The present invention relates to methods of modulating (e.g., reducing) the mannose content, particularly high-mannose content of recombinant glycoproteins.

A microbial-based composition provides a co-cultured microorganism consortium in culture medium that includes one or more Acetobacter sp., Bacillus sp., Bifidobacterium sp., Enter ococus sp., Gluconacetobacter sp., Lactobacillus sp., Rhodopseudomonas sp., Saccharomyces sp., Pichia sp., and Trichoderma sp., as well as a carbon source and chlorine-free water. In some embodiments, the microbial-based composition is useful in the agricultural industry as a plant growth promoting and silage-enhancing agent. For plant growth promotion applications, the microbial-based composition may be applied to the foliar surface of a plant or to the plant growth medium, such as soil or hydroponic solution, surrounding the plant. In silage operations, the microbial-based composition may be applied to a cut plant product during one or more stages in the silage process.

The present invention relates to: a cosmetic composition for improving the condition of the skin, containing, as active ingredients, a cell culture medium, an epidermal growth factor and a bovine serum albumin; a method for improving the condition of the skin by using the same; and a method for preventing or treating skin diseases. According to the present invention, the cosmetic composition can exhibit excellent wrinkle-reducing and wound-healing effects even when only a small amount of EGF is used, and thus can be useful in improving the condition of the skin or in preventing or treating skin diseases.

A technology regarding the production, formulation and use of conditioned media and the factors included therein is disclosed. The conditioned media may be inoculated with animal cells, plant cells and any combination thereof. The inoculations may occur simultaneous or at different times. Cells retrieved from different areas of the animal and/or the plant may also be cultured together to form conditioned media and associated growth factors.

The present invention relates to a method of preparing xylitol by using a xylose secondary mother liquor through fermentation. In the method, fermentation treatment is performed on a mixed liquid of the xylose secondary mother liquor and a xylose concentrate, and the xylose secondary mother liquor is subsequently batch-fed instead of glucose in the fermentation process. Thus, a glucose content in the fermentation system is stabilized, and some xylose is provided at the same time. In this way, the use amount of glucose and costs are reduced, and the system xylose can be relatively stabilized, thus improving the entire conversion rate. In the present invention, adding the xylose secondary mother liquor by mixed fermentation and batch feeding and the like enables the contents of heterosaccharides such as arabinose and mannose in the fermentation system to be always within a range of metabolization by fermenting strains, facilitating subsequent purification and improving the xylitol purity.

The invention is directed to tools, compositions, and methods for the cultivation of microorganisms in culture media that is devoid of animal-derived materials such as blood, and, in particular, to compositions of meat-free media.

The present invention relates to animal protein-free cell culture media comprising polyamines and a plant- and/or yeast-derived hydrolysate. The invention also relates to animal protein-free culturing processes, wherein cells can be cultivated, propagated and passaged without adding supplementary animal proteins in the culture medium. These processes are useful in cultivating cells, such as recombinant cells or cells infected with a virus, and for producing biological products by cell culture processes.

Systems and methods for producing cell cultured food products. The cultured food products include sushi-grade fish meat, fish surimi, foie gras, and other food types. Various cell types are utilized to produce the food products and can include muscle, fat, and/or liver cells. The cultured food products are grown in pathogen-free culture conditions without exposure to toxins and other undesirable chemicals.

A technology regarding the production, formulation and use of conditioned media and the factors included therein is disclosed. The conditioned media may be inoculated with animal cells, plant cells and any combination thereof. The inoculations may occur simultaneous or at different times. Cells retrieved from different areas of the animal and/or the plant may also be cultured together to form conditioned media and associated growth factors.

The invention relates to methods for inducing sporulation in Bacillus coagulans strains wherein excessive sporulation is induced by presence of certain nutrients and minerals up to a level of 109 spores/ml. The spores are tadpole-like endospores and the method discloses specific parameters of growth medium and incubation conditions to be used to induce sporulation in Bacillus coagulans strains.