The present invention relates to methods of modulating the mannose content of recombinant proteins.

The invention provides a method for culturing mammalian cells. The method provides greater control over cell growth to achieve high product titer cell cultures.

The present invention relates to methods of modulating the mannose content of recombinant proteins.

The invention provides a method for culturing mammalian cells. The method provides greater control over cell growth to achieve high product titer cell cultures by changing the temperature of the cell culture and/or by starving the cells in their asparagine supply.

Methods and composition for production of T cells are provided. Also provided are therapeutic methods using engineered T cells. For example, in certain aspects methods include preparing three dimensional cell culture compositions comprising stroma cells and hematopoietic stem or progenitor cells in a serum-free medium for producing T cells.

Cell culture media are provided herein as are methods of using the media for cell culture and protein production from cells.

Fetal bovine serum (FBS) is considered a major supplement in culturing cells. Due to ethical issues, high costs, and batch-to-batch variations, designing an alternative to the FBS in cell culturing is important. A biological supplement including of microbiota-derived postbiotics (MD-PBs) obtained from a fermentation medium of beneficial microorganisms isolated from natural microbiota sources was formulated, processed, combined, and used as an alternative to the FBS in stimulating cell proliferation and growth. According to results obtained from different cell lines and primary cells, the MD-PBs and exopolysaccharides (EPSs) alone and/or Bovine Serum Albumin (BSA) and Sericin stimulated cell growth better than the FBS and, thus the MD-PBs and the EPSs alone and/or the BSA and Sericin are used as a possible alternative to the FBS in cell culture experiments and cultured meat technology.

The present disclosure provides methods and compositions useful for the production of slaughter-free meat products, and the characterizations of the same. The slaughter-free meat products contain several points of distinction when compared to conventional meat procured by harvesting the tissue of a dead animal. Such points of distinction include, but are not limited to, significantly reduced or substantially no: steroid hormones, antibiotics, or microbial contamination; lower fat content; no vasculature; and extended shelf life both at room temperature and when refrigerated.

Provided herein is a method for assessing angiogenic effects of a test composition, the method including: providing human microvessel (MV) fragments selected to correspond to a desired patient profile; embedding the human MV fragments in a gel matrix of a three dimensional (3D) in vitro culture; providing serum free media to the 3D in vitro culture; contacting the 3D in vitro culture comprising embedded human MV fragments with a test composition; and assessing the angiogenic effects of the test composition by measuring at least one angiogenic growth parameter of the 3D in vitro culture comprising embedded human MV fragments. Also provided herein are 3D in vitro cultures useful in the disclosed methods.

The specification describes an improved serum-free animal cell culture medium, which can used for the production of a protein of interest. Ornithine, or a combination of ornithine and putrescine can be added to serum-free media or chemically defined media to improve viable cell density, to reduce cell doubling time, and to increase the production of a protein of interest.