The present invention relates to a medium composition for production of botulinum toxin and, more particularly, to a medium composition for culture of Clostridium sp. capable of producing botulinum toxin. The medium composition of the present invention comprises at least one plant-derived peptone selected from the group consisting of a garden pea hydrolysate, a cotton seed hydrolysate and a wheat gluten hydrolysate. When the medium according to the present invention, which contains plant-derived peptones and minerals, is used for culture of Clostridium botulinum, the growth rate of the bacterium in the medium is about 1.5-2 times higher than that in the medium that is in current use. In addition, when botulinum toxin is produced by culturing the bacterium in the medium, infection with transmissible spongiform encephalopathy (TSE) or the like can be prevented by blocking introduction of animal-derived components.

The present invention discloses the potential of Calebin A in attenuating high fat diet (HFD) induced hepatic steatosis in mammals.

The present invention relates to: a method for preparing an induced pluripotent stem cell line from mesenchymal stem cells; and an induced pluripotent stem cell line (deposit number: KCLRF-BP-00318) obtained thereby. Specifically, the method for preparing an induced pluripotent stem cell line, of the present invention, comprises the steps of: (a) obtaining mesenchymal stem cells from a human umbilical cord; (b) forming, from the mesenchymal stem cells, a colony with a medium for dedifferentiation containing an Ecklonia cava extract; and (c) obtaining an induced pluripotent stem cell line by sub-culturing the colony. The induced pluripotent stem cell line according to the present invention was first established by the present inventors, and the pluripotent stem cell line of the present invention can be differentiated into various cells and can treat various diseases or disorders through cell transplant therapy.

The present application provides: a suppressor of pluripotent stem cell aggregation for use in suspension culture of pluripotent stem cells, comprising a cell cycle arresting agent; a method for producing a pluripotent stem cell aggregate(s), comprising a step of subjecting pluripotent stem cells to suspension culture in a culture medium comprising the suppressor; a pluripotent stem cell aggregate obtained by the method; and a composition for culture of pluripotent stem cells comprising a cell aggregate(s) of pluripotent stem cells, a culture medium, and a cell cycle arresting agent. According to the method, it is possible, in suspension culture of pluripotent stem cells, to produce a cell aggregate(s) with high cell viability and appropriate size in a high yield while maintaining the undifferentiated state of the cells and suppressing the aggregation.

Production of etanercept using perfusion methods achieves attractive yields of properly folded protein. Desired temperature, feed media, titers and percent correctly folded protein are disclosed.

The present invention relates to an organoid and, more specifically, to an organoid and a use thereof, the organoid being produced using a carrier for cell culture which comprises microcapsules containing gelatin, a natural polymer, an oil, and an oil thickener. When used as a carrier for cell culture in culturing cells, the microcapsules containing a natural oil, according to the present invention, have the effects of improving adhesion and survival of the cells and inducing maturation of the cultured cells. The organoid produced by culturing cells using the carrier for cell culture has been confirmed to have the function of the organ concerned and, when treated with a drug, react to the toxicity of the drug and thus may be variously employed in the development of new drugs, disease research, and the field of artificial organ development.

A liquid biofertiliser which comprises Azospirillum brasilense CECT 5802 and Pantoea dispersa CECT 5801 strains. A method for stimulating plant growth, which comprises applying said liquid biofertiliser to said plant. A method for obtaining a liquid biofertiliser, which comprises culturing said strains in first liquid culture mediums and adding the culture broths obtained to a second liquid culture medium.

The present disclosure relates to a method for the cultivation of primary cells. The primary cells are cultivated in a serum free medium supplemented with peptides and peptones derived from plant or vegetable sources. The method for the cultivation of primary cells may be one step in a method for the amplification of viruses, such as poxviruses.

Disclosed herein is a method for producing exosomes, which includes cultivating avian embryo-derived mesenchymal stem cells in a culture medium containing 2,3,4′,5-tetrahydroxystilbene-2-O-β-D-glucoside, so as to obtain a cell culture of AMSCs, and subjecting the cell culture of AMSCs to a separation treatment, so as to obtain the exosomes. An exosome produced by the method, and a method for improving a skin condition using the exosome are also disclosed.

Disclosed is a L-isoleucine-producing Corynebacterium glutamicum fermentation medium, comprising a basal medium and a growth factor, wherein the growth factor consists of choline, betaine and vitamin B6, and the contents of each ingredient in the fermentation medium are: 0.2-1 g/L choline, 0.25-0.5 mg/L betaine, and 0.05-0.3 mg/L vitamin B6. Also disclosed is a method for cultivating the L-isoleucine-producing Corynebacterium glutamicum, comprising: inoculating the L-isoleucine-producing Corynebacterium glutamicum onto the fermentation medium, wherein the volume of the bacteria liquid accounts for 5-20% of the volume of the fermentation medium, adjusting the pH to 6.5-7 with aqueous ammonia, controlling the dissolved oxygen to 30-50%, and fermenting for 25-30 h; then decreasing the dissolved oxygen to 15-25%, and feeding a 50-80% glucose solution into the fermentation broth to control the residual sugar at 3-4%, continuing the fermentation until 60-70 hours, then terminating the fermentation, and controlling the temperature of the overall fermentation process at 29-33° C.