The present disclosure relates to method for enhanced production of metabolite including but not limiting to isoprene and isoprenoid through chromosomal integration of genes belonging to MEP pathway. The disclosure further relates a host cell for the production of the said metabolite. The method of the present disclosure bypasses the cumbersome method of plasmid application for the production of metabolite. The disclosure also relates to a gene construct comprising MEP genes and auxotrophic markers and a vector comprising the said gene construct.

The present invention discloses a bacterium and an obtaining method and application thereof. The bacterium has a property of coproducing 1,3-propanediol and D-lactic acid. Further, the bacterium is Klebsiella oxytoca, including Klebsiella oxytoca PDL-5 CCTCC M 2016185. The obtaining method of the bacterium may be to obtain the bacterium by directly screening wild bacteria that satisfy conditions from the environment or performing gene engineering modification to wild bacteria. The present invention has the advantages that the bacteria can coproduce 1,3-propanediol and D-lactic acid through fermentation, the molar conversion rate and the concentration of the two products are very high, the types of byproducts are few, the concentration is low, the product extraction process is simplified, the high-efficiency biological production of 1,3-propanediol and D-lactic acid can be realized, and the industrial application prospect is very great.

The present disclosure relates to an acetohydroxy acid synthase variant in which the feedback inhibition to L-valine is released, a polynucleotide encoding the acetohydroxy acid synthase variant, an expression vector including the polynucleotide, a microorganism producing L-valine including the acetohydroxy acid synthase variant, and a method for producing L-valine using the microorganism.

The present application relates to a novel promoter and a method for producing target materials using the same. More specifically, the present application relates to a novel polynucleotide having promoter activity, a gene expression cassette, and a host cell comprising the same, and a method for producing target materials using the microorganism.

Among the various aspects of the present disclosure is the provision of methods, systems, and Rhodococcus sp. strains for the upcycling of polyethylene terephthalate) (PET). An aspect of the present disclosure provides for a system for waste PET valorization comprising: a microorganism capable of growing on PET hydrolysis products, such as PET hydrolysate. PET products from chemical hydrolysis, or alkaline hydrolysis products of PET as a carbon source.

A microorganism is transformed to be capable of producing guanidinoacetic acid (GAA). A method can be used for the fermentative production of GAA using such a microorganism. A corresponding method can be used for the fermentative production of creatine.

The present invention relates to a recombinant yeast having a reduced pyruvate decarboxylase activity, in the genome of which has been inserted: one or more nucleic acids encoding an acetolactate synthase or ALS, one or more nucleic acids en coding an acetolactate decarboxylase or ALD, andone or more copies of a nucleic acids encoding a NADH oxidase or NOXE.

Non-naturally occurring Zymomonas strains useful for the production of 2,3-butanediol are provided.

The present invention relates to an ALS inhibitor herbicide tolerant polyploid plants, such as B. napus or B. juncea plants, progeny and parts thereof comprising mutations in all acetolactase genes.

The invention relates to a method for enhancing the erythritol and/or erythrulose productivity and/or yield of an erythritol and/or erythrulose-producing yeast strain, such as Yarrowia lipolytica, comprising inhibiting in said yeast strain the expression or the activity of an endogenous L-erythrulose kinase and/or erythritol dehydrogenase. The invention also relates to a mutant yeast strain obtained by said method.