A system for generating and using an assay key comprising growth conditions for a set of microorganisms for a set of diverse QAC-based culture media under a variety of incubation conditions known to modulate the effect of QAC on growth of some microorganisms in the set. Each culture medium is characterized by a pH and includes one or more QACs and one or more growth supplements. The set of culture media includes media comprising various combinations of pH, QAC type, QAC concentration, growth supplement type, and growth supplement concentration. The assay key can be used to identify a microorganism by inoculating a variety of growth media within the key and incubating the inoculated media under conditions within the key and comparing the resulting pattern of microorganism growth across the media and conditions with growth patterns for various known microorganisms across the media and conditions that are within the key.

Described herein is a method for producing a transgenic cell product wherein the gene of interest is operably linked to an inducible promoter other than AOX1. Production of the transgenic cell product is activated when the host cell is grown on a non-repressing carbon source for de-repressing the inducible promoter and an amount of an inducer compound selected from the group consisting of: formaldehyde; S-formylglutathione; S-hydroxymethyl glutathione; formic acid; an alkali metal salt of formic acid; and an alkaline earth metal salt of formic acid; sufficient to induce the inducible promoter is added to the host cell culture.

Described herein is a method for producing a transgenic cell product wherein the gene of interest is operably linked to an inducible promoter other than AOX1. Production of the transgenic cell product is activated when the host cell is grown on a non-repressing carbon source for de-repressing the inducible promoter and an amount of an inducer compound selected from the group consisting of: formaldehyde; S-formylglutathione; S-hydroxymethyl glutathione; formic acid; an alkali metal salt of formic acid; and an alkaline earth metal salt of formic acid; sufficient to induce the inducible promoter is added to the host cell culture.

The present invention relates to a recombinant microorganism, preferably a recombinant yeast, capable of producing caffeic acid comprising a heterologous gene coding for an enzyme of the hydrolase family capable of breaking, preferably of hydrolyzing, the caffeoyl-shikimate bond to produce caffeic acid from caffeoyl-shikimate. Said microorganism, preferably said recombinant yeast, may also be capable of producing ferulic acid from the caffeic acid obtained. The present invention also relates to a method for producing caffeic acid and a method for producing caffeic acid and/or ferulic acid, using microorganisms, preferably yeasts, according to the invention. Finally, the invention also relates to the use of microorganisms, preferably yeasts, according to the invention to produce caffeic acid and/or ferulic acid.

The present invention relates to a recombinant microorganism, preferably a recombinant yeast, capable of producing caffeic acid comprising a heterologous gene coding for an enzyme of the hydrolase family capable of breaking, preferably of hydrolyzing, the caffeoyl-shikimate bond to produce caffeic acid from caffeoyl-shikimate. Said microorganism, preferably said recombinant yeast, may also be capable of producing ferulic acid from the caffeic acid obtained. The present invention also relates to a method for producing caffeic acid and a method for producing caffeic acid and/or ferulic acid, using microorganisms, preferably yeasts, according to the invention. Finally, the invention also relates to the use of microorganisms, preferably yeasts, according to the invention to produce caffeic acid and/or ferulic acid.

This invention relates to the use of probiotic yeast cells that produce recombinant parathormone for therapeutic purposes, where probiotic cells that produce rhPTH developed for use in hypoparathyroidism treatment are first microencapsulated, placed into gelatin capsules and then orally administrated.

In an aspect, the present disclosure provides a novel microorganism having an ester degrading ability. In an aspect, the present disclosure provides a combination of novel microorganisms having an oil degrading ability. In an embodiment, the microorganism of the present invention includes a yeast belonging to the genus Yarrowia. In an embodiment, the microorganism of the present invention includes Yarrowia lipolytica. In an embodiment, the present disclosure provides a combination of a Burkholderia bacterium with a Yarrowia yeast. In an embodiment, provided is an oil degrading agent that comprises the microorganism of the present disclosure.

In an aspect, the present disclosure provides a novel microorganism having an ester degrading ability. In an aspect, the present disclosure provides a combination of novel microorganisms having an oil degrading ability. In an embodiment, the microorganism of the present invention includes a yeast belonging to the genus Yarrowia. In an embodiment, the microorganism of the present invention includes Yarrowia lipolytica. In an embodiment, the present disclosure provides a combination of a Burkholderia bacterium with a Yarrowia yeast. In an embodiment, provided is an oil degrading agent that comprises the microorganism of the present disclosure.

A long-chain composition has at least one long-chain alkane selected from the group consisting of C9-18 linear or branched alkanes and at least one long-chain carboxylic acid selected from the group consisting of C9-18 linear or branched, saturated or unsaturated aliphatic monocarboxylic acids. The mass ratio of the long-chain alkane to the long-chain carboxylic acid ranges from 1:1 to 40:1. The long-chain composition has a higher fermentation degree or higher substrate utilization rate and the like, when used as a starting material in the production of long-chain dibasic acids via fermentation.

A long-chain composition has at least one long-chain alkane selected from the group consisting of C9-18 linear or branched alkanes and at least one long-chain carboxylic acid selected from the group consisting of C9-18 linear or branched, saturated or unsaturated aliphatic monocarboxylic acids. The mass ratio of the long-chain alkane to the long-chain carboxylic acid ranges from 1:1 to 40:1. The long-chain composition has a higher fermentation degree or higher substrate utilization rate and the like, when used as a starting material in the production of long-chain dibasic acids via fermentation.