The present disclosure provides, in part, a cell culture medium supplement comprising at least one plant protein homologue of a serum protein, a cell culture medium comprising a serum-free base medium and one or more plant based proteins, and methods of growing cells in vitro and of producing cultured meat using the cell culture medium.

The present invention refers to a coating composition comprising a suspension of a microorganism, wherein the suspension comprises a pigmented vegetative cell, an unpigmented vegetative cell and a pigmented chlamydospore of the microorganism, and a surfactant ranging from 130 g/mol and 1500 g/mol. The invention further relates to a method for the preparation of the coating composition, a method for application coating one or more coating pre-layers and curing a material, a coated material obtainable by this method and a method for refreshing the coating of the coated material.

A technology regarding the production, formulation and use of conditioned media and the factors included therein is disclosed. The conditioned media may be inoculated with animal cells, plant cells and any combination thereof. The inoculations may occur simultaneous or at different times. Cells retrieved from different areas of the animal and/or the plant may also be cultured together to form conditioned media and associated growth factors.

Provided are a scaffold including a plant protein and in-vitro meat produced by using the scaffold. Considering that the scaffold consists of a plant protein, cultured muscles or adipose tissues may be ingested together with the scaffold without being separated therefrom. By adjusting a ratio of a muscle cell and an adipocyte, in-vitro meat having desirable texture may be produced, and since various types of cells may adhere, proliferate, and differentiate on the scaffold, such a scaffold may be effectively utilized for mass production of in-vitro meat.

The present invention relates to a method for preparing an induced pluripotent stem cell line from mesenchymal stem cells; and an induced pluripotent stem cell line (deposit number: KCLRF-BP-00318) obtained thereby. Specifically, the method for preparing an induced pluripotent stem cell line, of the present invention, comprises the steps of: (a) obtaining mesenchymal stem cells from a human umbilical cord; (b) forming, from the mesenchymal stem cells, a colony with a medium for dedifferentiation containing an Ecklonia cava extract; and (c) obtaining an induced pluripotent stem cell line by sub-culturing the colony. The induced pluripotent stem cell line according to the present invention was first established by the present inventors, and the pluripotent stem cell line of the present invention can be differentiated into various cells and can treat various diseases or disorders through cell transplant therapy.

The present invention discloses a serum-free medium for full suspension culture of MDCK cells and a preparation method of the serum-free medium. The serum-free medium for full suspension culture of the MDCK cells comprises basic metabolic nutrients, nucleotide, vitamins, inorganic salts, a shear force protective agent, a cell clustering resisting agent, a pH buffer agent, a pH indicator, an influenza virus proliferation accelerant and other additives. The preparation method of the serum-free medium for the full suspension culture of the MDCK cells comprises the following steps: 1) preparing a mixed solution: dissolving and mixing raw materials; and 2) regulating pH: regulating the pH of the mixed solution to 6.3 to 6.7, and setting a constant volume to obtain the serum-free medium for the full suspension culture of the MDCK cells. The medium supports the high-density full-suspension culture of the MDCK single cells, greatly shortens a time for educating the MDCK cells from adherent cells into the serum-free full suspension cells, and is applicable to the mass production of biological products, and particularly veterinary biological products.

The present invention relates to: a cosmetic composition for improving the condition of the skin, containing, as active ingredients, a cell culture medium, an epidermal growth factor and a bovine serum albumin; a method for improving the condition of the skin by using the same; and a method for preventing or treating skin diseases. According to the present invention, the cosmetic composition can exhibit excellent wrinkle-reducing and wound-healing effects even when only a small amount of EGF is used, and thus can be useful in improving the condition of the skin or in preventing or treating skin diseases.

The present invention is related to a therapeutic compound and its application in repairing diabetes-related cardiac injuries, particularly with providing a therapeutic composition containing the epigallocatechin gallate (EGCG) of green tea and the adipose-derived stem cells. The epigallocatechin gallate (EGCG) of green tea can enhance the ability of the adipose-derived stem cells to repaired damaged tissue. And the application is used for repairing the diabetes-related cardiac injuries.

A method of culturing a cell population containing cartilage-derived cells positive for expression of Tie2 (cartilage-derived Tie2-positive cells), the method including culturing a cell population containing cartilage-derived Tie2-positive cells in a culture medium containing at least one kind of Tie2 expression enhancer other than growth factors (e.g., an extract derived from a plant of the genus Cinnamomum). This culturing method is preferably performed in cultureware having a culture surface coated with a coating agent (e.g., a polylysine-containing agent).

The method of making a three-dimensional, leaf-based scaffold for three-dimensional cell cultures includes washing a quantity of Ficus religiosa leaves, then treating the washed Ficus religiosa leaves in a sodium hydroxide solution to obtain alkali-treated Ficus religiosa leaves. The alkali-treated Ficus religiosa leaves are washed, and then superficial tissue is removed from the alkali-treated Ficus religiosa leaves to obtain Ficus religiosa leaf skeletons. The Ficus religiosa leaf skeletons are dried and then consecutively immersed in distilled water, a phosphate buffer saline solution, and plain Dulbecco's modified Eagle's medium (DMEM) to form the three-dimensional scaffolds for three-dimensional cell cultures. Each three-dimensional scaffold can be used for growing three-dimensional cell cultures, such as human mesenchymal stem cell cultures.