A microfluidic device, method and kit for assaying and/or culturing cells are provided. The microfluidic device comprises a well block comprising a plurality of microwells; at least one cell culture layer selected from a first cell culture layer comprising a plurality of microchannels, each microchannel being aligned with one of the plurality of microwells and being in fluid communication with the aligned microwells; and a second cell culture layer comprising a plurality of cell culture chamber wells, each cell culture chamber well being aligned with one of the plurality of microwells and being in fluid communication with the aligned microwells, and a plurality of outlets, each of the plurality of outlets corresponding to one of the plurality of cell culture chamber wells; and a base block, wherein the at least one cell culture layer is sealably coupled between the well block and the base block, thereby allowing fluid communication between the plurality of microwells in the well block and the at least one cell culture layer.

Disclosed are a device for preparing stem cell spheroids, a method for preparing stem cell spheroids and a method for preserving stem cells which relate to the technical field of preservation and transportation of stem cells. The device for preparing stem cell spheroids disclosed herein includes a substrate. The substrate is provided thereon with culture chambers, each of which has side walls and a bottom wall made of a material incompatible with stem cells. By culturing stem cells with this device, the stem cells may be made into spheroids and thus form stem cell spheroids. The device may be used as a means of preserving or transporting stem cells which makes it possible for stem cells to still have a higher viability and pluripotency even after a long time of preservation, storage and transportation under ambient temperature condition in the form of spheroids.

A cell activation reactor and a cell activation method are provided. The cell activation reactor includes a body, a rotating part, an upper cover, a microporous film, and multiple baffles. The body has an accommodating space, which is suitable for accommodating multiple cells and multiple magnetic beads. The rotating part is disposed in the accommodating space and includes multiple impellers. The microporous film is disposed in the accommodating space and covers multiple holes of the accommodating space. The baffles are disposed in the body. When the rotating part is driven to rotate, the interaction between the baffles and the impellers separates the cells and the magnetic beads.

The invention relates to a cellular microcompartment comprising successively, organized around a lumen, at least one layer of pluripotent cells, an extracellular matrix layer and an outer hydrogel layer. The invention also relates to processes for preparing such cellular microcompartments.

Embodiments herein described provide antigen-presenting cell-mimetic scaffolds (APC-MS) and use of such scaffolds to manipulating T-cells. More specifically, the scaffolds are useful for promoting growth, division, differentiation, expansion, proliferation, activity, viability, exhaustion, anergy, quiescence, apoptosis, or death of T-cells in various settings, e.g., in vitro, ex vivo, or in vivo. Embodiments described herein further relate to pharmaceutical compositions, kits, and packages containing such scaffolds. Additional embodiments relate to methods for making the scaffolds, compositions, and kits/packages. Also described herein are methods for using the scaffolds, compositions, and/or kits in the diagnosis or therapy of diseases such as cancers, immunodeficiency disorders, and/or autoimmune disorders.

Provided herein are methods of culturing an adherent mammalian cell in a shake tube using a plurality of microcarriers and batch re-feed perfusion, and various methods that utilize these culturing methods.

To provide a cell culture container and a cell culture system that can perform cell sorting, culturing, cell processing, and the like in one space and a volume of the space can be varied to suit respective steps.

A cell culture container includes first molecules each bondable to target cells to be cultured, being immobilized to the container via a stimulus degradable linker, the container having a variable volume.

A cell culture system included a cell culture container, including first molecules each bondable to target cells to be cultured, being immobilized to the container via a stimulus degradable linker, the container having a variable volume, and a stimulus imparting device that imparts a stimulus to the stimulus degradable linker

This disclosure is directed to methods and systems for growing and/or harvesting cells. The method can include seeding cells on a plurality of carriers. The method can also include incubating the carriers under conditions suitable for cell growth.

A cell cultivation apparatus for cultivating microorganisms and growing cells at high density is provided. The apparatus includes a membrane comprising multiple surface features on a first side of the membrane for cell placement. The surface features comprising one or more compartments within which a cell can be located. The membrane includes a material that is at least partially permeable to gas. A second side of the membrane defines a gas region. The second side of the membrane is separated from the first side of the membrane by the membrane. The apparatus further includes a media region for receiving media. The compartments are configured to at least partially reduce media flow shear forces on one or more cells in the compartments. The surface features may be ridges, protrusions, fins, wells, and/or posts.

Apparatus and methods for culturing cells in a cell culture medium including magnetic beads in a fluid. A variable magnetic field can be applied to the magnetic beads to create shear forces on cells on the surface of the beads. In certain embodiments, a rotational force can also be applied to the magnetic beads and the magnetic force can counteract the rotational force.