The present disclosure relates to a manufacturing process of sargramostim, which results in improved yield efficiency and output.

Beads containing a polymer containing uronic acid units having mercapto groups, in which the mercapto groups partly or entirely form disulfide bonds, are useful for encapsulating cells and microorganisms.

A method for removing iodine by using Deinococcus radiodurans having a gold nanoparticle synthesis ability is disclosed. More particularly, a method for removing radioactive iodine by adsorbing radioactive iodine onto gold nanoparticles synthesized in cells of Deinococcus radiodurans is disclosed. A recombinant microorganism having an enhanced radioactive iodine removal ability according to the present invention may selectively remove radioactive iodine present in various types of solutions at a high efficiency of 99% or higher, and thus may be very effective in removing radioactive iodine generated in large-scale hospitals, industries, nuclear facility accidents, and the like.

A method of preparing a stem-cell containing solution for dental implant treatment, comprising: storing a mixture of a blood sample and ethylenediaminetetraacetic acid (EDTA) at a temperature between 2 and 12 degrees Celsius for a time period of from 3 hours to 72 hours so as to have said mixture separates into an upper layer and a lower layer, wherein the upper layer comprises multiple somatic stem cells; collecting said first upper layer; after said collecting said upper layer, centrifuging said upper layer so as to form a first liquid and a pellet containing said somatic stem cells and said platelets; removing substantially all or a portion of the first liquid and leaving the pellet; re-suspending the pellet in a second liquid to form a stem-cell containing solution; and applying the stem-cell containing solution to a porous titanium-oxide layer on an outer thread of a dental implant.

The preset disclosure provides methods of culturing cells, e.g., pluripotent cells, multipotent cells, and/or immune cells (e.g., T cells and/or NK cells) in a medium comprising at least about 5 mM potassium ion, wherein the medium is not hypertonic. In some aspects, the medium is hypotonic. In some aspects, the methods disclosed herein increases the number of less-differentiated cells in the population of cells. In some aspects, the cultured cells are engineered, e.g., to comprise a chimeric antigen receptor or an engineered T cell receptor. In some aspects, the cells are administered to a subject in need thereof.

The ratio of zinc and cadmium is of significance for error-free proliferation and differentiation of cells. An understanding of the role of cadmium in the etiology of cancer offers possibilities to gain a better understanding of cancer as well as possibilities to prevent, treat and/or alleviate cancer. The concentration of Zn(II) in fetal serum, human serum from umbilical cord blood or healthy donors, and in avian serum including intact eggs and egg products has significance for the reliability and repeatability of cell and tissue culture method using said serum, or serum containing products, or eggs. Disclosed is a method in the manufacture of serum products, in particular serum products intended for use in in vitro culture of mammalian cells or tissues, wherein the concentration of Zn(II) is determined, and preferably adjusted to a desired interval. Different products consisting of or containing serum or serum fractions are also disclosed.

The present disclosure describes immunosuppressive mesenchymal stromal cells and exosomes secreted from immunosuppressive mesenchymal stromal cells, and methods for their preparation. The disclosure also describes methods for treating subjects or preventing subjects at risk for conditions by administering the immunosuppressive mesenchymal stromal cells or secreted exosomes. The present disclosure also describes kits for preparing immunosuppressive mesenchymal stromal cells and exosomes secreted from immunosuppressive mesenchymal stromal cells.

The present invention relates to a method of transporting a stem cell population, the method comprising transporting the stem cell population contacted with a liquid carrier. In addition, the present invention concerns a method of treating a subject having a disease, the method comprising topically administering a defined mesenchymal stem cell population to the subject, wherein the mesenchymal stem cell population is administered within about 96 hours from the time point the mesenchymal stem cell population has been harvested. Also concerned is a unit dosage comprising about 20 million cells, of about 15 million cells, of about 10 million cells, of about 5 million cells, of about 4 million cells, of about 3 million cells, of about 2 million cells, of about 1 million cells, of about 0.5 million cells, of about 0.25 million cells or of less than 0.25 million cells of a defined mesenchymal stem cell population.

A method for controlling the kinetics of a cultured cell includes: a culture step of culturing a cell adhered to the surface of a base material including titanium oxide having an anatase structure on the surface; an irradiation step of irradiating the surface with light in a wavelength range in which the titanium oxide exhibits photocatalytic activity during culture of the cell; and a control step of controlling the kinetics of the cell by controlling the irradiation amount of the light to the surface. The wavelength range may be 400 nm or less.

The present invention provides a mutated woodchuck post-transcriptional regulatory element (WPRE). In particular, the present invention relates to a mutated WPRE sequence that can efficiently express nucleotides of interest in a retroviral vector system. The present invention also relates to methods of delivering and expressing nucleotides of interest to a target cell.