A method for preparing a biological age reversing preparation, comprising the steps of: placing cytotoxic T lymphocytes, a cell mitogen, a cytokine, and an immunologic adjuvant in a liquid cell culture medium in a culture container for co-culture to obtain an immune cell culture, and isolating a population of cells with immune activity from the immune cell culture to obtain the biological age reversing preparation. By using the biological age reversing preparation, the biological age can be reversed significantly. The biological age reversing preparation comprising 10.sup.10 cytotoxic T lymphocytes is used three times within a week, resulting 1.3 years of biological age reversal after the preparation is continuously used for 3 months, and 4.5 years of biological age reversal after the preparation is continuously used for 9 months.

The present invention relates to a cell culture scaffold, and provides a cell culture scaffold which has a hydrogel structure comprising alginate and cellulose extracted by means of algae decellularization and which enable the stable growth of cells even at low cost while having a simple preparation.

A method for promoting exosome secretion from animal cells is provided. The method includes treating the animal cells with rose-derived exosomes. The method can promote the secretion of exosomes from animal cells by treating the animal cells with rose-derived exosomes.

The present disclosure relates to a cell culture composition containing an extract from microalgae of the Thraustochytrid family, and methods of preparation and use thereof.

A technology regarding the production, formulation and use of conditioned media and the factors included therein is disclosed. The conditioned media may be inoculated with animal cells, plant cells and any combination thereof. The inoculations may occur simultaneous or at different times. Cells retrieved from different areas of the animal and/or the plant may also be cultured together to form conditioned media and associated growth factors.

A technology regarding the production, formulation and use of conditioned media and the factors included therein is disclosed. The conditioned media may be inoculated with animal cells, plant cells and any combination thereof. The inoculations may occur simultaneous or at different times. Cells retrieved from different areas of the animal and/or the plant may also be cultured together to form conditioned media and associated growth factors.

Provided is an additive composition for culture media, comprising a first component comprising at least one sample extract prepared from at least one natural substance, wherein the at least one natural substance is selected from a group consisting of bird's nest (BN), ginseng, Ling Zhi, versicolor mushroom, Cordyceps sinensis (CS), Ashwagandha (ASH), and combinations thereof. The additive composition can be acted as an alternative source of growth factors and hormones for cell culture media to improve consumer acceptance and/or to minimize the production costs.

Methods of farming, domesticating, or cultivating truffle fungi, including isolation and cultivation methods for producing pure truffle fungi, or mini-truffles, typically a Tuber spp. or an Iamai spp. Examples of truffle include Tuber melanosporum, Tuber magnatum, Tuber aestivum, Tuber uncinatum, Tuber borchii, Imaia spp., Tuber macrosporum, Tuber gibbosum, Tuber oregonense, and Tuber lyonii. The isolated pure truffle may then be cultivated on a nutrient substrate, which may be, for example, a fruit, nut, grain, or a portion thereof, resulting in a truffle-flavored food product. Non-limiting examples of such substrates include rye, barley, lentil, wheat, rice, soybeans, pecan, hazelnut, pine nut, English walnut, coffee beans, mustard, cacao, sesame, sunflower, grapes, blackberries, blueberries, cherries, kiwi, mango, raspberries, and huckleberries.

Methods of farming, domesticating, or cultivating truffle fungi, including isolation and cultivation methods for producing pure truffle fungi, or mini-truffles, typically a Tuber spp. or an Iamai spp. Examples of truffle include Tuber melanosporum, Tuber magnatum, Tuber aestivum, Tuber uncinatum, Tuber borchii, Imaia spp., Tuber macrosporum, Tuber gibbosum, Tuber oregonense, and Tuber lyonii. The isolated pure truffle may then be cultivated on a nutrient substrate, which may be, for example, a fruit, nut, grain, or a portion thereof, resulting in a truffle-flavored food product. Non-limiting examples of such substrates include rye, barley, lentil, wheat, rice, soybeans, pecan, hazelnut, pine nut, English walnut, coffee beans, mustard, cacao, sesame, sunflower, grapes, blackberries, blueberries, cherries, kiwi, mango, raspberries, and huckleberries.

The invention discloses a method for preparing the ginsenoside preparation by biological engineering technology, wherein the ginsenoside preparation comprises at least one of the saponin-enriched preparation and the saponin-enriched syrup; the compound bio-enzyme preparation prepared by the combined bacteria preparation of yeast and lactic acid bacteria provided by the invention is rich in amino acid residues, amides, coenzymes and active groups such as lactic acid and linoleic acid; and the ginsenoside preparations containing ginsenosides can be prepared by adding monosaccharides or polysaccharides to the compound bio-enzyme preparations, providing new directions and ideas for obtaining ginsenosides and their products.