The present invention discloses a genetically engineered bacteria, which is E. coli integrated with lysogenic ?DE3, and lacZ gene is completely inactivated, but does not affect exogenous protein expression of the genetically engineered bacteria. The present invention also discloses a method for culturing the genetically engineered bacteria, and a method for preparing human milk oligosaccharides using the same, and use of the genetically engineered bacteria. The genetically engineered bacteria of the present invention can efficiently produce human milk oligosaccharides, such as 2-fucosyllactose, and have wide industrial application prospects.

The specification describes a composition comprising an improved eukaryotic cell culture medium, which can be used for the production of a protein of interest. TaXULne can be added to the serum-free media or chemically-defined media to increase the production of a protein of interest. Methods for recombinantly expressing high levels of protein using the media compositions are included.

The specification describes a composition comprising an improved eukaryotic cell culture medium, which can be used for the production of a protein of interest. TaXULne can be added to the serum-free media or chemically-defined media to increase the production of a protein of interest. Methods for recombinantly expressing high levels of protein using the media compositions are included.

Provided is a method of producing a freeze-dried probiotic composition as well as a method of producing a probiotic raw material which involves culturing of a Streptococcus salivarius strain. Furthermore, a corresponding probiotic composition and a corresponding probiotic raw material is provided.

Provided is a method for the production of single cell protein (SCP) comprising anaerobic fermentation of Clostridia bacterium on a fermentation medium comprising a gas, wherein said gas provides a source of energy and carbon for said bacterium. Further provided is a single cell protein produced by the method as disclosed herein.

The specification describes a composition comprising an improved eukaryotic cell culture medium, which can be used for the production of a protein of interest. Taurine can be added to serum-free media or chemically-defined media to increase the production of a protein of interest. Methods for recombinantly expressing high levels of protein using the media compositions are included.

Disclosed are a strain for producing nattokinase and a production method therefor. In particular, the present invention involves a novel strain capable of producing nattokinase, i.e. Bacillus subtilis natto ST-1086, deposited at the China General Microbiological Culture Collection Center under CGMCC No. 17895. The present invention further involves a method for producing a nattokinase product by means of using the novel strain CGMCC No. 17895 of the present application, wherein the resulting nattokinase product can be used as a drug for dissolving thrombi. The present invention further involves the use of the nattokinase product of the present application for preparing a composition for dissolving thrombi and in a method for treating thrombi.

The present invention relates to animal protein-free cell culture media comprising polyamines and a plant- and/or yeast-derived hydrolysate. The invention also relates to animal protein-free culturing processes, wherein cells can be cultivated, propagated and passaged without adding supplementary animal proteins in the culture medium. These processes are useful in cultivating cells, such as recombinant cells or cells infected with a virus, and for producing biological products by cell culture processes.

The disclosure relates to canola meal extracts (CMEs), and methods of making said CMEs. Also provided herein are compositions and culture media comprising said CMEs and use of said CMEs, compositions, and culture media for microbial fermentation across a broad class of microbes. The disclosure further relates to use of said CMEs and compositions as a partial or complete replacement for other organic extracts such as yeast extract.

A Lactobacillus plantarum 121-5 is disclosed. The preservation number is CGMCC No. 27418, and the Lactobacillus plantarum is preserved in the China general microbiological culture collection center on May 24, 2023. The Lactobacillus plantarum 121-5 has high beta-glucosidase activity, can efficiently convert polydatin to prepare resveratrol, and has short conversion time and high conversion rate. Meanwhile, the conversion condition is mild, safe and reliable, and the large-scale preparation is easy. The strain is directly applied to fermentation of foods and medicines (edible and medicinal plants or fruits and vegetables) containing polydatin, so that the resveratrol content in a fermentation product is improved, and the biological activity of the product is improved.