Disclosed are a construction method and application of a microorganism capable of realizing high production of lacto-N-neotetraose, belonging to the field of microbial genetic engineering. Coding genes of -1,3-acetyl glucosamine transferase, -1,4-galactosyl transferase and/or UDP-glucose 4 epimerase are over-expressed on the basis of a strain which is previously constructed by the team and is subjected to related-gene knockout, thus enabling the strain to have a synthesis capability of producing the lacto-N-neotetraose. The present disclosure accurately regulates the carbon flux of a metabolic pathway and relieves the metabolic stress by screening the high-efficiency -1,4-galactosyl transferase gene and regulating the expression of IgtA, Aa--1,4-GalT and galE in a lacto-N-neotetraose synthesis pathway in a combined manner. In a shake flask experiment, the lacto-N-neotetraose production capacity of Escherichia coli is 0.91 g/L. The lacto-N-neotetraose yield in a 3 L fermentation tank reaches 12.14 g/L. Therefore, the microorganism has an industrial application prospect.

The present disclosure relates generally to methods of producing multiple embryos from one or more donor embryos by serial multiplication, for example, by performing 3 or more rounds of multiplication, as well as the use of such methods in animal breeding. The present disclosure also relates to methods of producing multiple monozygotic embryos from a donor embryo which comprises embryonic cells which are developmentally equivalent to embryonic cells from a 16-cell embryo or a pre-compacted morula.

A fermentation method for mupirocin, the method comprising performing shake flask seed culture, seed tank culture, fermentation culture, feed culture. The fermentation method is not only suitable for industrial mass production, but also improves the fermentation potency of mupirocin, which can be stably maintained to be 8000 ug/ml or above.

Provided in the present invention is a cell freezing medium for clinical use. In particular, the cell freezing medium of the present invention comprises the following components: (1) human albumin; (2) cryoprotectant: the cryoprotectant comprises a combination of one or more of dimethyl sulfoxide, glycerol, and ethylene glycol; (3) a saline buffer; wherein the salt buffer is a solution containing Na.sup.+, K.sup.+, Mg.sup.+, Cl.sup., and CH.sub.3COO.sup. ions; (4) a vitamin; and (5) an amino acid, wherein the human albumin concentration is 1%-20% (w/v). The cell, after long-term cryopreservation with the freezing medium of the present invention, has a high viability, and the cellular efficiency maintains a high uniformity. The grade of purity of the freezing medium of the present invention is the pharmaceutical grade or USP grade; and the freezing medium is safe and reliable for clinical use, and can be used or conventional adherent and suspension cells.

A method of growing primary human epithelial cells, in particular human epithelial cells using a basal formula containing individual (a) amino acids, (b) vitamins, (c) trace elements, and (d) other organics such as linoleic acid. The basal medium may be a mixture of amino acids, vitamins, and salts that constitute the basic media that is used to culture epithelial cells over a number of population doublings, e.g., over at least one week, while maintaining a normal phenotype and exerting low stress on the cultured cells, and maintaining lineage heterogeneity.

The technology described herein is directed to engineered chemoautotrophic bacteria and methods of producing triacylglycerides. Also described herein are systems or bioreactors comprising said engineered bacteria.

To provide a microbial oil that contains eicosapentaenoic acid (EPA) at a high concentration and can effectively exert the function of EPA. A microbial oil containing EPA and docosahexaenoic acid (DHA), in which the content of EPA is 38% by area or greater relative to the total fatty acids and which has at least one fatty acid ratio selected from the group consisting of (a) to (c): (a) a ratio of the content of EPA to the content of DHA of 1.7 or more; (b) a ratio of the content of fatty acid(s) having 18 carbon atoms to the content of fatty acid(s) having 20 carbon atoms of 0.3 or less; and (c) a ratio of the content of fatty acid(s) having 18 carbon atoms to the content of fatty acid(s) having 22 carbon atoms of 1.5 or less.

A transfection reagent composition comprising: 30-60 MOL. % of a cationic lipid, or a pharmaceutical acceptable salt thereof; 10-60 MOL % of a structural lipid; 10-20 MOL % of a triglyceride; and 0.1 to about 10 MOL. % of a stabilizing agent, is provided. The transfection agent is effective in transfecting cells, particularly neurons, with siRNA, mRNA and plasmid nucleic acid, Sand maintaining viability of the cells as well as activity of the delivered nucleic acid.

The invention relates to a process for improving the solubility of dry cell culture media. Some dry powder cell culture media show poor dissolving properties and result in turbid solutions when they are dissolved in aqueous solutions. Using a stepwise procedure in which the amino acids present in the non-dissolving part are identified and added to a new batch in other particle sizes significantly reduces that problem.

The present disclosure relates to a recombinant E. coli strain that produces xylitol from xylose, a method for preparing the same, and a use thereof. Specifically, according to an embodiment of the present disclosure, there is provided a method for producing xylitol, wherein the method includes: culturing the recombinant E. coli strain transformed with an expression vector including a gene encoding a YahK enzyme and the recombinant E. coli strain on a substrate containing xylose (stage 1); and obtaining the xylitol from the culture cultured in stage 1 (stage 2).