A bacterium of the species Corynebacterium glutamicum has the ability to excrete L-lysine, and contains in its chromosome a polynucleotide encoding a mutated polypeptide having the assumed function of an acyltransferase, hydrolase, alpha/beta hydrolase or of a pimeloyl-ACP methyl ester esterase. Also, a method is used for producing L-lysine using such bacterium.

Nucleic acid molecules encoding polypeptides having polyketide synthase activity have been identified and characterized. Expression or over-expression of the nucleic acids alters levels of cannabinoid compounds in organisms. The polypeptides may be used in vivo or in vitro to produce cannabinoid compounds.

A genetically engineered strain of Geobacter sulfurreducens exhibits improved function as a cathode biofilm compared to the wild type strain. The genetically engineered Geobacter sulfurreducens strain is capable of using carbon dioxide as a carbon source and electrical current as an energy source for producing a carbonaceous chemical using a reverse tricarboxylic acid pathway.

The invention relates to engineering of acetyl-CoA metabolism in yeast and in particular to production of acetyl-CoA in a non-ethanol producing yeast lacking endogenous gene(s) encoding pyruvate decarboxylase and comprising a heterologous pathway for synthesis of cytosolic acetyl-CoA.

The present disclosure provides methods of modulating the flux of carbon through the monoethylene glycol (MEG) biosynthesis pathway and one or more C3 compound biosynthesis pathways by expressing enzymes that are essential for improving C3 compounds and modulating other genetic aspects of MEG and C3 compound biosynthesis. The disclosure is further drawn to modified microbes comprising the disrupted sequences and overexpressed sequences, and compositions thereof.

The proposed solution refers to a host cell for the heterologous synthesis of at least one omphalotin compound comprising polynucleotides encoding for polypeptides involved in the omphalotin biosynthesis, an expression vector comprising said polynucleotides and a process for obtaining at least one omphalotin compound from said host cell.

Embodiments of the present invention relate to methods for the biosynthesis of di- or trifunctional C7 alkanes in the presence of isolated enzymes or in the presence of a recombinant host cell expressing those enzymes. The di- or trifunctional C7 alkanes are useful as intermediates in the production of nylon-7, nylon-7,x, nylon-x,7, and polyesters.

This disclosure describes biosynthesized compounds including anhydromevalonolactone and -methyl--valerolactone. This disclosure further describes biosynthetic methods for making these compounds. In some embodiments, the biosynthetic methods can include a combination of biosynthesis and chemical steps to produce -methyl--valerolactone. Finally, this disclosure described recombinant cells useful for the biosynthesis of these compounds.

This invention relates to metabolically engineered microorganisms, such as bacterial and or fungal strains, and bioprocesses utilizing such strains. These strains enable the dynamic control of metabolic pathways, which can be used to optimize production. Dynamic control over metabolism is accomplished via a combination of methodologies including but not limited to transcriptional silencing and controlled enzyme proteolysis. These microbial strains are utilized in a multi-stage bioprocess encompassing at least two stages, the first stage in which microorganisms are grown and metabolism can be optimized for microbial growth and at least one other stage in which growth can be slowed or stopped, and dynamic changes can be made to metabolism to improve the production of desired product, such as a chemical or fuel.

The present disclosure provides acyltransferases useful for synthesizing therapeutically important statin compound.