The present invention provides improved P450-BM3 variants with improved activity. In some embodiments, the P450-BM3 variants exhibit improved activity over a wide range of substrates.

The present invention provides improved P450-BM3 variants with improved activity. In some embodiments, the P450-BM3 variants exhibit improved activity over a wide range of substrates.

The subject invention provides materials and methods for producing and purifying sophorolipids (SLP). More specifically, the subject invention provides materials and methods for the purification of both hydrophobic and hydrophilic SLP molecules to a purity of, for example, at least 80% by weight, preferably at least 95% by weight, without using solvents or centrifugation. Advantageously, the subject invention is suitable for industrial scale production of purified SLP for use in, for example, cleaning products and detergents, and uses safe and environmentally-friendly materials and processes.

The subject invention provides materials and methods for producing and purifying sophorolipids (SLP). More specifically, the subject invention provides materials and methods for the purification of both hydrophobic and hydrophilic SLP molecules to a purity of, for example, at least 80% by weight, preferably at least 95% by weight, without using solvents or centrifugation. Advantageously, the subject invention is suitable for industrial scale production of purified SLP for use in, for example, cleaning products and detergents, and uses safe and environmentally-friendly materials and processes.

Unique carbon dioxide or lignocellulosic substrate is prepared and used to produce biosurfactants, based on different types of microorganism fermenting strains, using carbon dioxide or lignocellulose-based raw materials as the primary feedstock, subsequently utilizing a fermentation process to synthesize different structures of biosurfactants. This is a two-phase reaction where phase-one creates the feedstock for the phase-two reactions. The fermentation broth resulting from the phase-two reaction is the crude biosurfactant; it uses glycolipid or lipopeptide biosurfactant as the main component. The broth is then refined by filtration, then concentrated, and further purified to obtain the pure biosurfactant material. The biosurfactant of the present disclosure can be applied to industries such as petroleum, food or agriculture, daily chemicals, industrial chemicals, environmental protection, and medicine.

Method for the production of organic fertilizer comprising providing an organic source of ammonia gas, in particular air contaminated with ammonia gas, directing the air contaminated with ammonia gas through water and forming an ammonium hydroxide solution converting the ammonium into nitrate, dosing, under the control of a control system, the ammonium hydroxide solution to a recirculation stream from a second bio-reactor with bacteria suitable for converting the supplied ammonia into nitrite and nitrate. The amount of ammonium water fed by the control system to the acidic nitrate solution from the second bio-reactor depends on one or more measured pH values in the device.

Method for the production of organic fertilizer comprising providing an organic source of ammonia gas, in particular air contaminated with ammonia gas, directing the air contaminated with ammonia gas through water and forming an ammonium hydroxide solution converting the ammonium into nitrate, dosing, under the control of a control system, the ammonium hydroxide solution to a recirculation stream from a second bio-reactor with bacteria suitable for converting the supplied ammonia into nitrite and nitrate. The amount of ammonium water fed by the control system to the acidic nitrate solution from the second bio-reactor depends on one or more measured pH values in the device.

The present invention relates to a method including culturing a C.sub.1 compound-assimilating bacterium, which is a methylotroph, and/or a yeast by using a medium comprising, for example, a C.sub.1 compound and/or glycerol as a carbon source, to thereby produce EGT.

The present invention relates to a method including culturing a C.sub.1 compound-assimilating bacterium, which is a methylotroph, and/or a yeast by using a medium comprising, for example, a C.sub.1 compound and/or glycerol as a carbon source, to thereby produce EGT.

The present invention is related to a method for increasing the trans-specificity of a beta-carotene oxidase (BCO), particularly insect BCO, to be used in the production of vitamin A aldehyde (retinal) from conversion of beta-carotene, with at least about 75 to 100% of retinal in the trans-isoform.