pattern of microorganism growth across the media and conditions with growth patterns for various known microorganisms across the media and conditions that are within the key.