The present disclosure pertains to compositions comprising aflibercept and methods for producing such compositions in chemically defined media and using chromatography to reduce amounts of certain aflibercept variants.

Provided herein is a serum-free media for culturing, such as cultivating, preparing and/or producing cells, such as immune cells, such as genetically engineered cells. Also provided is a liquid basal media and frozen supplements that can be used to produce serum-free media. The provided embodiments include methods for producing serum-free media and methods for culturing cells, such as activating, transducing, cultivating or expanded cells, in the presence of serum-free media.

The present disclosure pertains to compositions comprising anti-VEGF proteins and methods for producing such compositions.

The present disclosure pertains to compositions comprising anti-VEGF proteins and methods for producing such compositions.

Provided herein are improvements in cell culture methods and compositions related thereto. In partial particular, provided herein are compositions and methods, and kits increasing the cellular division times and viability. Also provided herein are compositions and method for performing electroporation where high levels of electroporation efficiency are achieved and where deleterious effect of electroporation on cells are decreased.

The present invention relates to a method or kit for producing cancer stem cell spheroids, and a method of screening of drugs for treating cancer cell resistance using the prepared cancer stem cell spheroid, and it can conveniently produce cancer stem cell spheroids, and the prepared cancer stem cell spheroid can be effectively utilized for screening drugs for treating cancer cell resistance.

A method of creating a protein rich conditioned medium. The method includes culturing mesenchymal stem cells in a container utilizing a first growth medium, allowing a time period for proliferation of the mesenchymal stem cells until a desired level of confluence is achieved in the container, discarding a supernatant from the container, adding a second medium to the container, incubating the mesenchymal stem cells, and collecting the conditioned medium. The method produces significantly higher quantities of byproducts secreted by the mesenchymal stem cells. Byproducts are usable for wound healing, disease treatment, cosmetic, or other beneficial effects when applied or otherwise delivered to a patient.

Primed cells, pluripotent stem cell aggregates and microtissues produced therefrom and uses of same for producing edible products.

A method for culturing bone marrow-derived mesenchymal stem cells which is capable of efficiently culturing the cells while minimizing the influence of animal-derived serum by use of a serum-free medium. The method includes inoculating cells extracted from a bone marrow fluid to a culture vessel, and performing medium replacement a plurality of times before initial passage, wherein in the inoculation, a medium supplemented with animal-derived serum is used, and in any of the plurality of times of medium replacement, the medium is exchanged with a serum-free medium without the use of the serum, followed by culture in the serum-free medium.

Disclosed herein are methods and compositions for producing a Wnt polypeptide under a serum-free condition. Also disclosed herein are methods of purifying the Wnt polypeptide from a serum-free condition.