The present invention discloses formulation of a serum-free medium used for human pluripotent stem cells, which comprises the following raw materials: inorganic salt components, organic components, amino acids and amino acid salts, energy substances and metabolic intermediates, vitamins and antioxidants, proteins and polypeptides, trace elements and chromogenic substances; while the culture process comprises the following steps: selecting a basic formulation, performing combination screening, identifying and evaluating results, and testing a new formulation of culture; and proportioning according to the following methods: adding aforesaid raw materials into 950 ml of water for injection, stirring gently until dissolved, and finally adding 2.438 g of sodium bicarbonate, and stirring gently until dissolved, and then adding 1 liter of water for injection, adjusting the pH to the desired value with 1 mol/L sodium hydroxide solution or 1 mol/L hydrochloric acid solution, finally filtering sterilized with 0.1 μm diameter filter under positive pressure, and storing the medium solution in dark place at 2° C.-8° C., the invention solves the problem of high cost of domestic import of serum-free formulation.

A method of efficiently recovering a large amount of exosomes from mesenchymal stem cells is provided. The method includes: a three dimensional culture step of three dimensionally culturing mesenchymal stem cells in a medium containing sugar by using a nonwoven fabric as a scaffold; a post-plateau culture step of further culturing for a certain period of time after the amount of the sugar consumed by the mesenchymal stem cells reaches a plateau; and an exosome recovery step of recovering exosomes from the mesenchymal stem cells. The mesenchymal stem cells are adipose-derived mesenchymal stem cells.

A cell proliferation inhibitor may include glutamic acid, and a method for regulating the proliferation of cells may use the same.

The disclosure features methods and compositions for differentiating stem cells into hematopoietic stem and progenitor cells (HSPC) and/or Natural Killer (NK) cells. The methods and compositions described herein are used to differentiate stem or progenitor cells having at least one gene-edit that is maintained in the differentiated cell. Also provided are differentiated cells produced using the methods and compositions described herein for therapeutic applications.

This application provides a method for culturing Lactobacillus plantarum, which comprises culturing Lactobacillus plantarum in a medium comprising molasses, a yeast extract and sucrose at a temperature from 32° C. to 37° C.

The invention provides a callus induction medium for blueberry tissue culture, it takes WPM medium as basic medium, comprises: 0.5-5.0 mg/L CPPU and 0.1-0.4 mg/L 2-ip. The present invention also provides a callus culture method of blueberry, the step thereof comprises: inoculating the blueberry explant into the above callus induction medium to conduct induction culture, to form blueberry callus. The present invention also discloses the medium combination and culture method to culture the above blueberry callus to blueberry tissue culture plant. For the above medium and culture method, the differentiation effect is good, efficiency is high, and can conduct continuous differentiation, and the effect to multiple varieties are all better.

A method for producing an epithelial cell sheet, comprising culturing cells derived from oral mucosal epithelial cells on a substrate in a serum-free medium, wherein the serum-free medium comprises (i) EGF protein or KGF protein, (ii) B-27 supplement, and (iii) a ROCK inhibitor.

The present invention relates to methods of upregulating the high mannose glycoform content of a recombinant protein during a mammalian cell culture by manipulating the mannose to total hexose ratio in the cell culture media formulation.

This invention relates, inter alia, to compositions of low serum or serum free media and methods for the expansion of T cell populations and methods for using such populations of cells. In some aspects, the invention relates to compositions and methods for the selective expansion of T cell subpopulations.

The present invention comprises a system and method for co-culturing glia cells and neurons in a combination of glia culture medium and neuron medium wherein the glia cells and neurons have cell morphology, cell reactions and/or cell interactions that exist in vivo after the co-culturing for up to about 21 days, up to about 30 days, up to about 40 days, up to about 45 days. Since the cell morphology, cell reaction and/or cell interaction of the co-culture are similar to those seen in vivo, the present system is capable of being configured as animal models for research, drug screening, testing and conducting clinical trials.