A system for identifying Candida auris is disclosed. The system has two aspects. The first is a positive selection of C. auris based on C. auris's distinctive resistance to quaternary ammonium compounds (especially at elevated incubation temperatures). The second is a negative selection of C. auris based on C. auris's distinctive sensitive to tert-Butyl-hydroperoxide. C. auris can be identified in a sample through use of a positive-selection culture medium, which fosters C. auris colony growth while suppressing growth of other yeasts. The isolate can be confirmed as C. auris through use of a negative-selection culture medium, which suppresses C. auris colony growth while permitting growth of other yeasts.

A production method of low molecular weight ?-glucan includes: inoculating an activated Leuconostoc mesenteroides in a 5 L fermentor at a 10% inoculum. Fermentation broth is placed in the fermentor at an initial pH of 6.8-7.0, temperature of 25? C. to 28? C., stirring speed at 120 r/min, and fermented for 20-40 hours. Dextranase is added after 5-30 hours of fermentation at a dosage of 1/10,000 to 5/10,000 by volume. The molecular weight of ?-glucan is controlled within 10000D by the amount of enzyme added, and the total fermentation process is about 20-40 hours. After the reaction is terminated, the fermentation liquid is concentrated and dried to prepare dietary fiber products with a molecular weight of 500-5000D. The viscosity of the fermentation liquid and concentration of ?-glucan in the fermentation liquid may be reduced to promote the forward reaction, accelerate the sucrose conversion rate and increase the product yield.

The invention describes improved methods and compositions for producing a recombinant protein, e.g., an antibody, in mammalian cell culture. In addition, the invention provides improved cell culture media, including improved production media, feed solutions, and combination feeds, which may be used to improve protein productivity in mammalian cell culture.

Provided herein are methods, compositions, and systems for treating dental caries and a periodontal disease based on microbiome modulation through oral microbiome transplant (OMT). The original oral microbial composition was obtained from the dental plaque of healthy donors, and then modulated by growing a biofilm in vitro in a growth medium. The modulated oral microbial composition has a different microbial diversity than the original oral microbial composition. In the modulated oral microbial compositions, the abundance of some bacterial species changes, some new bacterial species are present, and some original bacterial species are no longer present. The modulated oral microbial composition is transplanted to the recipient's oral cavities through OMT to treat dental caries or a periodontal disease.

A mixed bacteria for promoting nodulation and nitrogen fixation of Robinia pseudoacacia and their application are provided. The mixed bacteria includes Kocuria sp. X-22, Microbacterium sp. X-26, and Bacillus sp. X-28, all of which have been preserved in China Center for Type Culture Collection, and the preservation numbers respectively are: CCTCC No: M 2019237; CCTCC No: M 2019238; CCTCC No: M 2019239. The mixed bacteria are watered directly around the seedlings of Robinia pseudoacacia. Compared with the single bacteria control group and the sterile control group, the disclosure can produce synergistic superimposing effects, significantly improve the nodulation rate and symbiotic nitrogen fixation of the Robinia pseudoacacia, and promote the photosynthesis of the Robinia pseudoacacia.

The present invention relates to the technical field of microbial fermentation engineering, and specifically to a method for producing psicose 3-epimerase by high-density fermentation. In view of the low expression level of psicose 3-epimerase and other problems existing in the current fermentation, by controlling the feeding rate in the fermentation process, improving the culture temperature in the middle and late stages of fermentation and other measures in the present invention, the OD value during the fermentation with recombinant Bacillus subtilis and the total enzyme activity of psicose 3-epimerase in the fermentation broth are significantly increased, the expression of psicose 3-epimerase is markedly improved, and the production cost of allulose is reduced. Therefore, the present invention has a very broad prospect of application in industry.

The present invention relates to animal protein-free cell culture media comprising polyamines and a plant- and/or yeast-derived hydrolysate. The invention also relates to animal protein-free culturing processes, wherein cells can be cultivated, propagated and passaged without adding supplementary animal proteins in the culture medium. These processes are useful in cultivating cells, such as recombinant cells or cells infected with a virus, and for producing biological products by cell culture processes.

The present disclosure relates to the technical field of microorganisms, in particular to Lactobacillus gasseri HMV18 and a secreted protein and an application thereof. The Lactobacillus gasseri HMV18 is preserved in the China Center for Type Culture Collection on Jul. 11, 2019 with a preservation number of CCTCC NO: M 2019538, and a preservation address of Wuhan University, Wuhan, China. The protein extracted from the Lactobacillus gasseri HMV18 has antibacterial and anti-tumor effects, but basically has no effect on normal myocardial cells, so the Lactobacillus gasseri HMV18 can be applied to preparation of antibacterial and anti-tumor products.

The present invention relates to animal protein-free cell culture media comprising polyamines and a plant- and/or yeast-derived hydrolysate. The invention also relates to animal protein-free culturing processes, wherein cells can be cultivated, propagated and passaged without adding supplementary animal proteins in the culture medium. These processes are useful in cultivating cells, such as recombinant cells or cells infected with a virus, and for producing biological products by cell culture processes.

The invention describes improved methods and compositions for producing a recombinant protein, e.g., an antibody, in mammalian cell culture. In addition, the invention provides improved cell culture media, including improved production media, feed solutions, and combination feeds, which may be used to improve protein productivity in mammalian cell culture.